Stoke Therapeutics is a biotechnology company that focuses on developing RNA-targeted medicines to treat severe genetic diseases. Founded in 2014, Stoke Therapeutics utilizes its proprietary technology platform to address the underlying causes of a range of genetic disorders. The company's innovative approach has the potential to transform the treatment landscape for these conditions, offering new hope to patients and their families.

Research and Development Specialist

Sr. Scientist, Formulation and GMP Manufacturing

Research Scientist in Neurology

Pharmacology Expert

In Vivo Research Specialist

Senior Scientist, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly skilled and motivated Senior Analytical Scientist to join our Analytical and Formulation Sciences team. The ideal candidate will have strong hands-on experience in UPLC method development, and mass spectrometry-based characterization of oligonucleotide and conjugates. This role is critical to supporting the development of innovative oligonucleotide-based therapeutics. This position will report into the Director, CMC. Key Responsibilities: Develop, optimize, and validate UPLC methods for the analysis of oligonucleotides and their conjugates. Conduct mass spectrometry (LC-MS, HRMS, MS/MS) analyses to characterize oligonucleotide and its conjugates. Interpret analytical data to support product development, stability studies, and release testing. Collaborate with cross-functional teams including Process Development, Quality, and Regulatory Affairs. Author technical reports, method development summaries, and regulatory submissions (e.g., IND, BLA). Perform routine formulation preparation and characterization of oligonucleotide drug products for preclinical studies. Required Skills &amp; Experience: Ph.D. in Analytical Chemistry, Biochemistry, Pharmaceutical Sciences, or a related field with 6+ years of relevant industry experience; or M.S. with 10+ years of experience; or Bachelor’s with 16+ years of experience. Proven experience in UPLC method development for oligonucleotides or nucleic acid-based therapeutics. Strong expertise with mass spectrometry for structural and quantitative analysis of oligonucleotide and conjugates is desired. Strong data analysis skills and proficiency with analytical software (e.g., Empower, MassLynx, Xcalibur). Excellent communication, documentation, and organizational skills. Ability to work independently and collaboratively in a fast-paced environment. Knowledge of ICH guidelines and regulatory expectations for analytical method validation. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is based in Bedford, MA. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Analytical Scientist

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a Director, Engineering &amp; Facilities to manage the ongoing operations and labs within our current headquarters in Bedford. Importantly, this person will also oversee and manage alongside the Chief Financial Officer the ongoing search for a potential new headquarters for Stoke. Therefore, this role is critical not only to develop an efficient, safe, and scalable environment across both office and lab settings, but also an opportunity to play an important role in Stoke’s potential move into our new home. It's a hands-on, strategic opportunity for someone who thrives in a fast-paced environment. This position will report into the Chief Financial Officer. Key Responsibilities: Help manage the process for Stoke’s potential new headquarters search. Project manage construction of new offices, if applicable. Oversee technical aspects of new lab construction, including ensuring sufficient lab space and understanding building requirements. Oversee day-to-day operations of the facilities and the functioning of all building systems. Support the design, construction, startup and operation of all locations. Supervise external real estate, architectural, engineering and contractor relationships. Proactively engage with internal cross-functional teams to achieve site goals and ensure the facility is operating smoothly and in compliance with all health and safety standards. Develop and formalize approval of documents related to site operations including SOPs, safety training, policies and procedures. Ensure building operations comply with all local building codes, zoning laws and regulations. Establish and maintain relationships and contracts with external vendors for services such as ULS inventory management, Airgas, FedEx, and hazardous waste disposal. Review fixed asset inventory and update as required. Maintain and improve site compliance related to environment, health, and safety. Develop and implement processes necessary to operate the facility, including non-GMP cleaning/housekeeping, badging/security, and pest control. Make recommendations to promote efficiencies and apply a proactive approach to potential operational/environmental hazards/risks. Manage projects in support of space planning and expansion across all locations to meet hiring requirements. Develop and maintain budgets and long-range facilities plans based on company growth and future needs. Additional responsibilities as assigned. Required Skills &amp; Experience: Bachelor's degree in engineering or related discipline preferred or equivalent combination of education and work-related experience. Minimum of 10 years overall experience in or related to biotechnology or pharmaceutical industry required. Must have experience in maintenance and construction projects and service vendor management. Significant project management skills in all areas of facilities operations. Ability to read and interpret architectural prints, job specifications etc. Knowledge of OSHA guidelines, and other federal, state and local regulations is required. Strong focus on working collegially with team members from across the organization. Pharmaceutical GMP and GLP knowledge preferred. Experience with asset, maintenance, and facilities management systems. Working knowledge of EH&amp;S. Meet the physical requirements of this role including stooping, standing, walking, climbing stairs/ladders, and the ability to lift/carry up to 50 lbs. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is onsite with an office setting based in Bedford, MA. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Engineering & Facilities

