Stoke Therapeutics is a biotechnology company that focuses on developing RNA-targeted medicines to treat severe genetic diseases. Founded in 2014, Stoke Therapeutics utilizes its proprietary technology platform to address the underlying causes of a range of genetic disorders. The company's innovative approach has the potential to transform the treatment landscape for these conditions, offering new hope to patients and their families.

Research and Development Specialist

Sr. Scientist, Formulation and GMP Manufacturing

Research Scientist in Neurology

Pharmacology Expert

In Vivo Research Specialist

Senior Scientist, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly skilled and motivated Senior Analytical Scientist to join our Analytical and Formulation Sciences team. The ideal candidate will have strong hands-on experience in UPLC method development, and mass spectrometry-based characterization of oligonucleotide and conjugates. This role is critical to supporting the development of innovative oligonucleotide-based therapeutics. This position will report into the Director, CMC. Key Responsibilities: Develop, optimize, and validate UPLC methods for the analysis of oligonucleotides and their conjugates. Conduct mass spectrometry (LC-MS, HRMS, MS/MS) analyses to characterize oligonucleotide and its conjugates. Interpret analytical data to support product development, stability studies, and release testing. Collaborate with cross-functional teams including Process Development, Quality, and Regulatory Affairs. Author technical reports, method development summaries, and regulatory submissions (e.g., IND, BLA). Perform routine formulation preparation and characterization of oligonucleotide drug products for preclinical studies. Required Skills &amp; Experience: Ph.D. in Analytical Chemistry, Biochemistry, Pharmaceutical Sciences, or a related field with 6+ years of relevant industry experience; or M.S. with 10+ years of experience; or Bachelor’s with 16+ years of experience. Proven experience in UPLC method development for oligonucleotides or nucleic acid-based therapeutics. Strong expertise with mass spectrometry for structural and quantitative analysis of oligonucleotide and conjugates is desired. Strong data analysis skills and proficiency with analytical software (e.g., Empower, MassLynx, Xcalibur). Excellent communication, documentation, and organizational skills. Ability to work independently and collaboratively in a fast-paced environment. Knowledge of ICH guidelines and regulatory expectations for analytical method validation. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is based in Bedford, MA. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Analytical Scientist

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a Director, Engineering &amp; Facilities to manage the ongoing operations and labs within our current headquarters in Bedford. Importantly, this person will also oversee and manage alongside the Chief Financial Officer the ongoing search for a potential new headquarters for Stoke. Therefore, this role is critical not only to develop an efficient, safe, and scalable environment across both office and lab settings, but also an opportunity to play an important role in Stoke’s potential move into our new home. It's a hands-on, strategic opportunity for someone who thrives in a fast-paced environment. This position will report into the Chief Financial Officer. Key Responsibilities: Help manage the process for Stoke’s potential new headquarters search. Project manage construction of new offices, if applicable. Oversee technical aspects of new lab construction, including ensuring sufficient lab space and understanding building requirements. Oversee day-to-day operations of the facilities and the functioning of all building systems. Support the design, construction, startup and operation of all locations. Supervise external real estate, architectural, engineering and contractor relationships. Proactively engage with internal cross-functional teams to achieve site goals and ensure the facility is operating smoothly and in compliance with all health and safety standards. Develop and formalize approval of documents related to site operations including SOPs, safety training, policies and procedures. Ensure building operations comply with all local building codes, zoning laws and regulations. Establish and maintain relationships and contracts with external vendors for services such as ULS inventory management, Airgas, FedEx, and hazardous waste disposal. Review fixed asset inventory and update as required. Maintain and improve site compliance related to environment, health, and safety. Develop and implement processes necessary to operate the facility, including non-GMP cleaning/housekeeping, badging/security, and pest control. Make recommendations to promote efficiencies and apply a proactive approach to potential operational/environmental hazards/risks. Manage projects in support of space planning and expansion across all locations to meet hiring requirements. Develop and maintain budgets and long-range facilities plans based on company growth and future needs. Additional responsibilities as assigned. Required Skills &amp; Experience: Bachelor's degree in engineering or related discipline preferred or equivalent combination of education and work-related experience. Minimum of 10 years overall experience in or related to biotechnology or pharmaceutical industry required. Must have experience in maintenance and construction projects and service vendor management. Significant project management skills in all areas of facilities operations. Ability to read and interpret architectural prints, job specifications etc. Knowledge of OSHA guidelines, and other federal, state and local regulations is required. Strong focus on working collegially with team members from across the organization. Pharmaceutical GMP and GLP knowledge preferred. Experience with asset, maintenance, and facilities management systems. Working knowledge of EH&amp;S. Meet the physical requirements of this role including stooping, standing, walking, climbing stairs/ladders, and the ability to lift/carry up to 50 lbs. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is onsite with an office setting based in Bedford, MA. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Engineering & Facilities

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Director of Regulatory Affairs will be responsible for developing and executing global regulatory strategies for product development and approval in alignment with the corporate strategy for Stoke’s portfolio.&nbsp; Manages compounds through all phases of development, including post approval. Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Develop and implement global regulatory strategies and plans for assigned programs. Develop risk assessment plans. Proactively anticipates risks and establishes risk mitigation with team and management. &nbsp; Ensure project team colleagues, line management and key stakeholders are appraised of developments that may impact regulatory success, exercising sound judgement and communicating in a professional and timely manner. Lead regulatory sub-team and represents RA in product development project teams. Continually expand therapeutic area knowledge, and ensures strategic messaging and content of global regulatory dossiers. Lead cross-functional stakeholders and regulatory professionals to ensure inclusion of appropriate and clear strategic messaging in the content of global regulatory dossiers and responses to regulatory Agency requests. Direct point of contact with health authorities, leads and manages FDA meetings. Prepare and deliver effective presentations for external and internal audiences on a variety of drug development topics. Contribute to the continuous improvement of existing processes and strategies, providing recommendations in their area of expertise. Monitors and anticipates industry trends that impact both the regulatory and access environments to strengthen product development plan(s) and adopt regulatory strategies in a timely manner. Required Skills &amp; Experience: 10+ years minimum of Regulatory Affairs experience in the pharmaceutical industry. Rare disease development preferred. BS., M.S. or Ph.D. in a scientific discipline preferred. Strong working knowledge of drug development processes and regulatory requirements. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Strong interpersonal skills and proven ability to effectively manage a variety of senior personnel including medical, scientific, and manufacturing staff. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Regulatory Affairs, Global Regulatory Strategy

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Director/Senior Director, Medical Affairs (Midwest) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary. Location(s): Stoke operates in Bedford, MA.&nbsp; This position is remote, field- based in the continental United States, and is responsible for a regionally designated area. Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Medical Affairs - Midwest

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Reporting into the SVP of Global Medical Affairs, the Executive Director is responsible for providing leadership and strategic direction to a team of US based Regional Medical Directors (RMD) to establish collaborative and scientific engagement with clinical experts to help advance our medicines for patients. Key Responsibilities: Leads and oversees ongoing development of a team of rare disease US Regional Medical Director team that helps advance engagement with Stoke and external credibility of our science. Responsible for overall day-to-day support of all operational and leadership aspects of the RMD team including onboarding, goal setting, coaching and ongoing development of team and individual talent. Work closely with clinical operations and development teams to build and foster collaborative research relationships with academic and community KOLs in support of Stoke clinical studies. Develop and maintain credible, scientific relationships with academic and medical thought leaders. Works closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, staffing medical booths, gathering competitive intelligence, and developing insight reports. Develop, share, and discuss ongoing clinical insights with program team and cross-functional members to ensure awareness and understanding of key opinion leader perspectives to inform and guide strategic decision-making. Work closely with the RMDs to develop and implement strategic territory plans, including KOL engagement plans for top centers of comprehensive care within a defined region. Develop, tracks and represents key impact measures and insights to internal partners to ensure ongoing understanding of strategic alignment and impact of medical affairs activities. Collaborate closely with Sr Director of KoL Engagement and other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Ensure appropriate policies and monitoring are in place to ensure appropriate and compliant team activities. Ability to travel 50%+, including the ability to travel overnight, internationally, and occasionally on weekends. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience: Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Excellent verbal and written communication skills, and exceptional interpersonal skills Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills Valid driver's license and the ability to travel as necessary. Location(s): Stoke is located in Bedford, MA.&nbsp; This position may be a remote or hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 50%+ travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director, Field Medical Affairs

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Key Responsibilities: Executive Director/VP, Commercial Operations works cross-functionally to identify and implement best practices, methodologies and processes to achieve commercial and operational excellence. This role will be responsible for the operational build, development and maintenance of commercial systems, forecasting, market research, field data support, policies and governance documents to support both the launch and commercialization of Stoke’s products. This will include planning for weekly performance reports and insights at launch. The role will also be responsible for field force incentive compensation, working closely with field leadership in Sales and Market Access. Additionally, in partnership with IT, they will identify, develop and deploy enterprise functions and platform technology solutions to maximize operational effectiveness, and work closely with third party vendors such as CRM providers and data aggregators. Work closely within Commercial and Finance to build, analyze and communicate Commercial program budgets to ensure accurate forecasting reporting. Responsible for developing and reporting daily/weekly revenue process with Finance at launch. Collaborate with Finance and Manufacturing to develop and support the monthly/quarterly revenue forecasting process. Collaborate with Commercial stakeholders to coordinate and synergize all market research needs and efforts. Work with cross-functional team members to identify and prioritize actionable, high-impact insights across a variety of core business areas including Sales, Medical Affairs, Marketing, Finance, Market Access, Patient Services and IT. Research, design, implement, and validate cutting-edge dashboards to analyze diverse sources of data to achieve targeted outcomes. Institute processes for data and reporting governance of ad-hoc field-based inquiries from the Executive Leadership Team and other Commercial functions. Design, develop, implement and lead the company's data warehouse with input from Commercial, IT, Quality and Finance. Strategic and tactical planning for field teams including management of vehicle allowance, incentive compensation design and administration, data management, reporting, and CRM management. Lead data inquiry and data stewardship efforts in support of field-based teams Collaborate with IT to develop Stoke’s enterprise data strategy Any and all other responsibilities as requested Required Skills &amp; Experience: A minimum of 10 years of pharmaceutical/biotech industry experience. Bachelor’s degree or equivalent experience in Commercial Operations, Business Intelligence, Data Management or Analytics. Good verbal and written communication with solid organizational, time management, and project management skills. Strong personal leadership skills to drive performance and support corporate objectives. Ability to work collaboratively and effectively with others. Ability to work under pressure and meet deadlines. Strong analytical and communication skills. Well organized and detail oriented. Creativity and resilience in problem solving. Relentless hands-on drive to implement and accomplish performance goals. Effective management of vendor partners to ensure project scope is achieved within defined budget. Ability to independently research, design, and implement platform technology solutions. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director/Vice President, Commercial Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Sr. Director, Medical Information will oversee the development and execution of the medical information strategy, ensuring the provision of accurate, balanced, and timely scientific and clinical information to healthcare professionals, patients, and internal stakeholders. This role will collaborate closely with Clinical Development, Medical Affairs, Advocacy, Regulatory, Pharmacovigilance, and other cross-functional teams to support product knowledge and ensure compliance with industry standards. This position reports into the Executive Director, Global Medical Communications. Key Responsibilities: Lead the Medical Information function, ensuring the development of high-quality medical information responses and resources. Oversee the handling of medical inquiries from healthcare professionals, caregivers, patients, and internal teams, ensuring responses align with company policies and regulations. Develop and maintain medical information databases, standard response documents, and FAQs. Ensure compliance with industry regulations, including FDA, EMA, and other global health authorities. Collaborate with cross-functional teams to provide medical insights that inform product development, medical strategy, and commercial activities. Train internal stakeholders, including Medical Science Liaisons and Sales teams, on medical information policies and best practices. Monitor emerging scientific data and competitor activity to keep medical information materials up-to-date. Develop and implement key performance indicators (KPIs) to assess the effectiveness of medical information services. Represent the company in external medical information forums and industry meetings. Required Skills &amp; Experience: Advanced degree (PharmD, MD, PhD, or equivalent) in a life sciences field. Minimum of 7-10 years of experience in Medical Information or related roles within the pharmaceutical or biotechnology industry. Strong knowledge of global regulatory guidelines governing medical information activities. Proven leadership experience with the ability to develop and manage high-performing teams. Excellent communication and presentation skills, with the ability to translate complex scientific data into understandable content for diverse audiences. Proficiency in medical literature evaluation and database management. Ability to work cross-functionally in a fast-paced, innovative environment. Experience with medical information systems and technology platforms is preferred. Location(s): Stoke operates sites in Bedford, MA.&nbsp; This position is hybrid/remote based position that will require a consistent presence with our office based staff. Travel: This position will require approximately ~10% travel, including occasional presence at internal meetings or conferences as required.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Global Medical Information

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Director, Market Access Strategy &amp; Marketing will lead the development and execution of strategies and materials in support of a potential launch of the first ever disease modifying therapy in Dravet Syndrome, along with future medicines in our pipeline. This includes assessing market dynamics and policies, developing strategies and tactics that communicate the value of our therapy(ies) to key market access stakeholders. You will play a pivotal role in ensuring payer readiness, supporting product reimbursement, and aligning internal teams on access strategy during pre-launch and launch phases. This role will also support strategy and marketing for other functions within the market access department such as Distribution, Field Reimbursement, and Nurse Education. This role is ideal for a resourceful, cross-functional leader who thrives in a start-up environment. Key Responsibilities: Strategy Development Lead the development of the U.S. market access marketing strategy aligned with the broader commercial and brand strategy. Define and communicate the product’s value proposition for payer and organized customer audiences. Contribute to the development of pricing and reimbursement strategy in partnership with Market Access leadership. Tools &amp; Resources Translate scientific and clinical data into compelling, payer-relevant value communications tailored to rare disease decision-makers. Lead development of payer marketing materials, including AMCP dossiers, payer decks, value proposition tools, and digital content. Develop and manage the creation of core market access marketing materials including unbranded and branded payer decks, early engagement tools, and payer advisory content. Plan and oversee future development of AMCP dossiers, payer engagement models, and field access tools. Support early access initiatives and pre-approval information exchange strategies. Develop field market access materials and training to support Account Directors and other market access Field Teams. Manage agency partners effectively and stay on time and within budget Customer Engagement Lead insight generation with US payers to inform access pull-through strategy. Support development and implementation of innovative access programs to optimize coverage, reimbursement, and patient access. Cross-Functional Leadership Work closely with Medical Affairs, Brand Marketing, Legal, and Compliance to ensure alignment and approval of materials. Support launch readiness and post-launch execution. Analytics &amp; Insights Leverage market research, payer insights, and competitive intelligence to refine access strategies and messaging. Monitor access environment trends and assess their implications on brand strategy. Required Skills &amp; Experience:&nbsp; Bachelor’s degree required; advanced degree (MBA, PharmD, MPH, etc.) preferred. 10+ years of experience in biotech/pharmaceutical market access, including at least 5 years in payer/market access marketing. Prior launch experience in specialty therapeutics; rare disease and medical benefit preferred. Deep understanding of U.S. healthcare landscape, payer decision-making, and pricing &amp; reimbursement dynamics. Experience developing market access tools and engaging with internal and external stakeholders. Strong strategic thinking, communication, and project management skills. Comfortable working in a fast-paced, entrepreneurial, and highly collaborative environment. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Market Access Strategy & Marketing

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Director, Regulatory Labeling, Advertising &amp; Promotion will serve as an expert on FDA regulations, guidance, and enforcement trends governing the labeling development and promotion of Stoke’s products. Provides regulatory oversight to ensure regulatory compliance of promotional materials generated for assigned investigational medicinal products and subsequently approved products. Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Responsible for strategy, development, and implementation of labeling as well as systems to manage labeling lifecycle. Provide label strategies to ensure claims can be supported by development. Lead cross-functional Labeling teams to align labeling strategy and content. Develop and maintain the target product profile, the company core datasheet, the patient labeling, the USPI, and the summary of product characteristics or international equivalents. Provide regulatory advice and information to cross-functional teams and to other functional areas (i.e., CMC, nonclinical, and clinical) for product development and planning to support the timely achievement of company and department goals. Interpret regulations and guidance relevant to the development and implementation of labeling. Provide strategic oversight to ensure promotional materials and non-promotional materials generated for external communication regarding commercialized products and/or compounds in development adhere to regulatory compliance standards. Develop systems for material content review and approval. Provide regulatory input and guidance to relevant stakeholders regarding marketing strategies, promotion objectives, and plans. Represent regulatory at promotional review meetings to assist commercial with development of compliant promotional materials. Primary liaison with FDA’s OPDP. Collaborate with Regulatory Affairs, Medical, Legal, Commercial, Ad Agencies, and Compliance to ensure alignment and effective execution of corporate objectives while mitigating potential regulatory risk. Required Skills &amp; Experience: A BS/BA degree or equivalent, and a minimum of 12 years’ experience in Regulatory Affairs specific to Regulatory Affairs Labeling, Advertising and Promotion. Knowledge of applicable regulations and regulatory guidance specific to labeling, advertising and promotion of pharmaceutical/biologic products for healthcare professionals, payor and consumer audiences. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Proven ability to manage multiple complex projects, with the flexibility and adaptability to re-prioritize workload. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Regulatory Labeling Development and Advertising & Promotional