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Director of Regulatory Affairs will be responsible for developing and executing global regulatory strategies for product development and approval in alignment with the corporate strategy for Stoke’s portfolio.&nbsp; Manages compounds through all phases of development, including post approval. Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Develop and implement global regulatory strategies and plans for assigned programs. Develop risk assessment plans. Proactively anticipates risks and establishes risk mitigation with team and management. &nbsp; Ensure project team colleagues, line management and key stakeholders are appraised of developments that may impact regulatory success, exercising sound judgement and communicating in a professional and timely manner. Lead regulatory sub-team and represents RA in product development project teams. Continually expand therapeutic area knowledge, and ensures strategic messaging and content of global regulatory dossiers. Lead cross-functional stakeholders and regulatory professionals to ensure inclusion of appropriate and clear strategic messaging in the content of global regulatory dossiers and responses to regulatory Agency requests. Direct point of contact with health authorities, leads and manages FDA meetings. Prepare and deliver effective presentations for external and internal audiences on a variety of drug development topics. Contribute to the continuous improvement of existing processes and strategies, providing recommendations in their area of expertise. Monitors and anticipates industry trends that impact both the regulatory and access environments to strengthen product development plan(s) and adopt regulatory strategies in a timely manner. Required Skills &amp; Experience: 10+ years minimum of Regulatory Affairs experience in the pharmaceutical industry. Rare disease development preferred. BS., M.S. or Ph.D. in a scientific discipline preferred. Strong working knowledge of drug development processes and regulatory requirements. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Strong interpersonal skills and proven ability to effectively manage a variety of senior personnel including medical, scientific, and manufacturing staff. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Regulatory Affairs, Global Regulatory Strategy

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Director/Senior Director, Medical Affairs (Midwest) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary. Location(s): Stoke operates in Bedford, MA.&nbsp; This position is remote, field- based in the continental United States, and is responsible for a regionally designated area. Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Medical Affairs - Midwest

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Reporting into the SVP of Global Medical Affairs, the Executive Director is responsible for providing leadership and strategic direction to a team of US based Regional Medical Directors (RMD) to establish collaborative and scientific engagement with clinical experts to help advance our medicines for patients. Key Responsibilities: Leads and oversees ongoing development of a team of rare disease US Regional Medical Director team that helps advance engagement with Stoke and external credibility of our science. Responsible for overall day-to-day support of all operational and leadership aspects of the RMD team including onboarding, goal setting, coaching and ongoing development of team and individual talent. Work closely with clinical operations and development teams to build and foster collaborative research relationships with academic and community KOLs in support of Stoke clinical studies. Develop and maintain credible, scientific relationships with academic and medical thought leaders. Works closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, staffing medical booths, gathering competitive intelligence, and developing insight reports. Develop, share, and discuss ongoing clinical insights with program team and cross-functional members to ensure awareness and understanding of key opinion leader perspectives to inform and guide strategic decision-making. Work closely with the RMDs to develop and implement strategic territory plans, including KOL engagement plans for top centers of comprehensive care within a defined region. Develop, tracks and represents key impact measures and insights to internal partners to ensure ongoing understanding of strategic alignment and impact of medical affairs activities. Collaborate closely with Sr Director of KoL Engagement and other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Ensure appropriate policies and monitoring are in place to ensure appropriate and compliant team activities. Ability to travel 50%+, including the ability to travel overnight, internationally, and occasionally on weekends. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience: Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Excellent verbal and written communication skills, and exceptional interpersonal skills Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills Valid driver's license and the ability to travel as necessary. Location(s): Stoke is located in Bedford, MA.&nbsp; This position may be a remote or hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 50%+ travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director, Field Medical Affairs