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Sr. Manager, GCLP Compliance, is responsible for overseeing all aspects of compliance for GCLP testing for Stoke’s clinical development programs with Stoke’s Contract Research Organizations (CROs) partners. This person will be a key contributor in growing and shaping Stoke’s GCLP quality monitoring program and will be responsible for supporting Stoke’s DMPK study team towards ensuring data quality and compliance with regulations (e.g., FDA, EMA, etc.). This candidate will be required to contribute to the establishment and maintenance of Stoke’s Quality Management System for the areas of GCP and GCLP. This person will partner with Stoke’s Quality, DMPK and clinical study teams to create a quality culture within Stoke and help reach a sustained state of inspection readiness. This role requires the person to identify and effectively communicate compliance risks and drive action plans to mitigate risks. This role will report into the Director of Clinical Quality and will interface closely with other cross-functional groups such as DMPK, Clinical Development, Clinical Operations, Regulatory and other functions with our vendors and across the Stoke organization. The ideal candidate will have a strong working knowledge of relevant GCP/GCLP FDA, EU, ICH guidelines, and regulations and experience working with CROs and vendors. This candidate should have experience in auditing and a demonstrated ability to communicate significant observations in a sound and factual manner. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for quality management is essential to this role. The candidate should be willing to roll up their sleeves and directly contribute to the implementation of processes and resolution of issues as required. Key Responsibilities: Contribute to the development of procedures to oversee the quality component of Stoke’s GCLP/ Bioanalytical work at the vendors. Provide support with vendor qualification and management. Partner closely with members of the DMPK and Clinical Development Operations teams to support the successful and compliant execution of GCLP bioanalytical work to meet regulatory requirements, guidelines, internal policies, and procedures. Collaborate with Subject Matter Expert’s (SMEs) to develop, maintain and report on metrics from quality monitoring plans. Support internal, in-process, study, and process audits related to GCLP activities and bioanalytical studies. Oversee and assist with audits of vendors for GCLP Bioanalytical laboratories, as necessary, and work with Stoke’ technical SMEs staff to ensure the implementation of appropriate CAPAs as applicable. Support investigations into GCLP non-compliance. Partner with CROs and the DMPK and clinical study team to analyze investigation findings to identify root/probable cause. Ensure adequate investigation, documentation, and implementation of appropriate CAPAs. Assure timely reporting of potential or confirmed violations, as appropriate, to regulatory agencies. Represent QA on project teams, as needed and provide GCLP compliance interpretation, consultation, training, and other supportive services necessary to maintain and improve the quality of research for timely regulatory submissions. Help train/prepare Stoke employees for regulatory inspections. Provide input to and review of responses to regulatory agency questions resulting from regulatory inspections. Contribute to the development of Stoke’s Quality culture. Required Skills &amp; Experience: S. in Biochemistry, Chemistry, Biology, Pharmaceuticals&nbsp;or equivalent with a minimum of 10+ years’ combined experience in a GCP/GLP environment supporting the development of biopharmaceutical products. Experience in RNA based therapeutic modalities preferred. Fully versed in GCP/GLP, ICH Guidelines, FDA, and European regulatory agency requirements (understanding of other regulatory guidelines for other countries a plus). Experience building systems from scratch and scaling processes. Practical knowledge of GCLP related to audits and business standards. Attention to detail with the ability to work within a multi-disciplinary team, as well as with external partners and vendors to establish and maintain a strong quality relationship. Ability to make decisions that are guided by applicable regulatory mandates, Stoke and designated vendor policies, procedures, and business plans. Ability to work autonomously, provide QA advice, effectively manage time, and deliver results on time. Ability to collaborate effectively in a dynamic, cross-functional matrix environment. Strong verbal and written communication skills. Conflict resolution/management and negotiation skills. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, GCLP Compliance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking an enthusiastic and experienced bioinformatician to join the computational group focusing on mRNA splicing and antisense oligonucleotides. Drug discovery at Stoke begins with computational biology. As part of the early discovery team led by Stoke’s co-founder, you will work collaboratively with data and experimental scientists to expand our analytical approaches in identifying therapeutic targets relevant to the TANGO technology. Key Responsibilities: Assist in the development of strategic plans for analyzing and deciphering next-generation sequencing data, and apply modern computational biology approaches to advance Stoke's portfolio of therapeutic targets. Propose and develop analytical and visualization tools to deliver complex biological data. Build, deploy, and maintain bioinformatics applications on Amazon Web Services. Contribute to the development and maintenance of web applications to facilitate target identification and data analysis. Use computational tools to investigate novel approaches to upregulate gene expression. Provide program-specific support for pre-clinical and clinical groups, such as biomarker development and mechanism of action of ASO clinical candidates. Support a highly collaborative environment and promote positive teamwork among a team of scientists from different backgrounds. Communicate scientifically rigorous results clearly, verbally and in writing, both internally and externally. Required Skills &amp; Experience: A degree in bioinformatics, computational biology, computer science, mathematics, systems biology, or any related quantitative field. Previous experience in the biotech or pharma industry; 14 years with a bachelor’s degree, 8 years with a master’s degree, or 4 years with a Ph.D. &nbsp; Experience in and deep understanding of posttranscriptional gene expression regulation, including but not limited to RNA splicing, RNA surveillance. pathways, RNA binding proteins, and antisense oligonucleotides. Fluency in coding languages such as Python and R, including scientific modules and packages commonly used in bioinformatics. Familiarity with machine learning algorithms and applications is a plus. Direct experience in developing and maintaining reproducible analytical workflows for high-throughput sequencing assays such as bulk or single-cell RNA-Seq, eCLIP, or whole-genome sequencing using workflow management systems such as Snakemake or Nextflow. Management experience in leading computational scientists and interns. Location(s):&nbsp;&nbsp; This position is a hybrid position with an office setting based in our Bedford, MA location.&nbsp;&nbsp; Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Scientist, Bioinformatics

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: We are seeking a dedicated and highly motivated Principal Scientist in Oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists in managing the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines. Successful candidates will have significant experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report to the Director, Chemistry. Key Responsibilities: Independently design and implement scientific and synthesis strategies relating to the research and development of antisense and other synthetic oligonucleotide therapeutics. Contribute to multiple projects and provide key technical knowledge to the project team. Plan and execute solid-phase oligonucleotide synthesis for SAR campaigns on several projects. Participate in the identification, development, validation and implementation of both current and next-generation technologies to enable proprietary ASO therapeutic development including purchase and installation of hardware and software supporting synthesis, purification, and characterization of oligonucleotides. Demonstrate working knowledge of conjugation chemistry and techniques aimed at producing novel conjugates. Demonstrates understanding of the chemistry of nucleosides, nucleotides, and oligonucleotides as applied to antisense and other oligonucleotide technologies. Stay current with scientific literature and understanding of the state of the art in oligonucleotide chemistry, purification, and screening strategies. Work with both internal chemistry team and external CRO’s to generate innovative solutions to synthetic and drug design challenges within the context of oligonucleotide drug discovery projects. Authors reports and manuscripts. May contribute to patent filings and/or regulatory filing. &nbsp; Required Skills &amp; Experience: PhD in Oligonucleotide Chemistry and relevant experience in a drug discovery environment with at least 8 years of oligonucleotide synthesis experience in a drug discovery environment, Master’s degree with 12+ years of experience or Bachelor’s degree with 18+ years of experience. Strong practical knowledge of design, synthesis, and purification of synthetic oligonucleotides. Experience in scale-up synthesis would be a plus. Excellent project/time management, and problem-solving skills. Excellent written and verbal communication skills, including making effective and compelling presentations in both large and small groups and communicating complex scientific and nonscientific issues to program teams. Strong people skills and the ability to thrive in a team and goal driven environment Experience designing, synthesizing and purifying oligonucleotides to support research and scale up studies. Proven track record of scientific contributions in oligonucleotide and conjugates research as evidenced by publications/presentations/patent&nbsp;applications and/or the identification of preclinical development candidates. Location(s): Stoke operates in Bedford, MA. This is a lab-based position based in our Bedford, MA location. Travel: This position will require approximately 0% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $169,000 - $191,000 The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Principal Scientist, Oligonucleotide Chemistry

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose:&nbsp; We are seeking experienced Associate Scientist who will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of CNS diseases. This position will report to a Principal Scientist in the in vitro pharmacology group. As part of the group you will work collaboratively to design and perform gene and protein expression assays to support development of new ASO-based therapies. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types. Experience with iPSC-derived CNS cell types is a plus. Evaluate oligonucleotide-based drug candidates in cell-based assays. Conduct in vitro disease model cellular assays to evaluate drug candidates. Design, execute and troubleshoot routine experiments independently. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, qPCR, western blotting and other project-specific assays, as necessary. Familiarity with ELISA and capillary-based protein assays in a plus. Use microscopy and immunohistochemistry-based methods to evaluate cellular phenotypes. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts. Present research findings at group meetings. Work independently as well as collaborate with peers in a result-oriented research environment. Required Skills &amp; Experience:&nbsp; BS with a minimum of 7 years post-graduate experience or M.S. with a minimum of 4 years of experience in neuroscience, biotechnology, cell and molecular biology or related field, preferably in the biotech or pharma industry. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong Hands-on experience in RNA isolation techniques, mammalian cell culture and cell-based assays is strongly desirable. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is an on-site position located in Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, in vitro Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Stoke is seeking an enthusiastic and highly motivated Senior Research Associate to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. Key Responsibilities: Design and execute routine studies to investigate the PK/PD and efficacy of drug candidates. Perform routine to intermediate handling procedures and injections, monitor animal health, and collect tissues in mice. Perform moderately complex behavioral experiments in animal models. Support the implementation of new animal models. Demonstrate technical proficiency to investigate, identify, develop, optimize, and troubleshoot new methods/ techniques/ instrumentation to address critical project needs. Read and understand scientific literature. Analyze data, author technical reports, and document experiments in an electronic notebook. Participate and present at project team and departmental meetings. Maintain laboratory organization including equipment upkeep and ensure compliance with safety protocols. Collaborate across functional areas. Required Skills &amp; Experience: BS with 7+ years post-graduate experience or M.S. with 4+ years of experience working in a biotechnology or pharmaceutical company or contract research organization. Experience working with animal disease models. Experience with animal behavioral assays. Experience with subcutaneous and intravenous injections in rodents; intracerebroventricular injection experience a plus. Experience with molecular biology assays such as PCR, ELISA, MSD, BCA, Western Blot (or JESS). Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Experience in neuroscience a plus. Experience with ISH/IHC a plus. Location(s): This position is based in our Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: we are seeking a dedicated and highly motivated senior research associate in oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists on the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines while reporting to your manager. Successful candidates will have experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report into the Director, Chemistry. Key Responsibilities: The role is primarily responsible for supporting the automated synthesis, cleavage, deprotection, purification, analysis and packaging of oligonucleotides, including heavily modified oligonucleotides. Maintaining laboratory equipment, including the oligonucleotide synthesizer, HPLC machines and benchtop equipment to ensure consistent and reliable oligonucleotide production. Performing critical evaluation and optimization of the processes to improve synthesis yield, purity and timelines for production of modified oligonucleotides Manage ordering and organization of chemicals and supplies necessary for oligonucleotide synthesis Analyzing and interpreting data as well as writing technical reports and protocols, with supervision. Presents research results at lab meetings. Keeps up to date on relevant scientific literature and developments to support ongoing research efforts. Required Skills &amp; Experience: S. with 4 years of experience or M.S. with 2 years of relevant industry experience Hands-on experience with Oligo synthesis (mid and large scale preferred). Experience with MerMade 12 and 192 synthesizers preferred. Experience in preparing buffers for purification and maintaining lab instruments such as AKTA purifiers, UF, HPLC and LC-MS. Previous experience using Microsoft Excel, Word, PowerPoint and Chemdraw Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Research Associate, Oligonucleotide Chemistry

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Stoke Therapeutics is seeking to hire to an Associate Scientist who will support ongoing research efforts in designing and evaluating antisense therapies for diverse targets focusing on CNS and/or Cardiovascular rare diseases. This position will report directly to a Principal Scientist in the in vitro pharmacology group. The ideal candidate will provide hands on laboratory experience to design, implement and execute research assignments for a specific project/projects. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types and cell lines. Experience with culture and differentiation of human iPSC-derived neurons and cardiomyocytes. Perform gene perturbation and pharmacological studies. Evaluate oligonucleotide-based drug candidates in cell-based assays. Conduct in vitro disease model cellular assays to evaluate drug candidates. Design, execute and troubleshoot routine experiments independently. Perform cell-based and molecular assays such as – RNA extraction, qPCR, RT-PCR, protein extraction and specific assays as necessary. Experience and familiarity with ELISA, MSD assay, AlphaLISA assay, western blot and capillary-based protein assays (JESS). Familiarity with plate-based assay development and Nano String is a plus. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts Present research findings at group meetings. Work independently as well as collaborate with peers in a result-oriented research environment. Additional work as assigned Key Requirements:&nbsp; B.S with 7 years post-graduate experience or M.S. with 4 years of experience preferably in Biology, biotech or pharma industry. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong Hands-on experience in RNA isolation techniques, mammalian cell culture and cell-based assays is strongly desirable. Strong hands-on experience in plate-based assays. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): Stoke operates in Bedford, MA. This position is located in Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Associate Director/Director, Clinical Science will serve as a scientific and analytical leader, working cross-functionally to define, plan, and execute key clinical data analyses to support clinical development and publication strategy, regulatory submissions and other integrated activities. The successful candidate will bring a strong foundation in clinical science and data analysis, with a demonstrated ability to translate scientific questions into visual figures and tables. The Associate Director/Director of Clinical Science will be a key liaison between clinical development, biostatistics, data management, medical affairs/publications, communications and regulatory affairs, driving analytical deliverables across multiple studies and programs. This role also includes broader clinical scientist responsibilities including leading strategic clinical development initiatives across multiple indications. This individual will provide key input into clinical development activities that include trial design and development of clinical study documents and communications, focusing on data collection and data interpretation. This position will report into the Senior Director, Clinical Science. Key Responsibilities: Clinical Data Analysis Strategy (Primary Focus): Lead cross-functional efforts to identify clinical data analysis needs across studies and programs Define and drive the clinical data analysis strategy to support clinical decision-making, safety/efficacy evaluation, publications and communications, and regulatory filings Collaborate with biostatistics, and data management to access, structure, and interpret clinical trial datasets and TLFs Perform and/or support exploratory and ad hoc analyses to support internal discussions, publications/communications and regulatory responses Ensure analytical outputs align with scientific objectives and regulatory expectations, maintaining data integrity and consistency across documents Present complex data findings clearly to cross-functional teams and externally as needed Clinical Science Responsibilities: Develop, co-author and review key strategic documents including but not limited to Clinical Development Plans, Protocol Concepts and Full Protocols, Investigator Brochures, Briefing Documents, Safety Updates, IND/NDA submission documents, Responses to Health Authorities Inquires and Clinical Study Reports in conjunction with other members of the clinical study/program teams as needed Serve as an interface for clinical studies between clinical development and multiple internal functions including clinical development operations, data management, biostatistics, regulatory, program management and DMPK/biomarkers and external teams as needed for clinical-development initiated projects Develop expertise in core clinical assessments incorporated in clinical studies and provide education and training to key stakeholders, including at investigator meetings, as needed, in order to ensure quality completion Support medical monitoring activities, including review of eligibility, safety data review and trend identification Collaborate with functions including data management to continuously enhance processes for the timely and efficient internal review of clinical data Develop effective working relationships with key stakeholders including investigators to optimize scientific quality and innovation in clinical study design, execution, reporting and publication Represent clinical development at advisory boards, investigator meetings, site initiations, and other external engagements Attend scientific meetings to interact with investigators and advisors and to present clinical data, as needed Required Skills &amp; Experience: Advanced degree required (e.g., M.S., PharmD, or Ph.D. ) in a life science, clinical research or another similar field At least 7 years of clinical drug development experience Experience in neurology indications preferred and rare disease indications highly preferred Strong knowledge of FDA/EMA/ICH requirements and good clinical practice Strong analytical ability including familiarity with TLFs/statistical outputs and proficiency in data analysis tools (e.g., Prism, RStudio, JMP) Experience having close collaboration with data management, biostatistics/programming teams Exceptional written and oral communication skills tailored to varied audiences Proven ability to work independently and collaboratively, as required, in a dynamic, fast-paced start-up environment consisting of internal and external team members Strong interpersonal skills commensurate with the need to work closely with a broad range of stakeholders, from investigators to internal teams Attention to detail and a commitment to delivering high-quality work Strong planning, organization and time management skills including the ability to prioritize multiple, complex initiatives A sense of passion and urgency to achieve goals and improve the lives of patients Location(s): Stoke operates in Bedford, MA. This position is hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director/Director, Clinical Science- Data Strategy and Analysis

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Research Associate will support ongoing research efforts in designing and evaluating antisense therapies for diverse group of diseases. This position will report to a Senior scientist in the Discovery Biology Group. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types. Screen and evaluate ASOs in relevant mouse tissues and cell-based assays. Perform cell and molecular biology techniques such as RNA, DNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Troubleshoot experiments, analyze and interpret data, participate in development of new assays with support from more senior scientists. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Present research findings at group meetings. Required Skills &amp; Experience:&nbsp; S with 2 years of post-graduate experience in molecular and cell biology research, preferably in industry or a Master’s degree in molecular or cellular biology. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong hands-on experience in RNA isolation techniques from cells and tissues, mammalian cell culture and cell-based assays is strongly desirable. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is onsite in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Research Associate, Biology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Senior Research Associate to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, formulation lead. Key Responsibilities: Support the design and execution of formulation studies for oligonucleotide drug products Perform laboratory experiments in a safe and efficient manner and record research findings in ELN Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional tasks as assigned Required Skills &amp; Experience: B.S. with 4 years of experience or M.S. with 2 years of experience in Chemistry or Organic Chemistry Prior industry experience&nbsp;in oligonucleotide formulation and scale-up is desired Sound knowledge of analytical chemistry and basic nucleic acid chemistry is preferred Experience with UPLC, LCMS and formulation characterization instrument is desired Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Research Associate, Formulation

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: &nbsp; The Senior Manager, CMC Project Management will have responsibility for delivering operational success across the entire development portfolio. This role will support the integrated project management for drug substance, formulation development, analytical development (drug substance and drug product) and packaging &amp; labeling. The candidate will be accountable for team deliverables, ensuring operational success with respect to timelines, and attainment of technical, regulatory and goals/milestones. This person will also be leveraged as a thought partner for the CMC function that works closely with QA and Regulatory CMC to ensure all key stakeholders are aligned with the overall CMC strategy to support the programs. This position will report to the Director, CMC Project Management. Key Responsibilities: Responsible for cross-functional project management across CMC subfunctions (drug substance, drug product, analytical, label &amp; package) and their outsourced manufacturing partners for early phase clinical programs. Plan, manage, and track integrated CMC activities to ensure uninterrupted supply of clinical trial materials; escalate risks as appropriate. Support RFP generation and contract management with outsourced manufacturing partners. Support budget preparation and tracking. Liaison between CMC and other departments (e.g., Quality, Clinical, Regulatory) for early phase clinical programs. Support and/or represent CMC function at program level meetings. Provide project management support as needed for late phase and commercial programs. Coordinate with alliance management personnel for partnered programs as needed. Demonstrate best practices for meeting management and knowledge management. Other duties as assigned. Required Skills &amp; Experience:&nbsp; Bachelor’s/Master’s degree in a discipline relevant to project management, pharmaceutical research, development or manufacturing with relevant industry experience. Professional certification in project management (e.g. CAPM, PMP, PRINCE2, or equivalent) preferred. Minimum of 2 years of experience required in a GMP drug substance or drug product development and manufacturing environment for small molecules, oligonucleotides, polypeptides, or biologics. Direct experience working at CDMOs or in a role with strong CDMO oversight. Experience with Quality requirements for batch release and disposition, as well as general Quality Assurance procedures. Proven success working in cross-functional teams including QA and regulatory CMC. Strong scientific background and knowledge of the drug development process. Experience with Microsoft Project/Smartsheet, Power Point, Excel, Word along with other related software. Experience with preparation of integrated Gantt charts using OnePager, Office Timeline, or equivalent preferred. Experience in managing multiple programs at one time, can work well with different management styles to keep a project moving. Competency in developing, implementing, and delivering CMC project plans (milestones, timelines, resources, etc.) to successful endpoints. Strong conflict management and negotiation skills. Superb written and oral communication skills. Experience with oligonucleotides is a plus. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 10% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, CMC Project Management