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Key Responsibilities: Executive Director/VP, Commercial Operations works cross-functionally to identify and implement best practices, methodologies and processes to achieve commercial and operational excellence. This role will be responsible for the operational build, development and maintenance of commercial systems, forecasting, market research, field data support, policies and governance documents to support both the launch and commercialization of Stoke’s products. This will include planning for weekly performance reports and insights at launch. The role will also be responsible for field force incentive compensation, working closely with field leadership in Sales and Market Access. Additionally, in partnership with IT, they will identify, develop and deploy enterprise functions and platform technology solutions to maximize operational effectiveness, and work closely with third party vendors such as CRM providers and data aggregators. Work closely within Commercial and Finance to build, analyze and communicate Commercial program budgets to ensure accurate forecasting reporting. Responsible for developing and reporting daily/weekly revenue process with Finance at launch. Collaborate with Finance and Manufacturing to develop and support the monthly/quarterly revenue forecasting process. Collaborate with Commercial stakeholders to coordinate and synergize all market research needs and efforts. Work with cross-functional team members to identify and prioritize actionable, high-impact insights across a variety of core business areas including Sales, Medical Affairs, Marketing, Finance, Market Access, Patient Services and IT. Research, design, implement, and validate cutting-edge dashboards to analyze diverse sources of data to achieve targeted outcomes. Institute processes for data and reporting governance of ad-hoc field-based inquiries from the Executive Leadership Team and other Commercial functions. Design, develop, implement and lead the company's data warehouse with input from Commercial, IT, Quality and Finance. Strategic and tactical planning for field teams including management of vehicle allowance, incentive compensation design and administration, data management, reporting, and CRM management. Lead data inquiry and data stewardship efforts in support of field-based teams Collaborate with IT to develop Stoke’s enterprise data strategy Any and all other responsibilities as requested Required Skills &amp; Experience: A minimum of 10 years of pharmaceutical/biotech industry experience. Bachelor’s degree or equivalent experience in Commercial Operations, Business Intelligence, Data Management or Analytics. Good verbal and written communication with solid organizational, time management, and project management skills. Strong personal leadership skills to drive performance and support corporate objectives. Ability to work collaboratively and effectively with others. Ability to work under pressure and meet deadlines. Strong analytical and communication skills. Well organized and detail oriented. Creativity and resilience in problem solving. Relentless hands-on drive to implement and accomplish performance goals. Effective management of vendor partners to ensure project scope is achieved within defined budget. Ability to independently research, design, and implement platform technology solutions. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director/Vice President, Commercial Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Sr. Director, Medical Information will oversee the development and execution of the medical information strategy, ensuring the provision of accurate, balanced, and timely scientific and clinical information to healthcare professionals, patients, and internal stakeholders. This role will collaborate closely with Clinical Development, Medical Affairs, Advocacy, Regulatory, Pharmacovigilance, and other cross-functional teams to support product knowledge and ensure compliance with industry standards. This position reports into the Executive Director, Global Medical Communications. Key Responsibilities: Lead the Medical Information function, ensuring the development of high-quality medical information responses and resources. Oversee the handling of medical inquiries from healthcare professionals, caregivers, patients, and internal teams, ensuring responses align with company policies and regulations. Develop and maintain medical information databases, standard response documents, and FAQs. Ensure compliance with industry regulations, including FDA, EMA, and other global health authorities. Collaborate with cross-functional teams to provide medical insights that inform product development, medical strategy, and commercial activities. Train internal stakeholders, including Medical Science Liaisons and Sales teams, on medical information policies and best practices. Monitor emerging scientific data and competitor activity to keep medical information materials up-to-date. Develop and implement key performance indicators (KPIs) to assess the effectiveness of medical information services. Represent the company in external medical information forums and industry meetings. Required Skills &amp; Experience: Advanced degree (PharmD, MD, PhD, or equivalent) in a life sciences field. Minimum of 7-10 years of experience in Medical Information or related roles within the pharmaceutical or biotechnology industry. Strong knowledge of global regulatory guidelines governing medical information activities. Proven leadership experience with the ability to develop and manage high-performing teams. Excellent communication and presentation skills, with the ability to translate complex scientific data into understandable content for diverse audiences. Proficiency in medical literature evaluation and database management. Ability to work cross-functionally in a fast-paced, innovative environment. Experience with medical information systems and technology platforms is preferred. Location(s): Stoke operates sites in Bedford, MA.&nbsp; This position is hybrid/remote based position that will require a consistent presence with our office based staff. Travel: This position will require approximately ~10% travel, including occasional presence at internal meetings or conferences as required.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Global Medical Information