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Scientist I will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of diseases. This position will report to a Principal Scientist in the in vitro Group. Key Responsibilities: Primarily responsible for designing, executing, implementing, and troubleshooting routine studies in collaboration with cross functional groups. Independently design and execute molecular assays to identify preclinical drug candidates targeting disease genes. Develop high-quality cell-based assays to inform disease target gene engagement. Devise and test new strategies and technologies to modify gene expression in collaboration with colleagues. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Work with CRO laboratories and external collaborators to support internal studies. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts and patents with assistance. Present research findings at group, cross-functional team and lab meetings. Acts as a technical resource within own group/project team and collaborates with other interdisciplinary functions. Required Skills &amp; Experience:&nbsp; BS with 10 years post-graduate experience, M.S. with 6 years of experience, or a Ph.D. in cell or molecular biology or a related field. Experience in molecular biology, RNA techniques, and cell-based assays are a must. Previous work experience in anti-sense oligonucleotide-based target validation. Experience in cell culture techniques with variety of cells (previous work experience in the culture and differentiation of human iPSC to neuronal cell types would be beneficial). Work experience in therapeutic areas involving CNS or ocular disease highly desirable. Strong organizational and recordkeeping skills, experience using ELN is highly desirable. Strong oral and written communication skills. Location(s): This position is lab based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I, in vitro Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Scientist I will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of diseases. This position will report to a Principal Scientist in the Discovery Biology Group. Key Responsibilities: Primarily responsible for designing, executing, implementing, and troubleshooting routine studies in collaboration with cross functional groups. Independently design and execute molecular assays to identify preclinical drug candidates targeting disease genes. Develop high-quality cell-based assays to inform disease target gene engagement. Devise and test new strategies and technologies to modify gene expression in collaboration with colleagues. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Work with CRO laboratories and external collaborators to support internal studies. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts and patents with assistance. Present research findings at group, cross-functional team and lab meetings. Acts as a technical resource within own group/project team and collaborates with other interdisciplinary functions. Required Skills &amp; Experience:&nbsp; S with 10 years post-graduate experience, M.S. with 6 years of experience, or a Ph.D. in cell or molecular biology or a related field. Experience in molecular biology, RNA techniques, and cell-based assays are a must. Previous work experience in anti-sense oligonucleotide-based target validation. Experience in cell culture techniques with variety of cells (previous work experience in the culture and differentiation of human iPSC to neuronal cell types would be beneficial). Work experience in therapeutic areas involving CNS or ocular disease highly desirable. Strong organizational and recordkeeping skills, experience using ELN is highly desirable Strong oral and written communication skills. Location(s):&nbsp;&nbsp; Stoke is located in Bedford, MA. This position is onsite with an office setting based in Bedford, MA. Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly experienced and strategic Director/Senior Director of Statistical Programmer to lead and grow our statistical programming function. This role will be instrumental in supporting our expanding clinical development pipeline, including a pivotal phase 3 trial. The ideal candidate will bring deep technical expertise, leadership experience, and collaborative mindset to help drive successful regulatory submissions and clinical trial execution. The Director/Senior Director will have proven leadership ability in a fast paced, environment and interact with all levels of internal staff as well as external stakeholders, including managing external consultants and CROs. This position will report into the Executive Director, Head of Biostatistics. Key Responsibilities: Provide strategic oversight for statistical programming activities across multiple development programs. Serve as the statistical programmer functional lead for the phase 3 pivotal trial, ensure robust data and analysis workflows to support NDA/MAA submission, including integrated summaries (ISE/ISS), working closely with regulatory, biostatistics, and clinical data management teams Oversee and perform quality control (QC) review of datasets, tables, listing and figures (TLFs) generated by CROs and vendors to ensure accuracy, consistency, and regulatory compliance. Ensure adherence to CDISC standards (SDTM, ADaM, Define-XML) and regulatory agency requirements. Guide programming support for exploratory and ad-hoc analyses to generate clinical and scientific insights. Establish and implement processes to ensure quality control, traceability, and reproducibility of analyses. Develop and maintain programming standards, SOPs, and QC procedures that align with regulatory expectations. Manage vendor relationships to ensure deliverables are on time, high-quality, and audit-ready. Partner cross-functionally to support clinical study reports (CSRs), data monitoring committees, and regulatory interactions. Provide leadership in planning for future in-house statistical programming capabilities, including team building and process development as needed. Why Join Us: Opportunity to build the statistical programming function in a growing biotech. Direct involvement in advancing multiple drug candidates, including a pivotal phase 3 program. Small, agile team environment with visibility and impact at the highest levels of the company. Mission-driven organization focused on developing disease-modifying therapies for patients with high unmet medical need. Required Skills &amp; Experience: Master’s degree (or higher) in statistics, Computer Science, Mathematics, or related field. 10+ years statistical programming experience in biotech industry. Proven track record leading programming for pivotal phase 3 trials and regulatory submissions (NDA/MAA/BLA). Hands-on expertise in SAS and R programming, with strong knowledge of CDISC SDTM, ADaM, Define-XML, and FDA submission standards. Experience managing external vendor/CROs in a global clinical development environment. Experience with machine learning programming is a plus. Knowledge of AI/ML is a plus. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Statistical Programmer

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Formulation Scientist to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, Formulation Lead. Key Responsibilities: Design and execute formulation studies for oligonucleotide drug products Prepare prototype formulations and conduct pre-formulation characterization (e.g., solubility, pH stability, buffer selection) Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Maintain accurate and timely documentation of experiments, protocols, and results in electronic lab notebooks Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional duties as assigned Required Skills &amp; Experience: PhD in Pharmaceutical Sciences, Chemistry, Biochemistry, or related field, Master’s degree with 6+ years of experience in formulation development, or Bachelor’s with 10+ years of experience Experience with nucleic acid-based therapeutics preferred Familiarity with oligonucleotide properties, degradation pathways, and formulation challenges Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I, Formulation

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Scientist will work collaboratively across the Research organization to support preclinical efforts in understanding the target engagement, pharmacology, and efficacy of novel compounds in disease models. This position requires a scientist with experience designing and executing thoughtful in vivo studies and developing assays to establish and investigate preclinical biomarkers in tissue samples. This position will report into the Executive Director of in vivo pharmacology. Key Responsibilities: Support the in vivo characterization of novel compounds by designing and overseeing in life studies and performing molecular analysis with a combination of internal and outsourced resources. Work collaboratively across functions to support programs through preclinical proof of concept and candidate selection to advance the company pipeline. Use a high level of scientific judgment to adapt and/or create standard methods and techniques by applying breadth of knowledge and prior work experience. Develop and optimize preclinical biomarker assays to support discovery efforts and bridge into early development activities. Maintain excellent and timely recordkeeping while adhering to aggressive timelines Author study reports that may be used for regulatory filings and scientific manuscripts. Required Skills &amp; Experience: Bachelor’s degree with 16+ years of experience, or Master’s degree with 10+ years of post-graduate experience, or Ph.D. with 6+ (industry experience is a plus). Degrees in Pharmacology, Neuroscience or relevant disciplines highly preferred. Experience working in drug discovery with an emphasis on neurology indications, experience working with oligonucleotides is a plus. Designing and managing experiments at contract research organizations. Experience developing and optimizing assays in tissue matrix (i.e MSD, ELISA, qPCR), IHC skills a plus. Excellent verbal and written communication skills. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): Stoke operates in Bedford, MA.&nbsp; This position is onsite with an office based in Bedford, MA. Travel: This position will require approximately 5% travel.&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Vice President, Global Medical Affairs, will be an individual contributor role with key strategic leadership responsibilities to inform and guide clinical, medical affairs and commercial work across genetic developmental and epileptic encephalopathy (DEE) indications. This individual will serve as a medical expert, building and maintaining strong relationships with internal functions and with external key opinion leaders, healthcare professionals, and patient advocacy groups. They will collaborate actively with clinical development and biostatistics in the design of clinical studies and actively guide analysis and interpretation of data to ensure the safe and effective use of our therapies, generating real-world evidence, and contributing to the overall success of our mission. This position will report into SVP Global Medical Affairs. Key Responsibilities: Collaborate closely with Medical Affairs Field Medical team to establish and maintain strong scientific relationships and collaboration with key experts and investigators in pediatric developmental and epileptic encephalopathies. Plan and execute ongoing advisory and strategic working groups to gather expert advice to guide strategic decision-making and medical affairs work. Collaborate closely with clinical development to engage with clinical experts to guide clinical study design and execution of clinical trials. Represent Stoke in presentation of clinical data at scientific conferences, medical meetings, and other external events. Provide medical expertise in close collaboration with clinical development, biostats, HEOR and medical affairs to critically interpret clinical data and define product profile. Assist medical communications and investigators in the development and publication of important findings to advance clinical and scientific understanding in defined areas. Provide medical expertise and support to internal stakeholders, including commercial, clinical development, regulatory, and market access teams. Contribute to the development of medical communication and education materials, including scientific abstracts, manuscripts, and slide decks. Provide expertise in delivering medical training to internal staff in support of onboarding and ongoing education. Facilitate interdisciplinary collaboration to enhance patient experience, including working with non-physician providers, nurses, pharmacists, office staff, and therapists. Ensure compliance with all relevant regulatory guidelines and ethical standards in medical affairs activities. Monitor and analyze the competitive landscape and emerging scientific trends in pediatric epilepsy. Required Skills &amp; Experience: MD/DO degree required. Board certification in Neurology, Pediatrics, or a related specialty is highly preferred. Minimum of 10-15 years of combined clinical and pharmaceutical or biotechnology industry, with a significant focus on pediatric epilepsy or rare neurological diseases. Deep clinical experience and expertise in pediatric epilepsy is essential. Excellent communication, presentation, and interpersonal skills, with the ability to effectively engage with diverse stakeholders. Demonstrated ability to build and maintain strong relationships with key opinion leaders and external experts. Thorough understanding of regulatory requirements and ethical guidelines governing medical affairs activities. Ability to travel globally as required. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is remote position. Travel: This position will require approximately 50-70% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Global Medical Affairs - Pediatric Neurology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The ED, ZOR Marketing will lead development of pre-commercial marketing activities, including evolution of a recently-launched disease awareness campaign and efforts to enhance genetic testing in Dravet Syndrome. The incumbent will also be responsible for developing a launch strategy and tactics in Dravet Syndrome.&nbsp; This position will report to the Vice President of Marketing. Key Responsibilities: Develop and manage impactful brand and marketing communications. programs, which support key initiatives and market development efforts. Plan and implement campaigns which integrate all appropriate marketing tactics, including digital, interactive and online media, mobile applications, and other new methods that augment traditional marketing tactics. Identify and turn physician/brand insights into actionable programs that have a beneficial impact on the growth of the business. Support key business functions across the business (e.g. DTC campaign, patient access programs, speaker bureau etc.). Creates the execution of a Global Marketing Strategy as well as action plans for sales force aligned activities. Proactively collaborate and strategize with Commercial teams to ensure alignment and develop regionally relevant, robust marketing and promotional programs. Develop ZOR positioning, messaging platform and corporate brand architecture. Oversee and manage creative agencies. Manage and approve expenditures within budget guidelines. Communicate brand and marketing strategy to the organization, ensuring consistency across all customer touch points. Scope and incorporate appropriate voice of customer research and insights into campaign development and decision-making process. Develop and champion Stoke’s brand to ensure it is consistently and meaningfully represented by all internal stakeholders, across all customer touch points. Develop and/or evolve department operational procedures and processes that foster effective communications. Establish metrics and track program effectiveness and ROI.&nbsp; Required Skills &amp; Experience: Bachelor's degree required. Master’s degree preferred. Minimum of 12 years of experience required, with 5+ years of marketing executive experience in the pharmaceutical, biotech, life science, medical device or general healthcare industries. Solid acumen around all functions that are integral to successfully evaluating potential opportunities (R&amp;D, regulatory, commercial, financial, manufacturing, legal, etc.). Experience with rare disease product launch. Proven experience managing complex marketing operations processes (Promotional Material Review, Brand Planning, Market Research, CRM Management etc.). Familiarity with marketing automation principles and marketing automation platforms. Strong understanding of related aspects of marketing concepts and principles; patient and consumer marketing. Possess the ability and desire to measure and track performance and success of programs/initiatives. Excellent interpersonal relationship skills including negotiating and relationship management skills with ability to drive achievement of objectives. Ability to manage competing priorities in a fast-paced environment. Experience in managing external agencies and vendors and managing budgets. Proven successful project management skills; strict attention to detail. Drive and enthusiasm for operational improvements. Ability to work in an ambiguous environment and successful track record in building brand teams from the ground up. Excellent communication and people skills. Successful experience in a fast-paced entrepreneurial environment. Marketing and management experience at the VP level Strength in managing people leaders.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a remote position with an office setting based in Bedford, MA location. Travel: This position will require approximately 30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $242,000 - $308,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director, Zorevunersen Marketing