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Director, Market Access Strategy &amp; Marketing will lead the development and execution of strategies and materials in support of a potential launch of the first ever disease modifying therapy in Dravet Syndrome, along with future medicines in our pipeline. This includes assessing market dynamics and policies, developing strategies and tactics that communicate the value of our therapy(ies) to key market access stakeholders. You will play a pivotal role in ensuring payer readiness, supporting product reimbursement, and aligning internal teams on access strategy during pre-launch and launch phases. This role will also support strategy and marketing for other functions within the market access department such as Distribution, Field Reimbursement, and Nurse Education. This role is ideal for a resourceful, cross-functional leader who thrives in a start-up environment. Key Responsibilities: Strategy Development Lead the development of the U.S. market access marketing strategy aligned with the broader commercial and brand strategy. Define and communicate the product’s value proposition for payer and organized customer audiences. Contribute to the development of pricing and reimbursement strategy in partnership with Market Access leadership. Tools &amp; Resources Translate scientific and clinical data into compelling, payer-relevant value communications tailored to rare disease decision-makers. Lead development of payer marketing materials, including AMCP dossiers, payer decks, value proposition tools, and digital content. Develop and manage the creation of core market access marketing materials including unbranded and branded payer decks, early engagement tools, and payer advisory content. Plan and oversee future development of AMCP dossiers, payer engagement models, and field access tools. Support early access initiatives and pre-approval information exchange strategies. Develop field market access materials and training to support Account Directors and other market access Field Teams. Manage agency partners effectively and stay on time and within budget Customer Engagement Lead insight generation with US payers to inform access pull-through strategy. Support development and implementation of innovative access programs to optimize coverage, reimbursement, and patient access. Cross-Functional Leadership Work closely with Medical Affairs, Brand Marketing, Legal, and Compliance to ensure alignment and approval of materials. Support launch readiness and post-launch execution. Analytics &amp; Insights Leverage market research, payer insights, and competitive intelligence to refine access strategies and messaging. Monitor access environment trends and assess their implications on brand strategy. Required Skills &amp; Experience:&nbsp; Bachelor’s degree required; advanced degree (MBA, PharmD, MPH, etc.) preferred. 10+ years of experience in biotech/pharmaceutical market access, including at least 5 years in payer/market access marketing. Prior launch experience in specialty therapeutics; rare disease and medical benefit preferred. Deep understanding of U.S. healthcare landscape, payer decision-making, and pricing &amp; reimbursement dynamics. Experience developing market access tools and engaging with internal and external stakeholders. Strong strategic thinking, communication, and project management skills. Comfortable working in a fast-paced, entrepreneurial, and highly collaborative environment. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Market Access Strategy & Marketing

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Director, Regulatory Labeling, Advertising &amp; Promotion will serve as an expert on FDA regulations, guidance, and enforcement trends governing the labeling development and promotion of Stoke’s products. Provides regulatory oversight to ensure regulatory compliance of promotional materials generated for assigned investigational medicinal products and subsequently approved products. Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Responsible for strategy, development, and implementation of labeling as well as systems to manage labeling lifecycle. Provide label strategies to ensure claims can be supported by development. Lead cross-functional Labeling teams to align labeling strategy and content. Develop and maintain the target product profile, the company core datasheet, the patient labeling, the USPI, and the summary of product characteristics or international equivalents. Provide regulatory advice and information to cross-functional teams and to other functional areas (i.e., CMC, nonclinical, and clinical) for product development and planning to support the timely achievement of company and department goals. Interpret regulations and guidance relevant to the development and implementation of labeling. Provide strategic oversight to ensure promotional materials and non-promotional materials generated for external communication regarding commercialized products and/or compounds in development adhere to regulatory compliance standards. Develop systems for material content review and approval. Provide regulatory input and guidance to relevant stakeholders regarding marketing strategies, promotion objectives, and plans. Represent regulatory at promotional review meetings to assist commercial with development of compliant promotional materials. Primary liaison with FDA’s OPDP. Collaborate with Regulatory Affairs, Medical, Legal, Commercial, Ad Agencies, and Compliance to ensure alignment and effective execution of corporate objectives while mitigating potential regulatory risk. Required Skills &amp; Experience: A BS/BA degree or equivalent, and a minimum of 12 years’ experience in Regulatory Affairs specific to Regulatory Affairs Labeling, Advertising and Promotion. Knowledge of applicable regulations and regulatory guidance specific to labeling, advertising and promotion of pharmaceutical/biologic products for healthcare professionals, payor and consumer audiences. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Proven ability to manage multiple complex projects, with the flexibility and adaptability to re-prioritize workload. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Regulatory Labeling Development and Advertising & Promotional