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Medical Director, Neurology Clinical Development will play a key role in the planning, execution, and interpretation of clinical studies focused on transformative therapies for life-threatening neurological diseases. This individual will contribute medical and scientific expertise to advance programs from early development through late-stage studies and regulatory submission. The Associate Medical Director will support cross-functional teams, interface with external partners and investigators, and help ensure the highest standards of scientific and clinical rigor are maintained across all programs. This role will work collaboratively with the Medical Director and Senior Medical Director in Neurology Clinical Development and will report to the Medical Director in Neurology Clinical Development. Key Responsibilities: Clinical Development &amp; Execution Support the design, implementation, and oversight of clinical trials in neurology, including protocol development, study conduct, and data analysis Contribute to the medical and scientific content of key clinical documents (e.g. protocols, investigator brochures, clinical study reports, and regulatory submissions) Participate in clinical data review, interpretation, and presentation to support timely and informed decision-making Collaborate with safety/pharmacovigilance on safety review and signal evaluation Provide medical monitoring and clinical oversight for ongoing studies under the supervision of the Medical Director or Senior Medical Director Maintain effective collaboration with CROs, investigators, and academic partners to ensure study quality and adherence to timelines Ensure compliance with GCP, ICH, and all applicable regulatory and ethical guidelines Scientific &amp; Strategic Contribution Support the development of clinical development strategies and study designs in alignment with program objectives and scientific rationale Contribute to the development, execution, and continuous refinement of global Clinical Development Plans (CDPs) Integrate emerging data, literature, and competitive intelligence to inform ongoing program decisions and ensure scientific relevance and rigor Collaborate closely with cross-functional partners including regulatory, clinical development operations, and biostatistics to ensure consistency and accuracy in study execution Participate in internal discussions and presentations to communicate key scientific insights, data interpretations, and development updates Contribute to the preparation and review of strategic and regulatory documents, including target product profiles, clinical sections of IND/CTA submissions, and briefing packages External Engagement &amp; Communication Engage and collaborate with key opinion leaders (KOLs) and academic investigators to inform study designs and strengthen the scientific foundation of development programs Represent the clinical development organization at scientific and medical meetings, advisory boards, and key investigator interactions Contribute to the authorship of abstracts, manuscripts, and scientific presentations that communicate program progress and data to the broader scientific and clinical community Leadership &amp; Collaboration Provide clinical and scientific leadership with cross-functional clinical teams, fostering an environment of accountability, curiosity, and collaborative problem solving Partner effectively with internal stakeholders across clinical development operations, biostatistics, regulatory, and commercial to ensure scientific and operational excellence in study execution Serve as a clinical development point of contact in collaborations and partnerships, helping to maintain strong relationships built on scientific integrity, transparency, and shared objectives Required Skills &amp; Experience: MD, MD/PhD, or PharmD candidates with relevant clinical development experience in neurology, rare/orphan, or genetic diseases Board certified or eligible in neurology, pediatrics, or a relevant specialty (for MDs) Minimum 5 years experience contributing to the design, conduct, or medical oversight of clinical trials (industry or academic) required Familiarity with FDA/EMA regulations and ICH/GCP guidelines Strong scientific, analytical, and organizational skills Demonstrated ability to interpret complex clinical and scientific data Excellent communication and presentation skills, with the ability to collaborate effectively across multidisciplinary teams High personal integrity, scientific rigor, and strong commitment to advancing therapies for patients with serious neurological diseases Location(s): Stoke operates in Bedford, MA. This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 20% travel (domestic and international). Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $200,000 - $235,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Medical Director, Neurology Clinical Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking an enthusiastic and highly motivated Associate Scientist to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. Key Responsibilities: Design and execute routine studies to investigate the PK/PD and efficacy of drug candidates. Perform routine to intermediate handling procedures and injections, monitor animal health, and collect tissues in mice. Perform moderately complex behavioral experiments in animal models. Support the implementation of new animal models. Demonstrate technical proficiency to investigate, identify, develop, optimize, and troubleshoot new methods/ techniques/ instrumentation to address critical project needs. Read and understand scientific literature. Analyze data, author technical reports, and document experiments in an electronic notebook. Participate and present at project team and departmental meetings. Maintain laboratory organization including equipment upkeep and ensure compliance with safety protocols. Collaborate across functional areas. Required Skills &amp; Experience: BS with 7+ years post-graduate experience or M.S. with 4+ years of experience working in a biotechnology or pharmaceutical company or contract research organization. Experience working with animal disease models. Experience with animal behavioral assays. Experience with subcutaneous and intravenous injections in rodents; intracerebroventricular injection experience a plus. Experience with molecular biology assays such as PCR, ELISA, MSD, BCA, Western Blot (or JESS). Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Experience in neuroscience a plus. Experience with ISH/IHC a plus. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $114,000 - $138,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director, Patient Advocacy &amp; Engagement will build upon and execute strategic advocacy engagement initiatives for Stoke’s Dravet syndrome, ADOA, and Syngap1 programs, ensuring alignment across key Stoke functions throughout the product lifecycle. There will be a special focus on launch readiness in the U.S. for zorevunersen (Dravet syndrome) and capacity building in ADOA and Syngap1. Reporting to the Vice President of Patient Advocacy and Policy, the candidate must have strong experience in biotechnology or pharmaceutical advocacy with a broad understanding of the business. This person must thrive in a fast-paced environment, be motivated by the patient need and operate with utmost integrity. The Director will work collaboratively with cross functional team members to increase awareness of our programs in the patient community, and bring the patient voice into the everyday decision-making, programming and employee experience. Key Responsibilities: Serve as a liaison between the company and patient community by maintaining strong, trusted relationships, proactively requesting and sharing insights, and responding to community questions and needs in a timely fashion Ensure a strong connection between employees and the patient community, including identifying innovative and compliant ways for employees to connect with patients, families and disease communities Collaborate internally and play a contributing role on cross-functional teams to create and deliver patient-facing programs and materials (i.e. clinical trial awareness, launch preparedness) Ensure timely and accurate communication of company information and updates to the appropriate patient communities Partner effectively with Product &amp; Corporate Communications, Medical Affairs and Clinical Operations to ensure timely, consistent and compliant communication and responses to external patient inquiries about our programs, including clinical trials and access Coordinate with Medical Affairs and other internal functions to review and approve patient organization funding and collaboration requests; actively contribute to policy and process design/improvements for these and other related activities Lead initiatives to ensure patient insights are brought into Stoke decision-making such as advisory boards and ongoing input into programming and messaging In collaboration with Medical Affairs, organize Stoke’s strategic engagement with patient advocacy organizations at congresses and events Support broader Corporate Affairs efforts, including employee engagement and corporate communications Develop and deliver on an annual plan aligned to corporate and department goals, including developing and tracking KPIs and metrics to measure the success of advocacy initiatives; continually innovate and elevate Stoke’s approach to patient advocacy and engagement by continuously learning and staying current with best practices Manage the patient advocacy budget and lead and oversee external partners, consultants and agency resources. Required Skills &amp; Experience:&nbsp; Bachelor’s degree, preferably in social science, science, public policy, or a health-related field 10 years of professional experience in patient advocacy in a publicly traded biotech/pharmaceutical company or health-related non-profit.&nbsp;The ideal candidate has experience in rare disease, regulatory milestones (i.e., FDA Advisory Committee meetings and approvals), KOL and Society engagement, and commercial-stage experience A thorough understanding of the pharmaceutical product lifecycle, including the pre-clinical, clinical, regulatory processes and commercial stages Established track record of building and maintaining strong, trusted relationships with colleagues as well as with non-profit and/or patient communities Demonstrated ability to create and lead successful patient advocate programs and partnerships in collaboration with internal teams and stakeholders Excellent communication skills: written, verbal and presentation delivery; A good listener Strategic thinker and problem solver; intellectually curious and perceptive; able to identify interdependencies and connections across the business; a critical thinker Comfortable in a fast-moving,&nbsp;rapidly changing&nbsp;environment; able to establish structure and process when needed; consistently prioritizes high-impact projects; calm under pressure; works with a sense of purpose and urgency Understanding of laws and regulations governing the biopharmaceutical industry&nbsp;with an understanding of regulatory bodies that impact communications and reimbursement, understanding of publicly-traded company communications requirements and rules Proven understanding of the needs of individuals living with severe and rare diseases and a demonstrated passion for supporting the patient community Holds self and others to a high standard of ethics and integrity. Maintains confidentiality, exercises good judgment and discretion Strong leadership skills with demonstrated ability to influence and collaborate across the organization and at all levels, both internally and externally Able to&nbsp;work&nbsp;independently with minimal supervision Experience working with all levels of management and consulting with key business stakeholders with an ability to influence for greater outcomes Ability to travel to key U.S. and global congresses and patient events, as needed (up to 30%) Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position (2-3 days/week) with an office setting based in Bedford, MA location. Travel: This position will require approximately 30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $231,000 - $242,000 The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Patient Advocacy & Engagement

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a dedicated and highly motivated Principal Scientist in Oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists in managing the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines. Successful candidates will have significant experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report to the Director, Chemistry. Key Responsibilities: Independently design and implement scientific and synthesis strategies relating to the research and development of antisense and other synthetic oligonucleotide therapeutics. Contribute to multiple projects and provide key technical knowledge to the project team. Plan and execute solid-phase oligonucleotide synthesis for SAR campaigns on several projects. Participate in the identification, development, validation and implementation of both current and next-generation technologies to enable proprietary ASO therapeutic development including purchase and installation of hardware and software supporting synthesis, purification, and characterization of oligonucleotides. Demonstrate working knowledge of conjugation chemistry and techniques aimed at producing novel conjugates. Demonstrates understanding of the chemistry of nucleosides, nucleotides, and oligonucleotides as applied to antisense and other oligonucleotide technologies. Stay current with scientific literature and understanding of the state of the art in oligonucleotide chemistry, purification, and screening strategies. Work with both internal chemistry team and external CRO’s to generate innovative solutions to synthetic and drug design challenges within the context of oligonucleotide drug discovery projects. Authors reports and manuscripts. May contribute to patent filings and/or regulatory filing. &nbsp; Required Skills &amp; Experience: PhD in Oligonucleotide Chemistry and relevant experience in a drug discovery environment with at least 8 years of oligonucleotide synthesis experience in a drug discovery environment, Master’s degree with 12+ years of experience or Bachelor’s degree with 18+ years of experience. Strong practical knowledge of design, synthesis, and purification of synthetic oligonucleotides. Experience in scale-up synthesis would be a plus. Excellent project/time management, and problem-solving skills. Excellent written and verbal communication skills, including making effective and compelling presentations in both large and small groups and communicating complex scientific and nonscientific issues to program teams. Strong people skills and the ability to thrive in a team and goal driven environment Experience designing, synthesizing and purifying oligonucleotides to support research and scale up studies. Proven track record of scientific contributions in oligonucleotide and conjugates research as evidenced by publications/presentations/patent&nbsp;applications and/or the identification of preclinical development candidates. Location(s): Stoke operates in Bedford, MA. This is a lab-based position based in our Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose:&nbsp; The Associate Director will be responsible for preparation of preclinical safety data packages for clinical development. The ideal candidate will have extensive experience with the design, execution and interpretation of in vitro and in vivo toxicology studies. The candidate will be responsible for coordinating and interfacing with CROs to undertake these studies, and will be accountable for the review, analysis and reporting of the safety pharmacology and toxicology data. This individual will need to work across a multi-disciplinary team to advance programs through the pipeline. The successful candidate will have extensive experience of reviewing and creating regulatory documents (INDs, CTAs, NDAs etc.) and interacting with global regulatory agencies. This position will report to the Executive Director, Head of Toxicology.&nbsp; Key Responsibilities:&nbsp; Provide leadership, technical oversight, and guidance for all nonclinical safety studies conducted to support discovery and development programs and ensure delivery of a high-quality safety package for development.&nbsp; Design and manage nonclinical safety studies, including the evaluation, interpretation an integration of safety pharmacology, toxicology and toxicokinetic information.&nbsp; Manage the preparation and presentation of nonclinical safety data for project teams, regulatory agencies, and management.&nbsp; Prepare toxicology sections in regulatory documents (IND, CTA, IB and annual reports) and aid in preparation of responses to regulatory agencies.&nbsp; Develop an optimal CRO network to support non-clinical safety studies and manage external CROs to conduct appropriate studies.&nbsp; Manage toxicology goals, priorities, resource requirements, budget, and timelines.&nbsp; Collaborate with Discovery Research, DMPK, Chemistry/CMC functions as well as with key Clinical and Regulatory stakeholders.&nbsp; Regularly assess the oligonucleotide drug development field and use best practice for safety studies.&nbsp; Represent Stoke Therapeutics toxicology with key external stakeholders, partners, and consultants.&nbsp; &nbsp; Required Skills &amp; Experience:&nbsp;&nbsp; M.S. similar in Pharmacology, Toxicology, or a relevant discipline and 12+ years of experience or PhD and 8+ years of experience in Toxicology or a relevant field&nbsp; Track record supporting drug discovery programs from target identification through development with small molecules and/or oligonucleotides&nbsp; Experience in all aspects of toxicology including general, developmental and reproductive toxicology, carcinogenicity, toxicokinetics, and safety pharmacology&nbsp; Knowledge of FDA GLP and ICH guidelines&nbsp; Experience authoring regulatory documents and interacting with FDA and ideally, EU regulatory agencies.&nbsp; Effective management of external collaborations and service providers (CROs) and ability to bring pragmatic business minded approach to balancing internal and external capabilities.&nbsp; Familiarity with oligonucleotides or messenger RNA therapeutics&nbsp; Experience with drug development in the CNS and/or eye is a plus&nbsp; Strong organizational and recordkeeping skills.&nbsp; Strong presentational skills to be able to communicate to both internal and external audiences.&nbsp; Highly motivated cross-functional team player with the ability to work in a fast-paced environment. Ability to provide novel, innovative solutions to challenging problems.&nbsp;&nbsp;&nbsp;&nbsp; Location(s):&nbsp;&nbsp; Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office based in Bedford, MA location.&nbsp;&nbsp; Travel:&nbsp; This position will require approximately 10% travel.&nbsp;&nbsp; Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $189,000 - $258,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, Toxicology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Senior Research Associate to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, formulation lead. Key Responsibilities: Support the design and execution of formulation studies for oligonucleotide drug products Perform laboratory experiments in a safe and efficient manner and record research findings in ELN Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional tasks as assigned Required Skills &amp; Experience: B.S. with 4 years of experience or M.S. with 2 years of experience in Chemistry or Organic Chemistry Prior industry experience&nbsp;in oligonucleotide formulation and scale-up is desired Sound knowledge of analytical chemistry and basic nucleic acid chemistry is preferred Experience with UPLC, LCMS and formulation characterization instrument is desired Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $99,000 - $108,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Vice President (VP) of Clinical Operations is a strategic executive responsible for providing leadership, oversight, and direction for all global clinical trial operations. Reporting to the Senior Vice President (SVP), Clinical Development Operations, the VP will drive the successful, timely, and cost-effective execution of the clinical trial portfolio. This role leads a high-performing Clinical Operations organization and partners closely with other functions to ensure studies are conducted in accordance with Good Clinical Practice (GCP), regulatory requirements, and the company’s strategic goals. The VP will also serve as the deputy for the SVP, Clinical Development Operations, providing executive-level support, representation, and continuity of leadership as needed. Key Responsibilities: Provide operational leadership for Clinical Operations across all programs, from study planning and start-up through execution and close-out. Oversee selection, performance and governance of CROs, external vendors and service partners, ensuring consistent alignment with company standards, timelines, quality expectations, and budgets. Partner cross-functionally with leaders across Clinical Development Operations, Regulatory Affairs, Medical, Biostatistics, Medical Affairs, and other key functions to drive integrated program delivery. Provide oversight of the framework for defining, reviewing, and acting on key performance indicators (KPIs) and risk management plans, ensuring proactive issue escalation and alignment between Clinical Operations, Clinical Outsourcing, and Quality. Build, mentor, and empower a high-performing Clinical Operations organization, fostering accountability and professional growth. Lead or contribute to regulatory submission strategies (e.g., NDA, BLA, MAA) and provide operational leadership during inspections and health authority interactions. Champion a culture of quality, operational excellence, and collaboration across the organization. Oversee the implementation of best practices and industry standards in clinical operations, maintaining up-to-date SOPs and continuous process improvement initiatives. Support the SVP, Clinical Development Operations in strategic planning, governance meetings, and executive decision-making; act as deputy when required. Required Skills &amp; Experience: Bachelor’s or advanced degree in Life Sciences or a related field, with approximately 20 years of progressive experience in Clinical Operations, including at least 3 years leading a Clinical Operations department. Prior experience in rare disease, pediatric, gene therapy, or other high-complexity indications preferred. Demonstrated success in delivering complex global clinical programs across multiple therapeutic areas and phases. Experience building or scaling the clinical operations function from early-stage to late-stage programs in a biotech setting. Familiarity with global trial execution across US, EU, Japan, emerging markets and with multi-region regulatory submission strategies. Experience implementing clinical operations systems (CTMS), process improvements and change management. Direct experience contributing to regulatory submissions and marketing authorization applications (e.g., NDA, BLA, MAA, or equivalent). Experience with regulatory inspections (FDA, EMA, PMDA, or other authorities), including preparation and post-inspection follow-up. Deep understanding of ICH GCP, FDA, EMA, and global regulatory requirements. Strong vendor/CRO oversight, budget management, and strategic execution expertise. Exceptional communication, leadership presence, and strategic decision-making skills. Ability to thrive in a fast-paced, dynamic, and highly collaborative environment. Location(s): Stoke operates sites in Bedford, MA. This position is hybrid/remote based position that will require a consistent presence in our office at least 3 days a week. Travel: This position will require approximately 15% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $311,000 - $374,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Clinical Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Reporting to SVP of Translational DMPK and Clinical Pharmacology, this leadership role will be responsible for providing strategic and scientific leadership for DMPK and biomarker discovery and development to support the programs from early discovery through clinical development. Key Responsibilities: To ensure that DMPK/ADME and biomarker data inform key decisions to support regulatory submissions. Drive the DMPK strategy for new programs and support the functional leads for INDs/CTAs. Oversee related authorship and review of key regulatory documents and engagement with global health authorities. Provide thought leadership and decision-making support on IND readiness. Collaborate with clinical development team and author and review the relevant sections of IBs, clinical study protocols, CSRs and NDAs/BLAs. Contribute on and provide leadership to discovery-stage project teams as a subject matter expert, providing PK and PK/PD input to the design of preclinical studies and predict clinical outcomes. Collaborate with R&amp;D team and toxicologist to guide dose selection in preclinical studies, dose/exposure rationale, and safety margins. Ensure that appropriate guidance from regulatory agencies is utilized to support GxP work overseen by DMPK team. Direct in vitro and in vivo DMPK studies, to enable and inform clinical modeling and translational assessments. Lead the integration of biomarker strategies into preclinical and clinical programs to inform drug response, PK/PD modeling and potentially patient stratification. Lead integration of bioanalysis, toxicokinetics, and potentially biomarker strategy into nonclinical programs to inform clinical development and regulatory submissions. Collaborate with the SMEs and mentor scientists in DMPK team. Identify new and manage existing consultants to support DMPK studies. Maintain or develop expertise in evolving clinical pharmacology and pharmacometrics approaches. Assist in forecasting resources and the department budget. Represent the company in external scientific forums, collaborations, and regulatory interactions. Excellent communication skills, including the ability to present complex scientific concepts and make actionable recommendations to the team. Required Skills &amp; Experience: PhD in a scientific discipline with 18+ years of work experience in academia and industry, that is related to drug discovery and development. In depth understanding of DMPK and proven track record of leading DMPK strategies. Background in developing and implementing biomarker strategies to inform drug development across preclinical and clinical stages is essential. Established in leadership role overseeing teams and representing the function to both internal stakeholders. Demonstrated track record of advancing oligonucleotide therapeutics-based programs from early discovery through clinical development and regulatory filings. Knowledge of FDA/EMA/ICH guidelines to support GxP work in DMPK. Experience in overseeing CROs, external consultants, and working with cross-functional program teams. Strong leadership, team building, and communication skills; ability to guide functions and levels. Experience in working with therapies targeting neurology is desirable.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $330,000 - $365,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, DMPK & Biomarker Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. Position Purpose: The Executive Director, Clinical Development Operations Excellence and Compliance, is a senior operational leader responsible for elevating the efficiency, quality, and compliance of clinical development operations execution. This role focuses on optimizing operational performance, embedding compliance by design, and ensuring global consistency in processes, systems, and governance frameworks across Clinical Development Operations and Clinical Development. This role will drive operational excellence, department risk mitigation, and continuous process improvement to ensure clinical programs are delivered with speed, quality, and regulatory integrity. Operational Excellence is about embedding an innovative strategy to set Stoke on a path of continuous improvement.&nbsp; It is a focused long-term change and involves every member of the Clinical Development Operations organization, at every level. This position will report into the Senior Vice President, Clinical Development Operations and will be part of the department’s leadership team. Key Responsibilities: Partner with functional leaders to refine and optimize processes that elevate operational excellence and compliance across clinical trials, ensuring efficiency, consistency, and high-quality delivery. Establish and maintain harmonized, scalable processes and other solutions that improve executional quality and cycle time. Integrate regulatory and GCP requirements into operational practices to ensure proactive compliance and inspection readiness. Identify, prioritize, and lead initiatives that strengthen operational capability and organizational learning across clinical development operations. Collaborate with Clinical Development, Regulatory, Quality, and other applicable functions to align processes, mitigate operational risk, and support portfolio execution. Build/connect interface between SOP/process owners and functional leaders across Clinical Development Operations and Clinical Development as needed. Champion digital innovation, process simplification, and change management to modernize how clinical trials are delivered. Participate in governance committees related to operational excellence, clinical compliance, and risk management. Oversee internal audit readiness for Clinical Development Operations and support inspection management across global trials; support Clinical Study Teams with proactive risk mitigation and CAPA implementation as needed. Implement and lead an oversight and monitoring strategy to ensure consistent visibility into clinical execution quality, operational risk, and compliance performance across programs. Establish and lead a Clinical Development Operations Compliance Committee with managers and key study leads to review study quality, deviations, and regulatory compliance. Required Skills &amp; Experience: Degree in Life Sciences, or a related field with 18+ years of experience in Clinical Development Operations or Clinical Operations, with at least 10 years in a senior operational or compliance leadership role. Deep understanding of global clinical research regulations (ICH-GCP, FDA, EMA, PMDA, etc.). Proven experience driving operational excellence and quality improvement initiatives in a complex, matrixed environment. Strong track record in inspection readiness, audit management, and remediation planning. Exceptional leadership, communication, and stakeholder management skills. Demonstrated ability to influence at the executive level and drive cross-functional alignment. Location(s): Stoke is in Bedford, MA.&nbsp; This hybrid position is based at Stoke’s Bedford, MA office, with a minimum on-site requirement of two days per week. Travel: This position will require approximately 25% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $286,000 - $322,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP). Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

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