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Sr. Manager, GCLP Compliance, is responsible for overseeing all aspects of compliance for GCLP testing for Stoke’s clinical development programs with Stoke’s Contract Research Organizations (CROs) partners. This person will be a key contributor in growing and shaping Stoke’s GCLP quality monitoring program and will be responsible for supporting Stoke’s DMPK study team towards ensuring data quality and compliance with regulations (e.g., FDA, EMA, etc.). This candidate will be required to contribute to the establishment and maintenance of Stoke’s Quality Management System for the areas of GCP and GCLP. This person will partner with Stoke’s Quality, DMPK and clinical study teams to create a quality culture within Stoke and help reach a sustained state of inspection readiness. This role requires the person to identify and effectively communicate compliance risks and drive action plans to mitigate risks. This role will report into the Director of Clinical Quality and will interface closely with other cross-functional groups such as DMPK, Clinical Development, Clinical Operations, Regulatory and other functions with our vendors and across the Stoke organization. The ideal candidate will have a strong working knowledge of relevant GCP/GCLP FDA, EU, ICH guidelines, and regulations and experience working with CROs and vendors. This candidate should have experience in auditing and a demonstrated ability to communicate significant observations in a sound and factual manner. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for quality management is essential to this role. The candidate should be willing to roll up their sleeves and directly contribute to the implementation of processes and resolution of issues as required. Key Responsibilities: Contribute to the development of procedures to oversee the quality component of Stoke’s GCLP/ Bioanalytical work at the vendors. Provide support with vendor qualification and management. Partner closely with members of the DMPK and Clinical Development Operations teams to support the successful and compliant execution of GCLP bioanalytical work to meet regulatory requirements, guidelines, internal policies, and procedures. Collaborate with Subject Matter Expert’s (SMEs) to develop, maintain and report on metrics from quality monitoring plans. Support internal, in-process, study, and process audits related to GCLP activities and bioanalytical studies. Oversee and assist with audits of vendors for GCLP Bioanalytical laboratories, as necessary, and work with Stoke’ technical SMEs staff to ensure the implementation of appropriate CAPAs as applicable. Support investigations into GCLP non-compliance. Partner with CROs and the DMPK and clinical study team to analyze investigation findings to identify root/probable cause. Ensure adequate investigation, documentation, and implementation of appropriate CAPAs. Assure timely reporting of potential or confirmed violations, as appropriate, to regulatory agencies. Represent QA on project teams, as needed and provide GCLP compliance interpretation, consultation, training, and other supportive services necessary to maintain and improve the quality of research for timely regulatory submissions. Help train/prepare Stoke employees for regulatory inspections. Provide input to and review of responses to regulatory agency questions resulting from regulatory inspections. Contribute to the development of Stoke’s Quality culture. Required Skills &amp; Experience: S. in Biochemistry, Chemistry, Biology, Pharmaceuticals&nbsp;or equivalent with a minimum of 10+ years’ combined experience in a GCP/GLP environment supporting the development of biopharmaceutical products. Experience in RNA based therapeutic modalities preferred. Fully versed in GCP/GLP, ICH Guidelines, FDA, and European regulatory agency requirements (understanding of other regulatory guidelines for other countries a plus). Experience building systems from scratch and scaling processes. Practical knowledge of GCLP related to audits and business standards. Attention to detail with the ability to work within a multi-disciplinary team, as well as with external partners and vendors to establish and maintain a strong quality relationship. Ability to make decisions that are guided by applicable regulatory mandates, Stoke and designated vendor policies, procedures, and business plans. Ability to work autonomously, provide QA advice, effectively manage time, and deliver results on time. Ability to collaborate effectively in a dynamic, cross-functional matrix environment. Strong verbal and written communication skills. Conflict resolution/management and negotiation skills. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, GCLP Compliance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking an enthusiastic and experienced bioinformatician to join the computational group focusing on mRNA splicing and antisense oligonucleotides. Drug discovery at Stoke begins with computational biology. As part of the early discovery team led by Stoke’s co-founder, you will work collaboratively with data and experimental scientists to expand our analytical approaches in identifying therapeutic targets relevant to the TANGO technology. Key Responsibilities: Assist in the development of strategic plans for analyzing and deciphering next-generation sequencing data, and apply modern computational biology approaches to advance Stoke's portfolio of therapeutic targets. Propose and develop analytical and visualization tools to deliver complex biological data. Build, deploy, and maintain bioinformatics applications on Amazon Web Services. Contribute to the development and maintenance of web applications to facilitate target identification and data analysis. Use computational tools to investigate novel approaches to upregulate gene expression. Provide program-specific support for pre-clinical and clinical groups, such as biomarker development and mechanism of action of ASO clinical candidates. Support a highly collaborative environment and promote positive teamwork among a team of scientists from different backgrounds. Communicate scientifically rigorous results clearly, verbally and in writing, both internally and externally. Required Skills &amp; Experience: A degree in bioinformatics, computational biology, computer science, mathematics, systems biology, or any related quantitative field. Previous experience in the biotech or pharma industry; 14 years with a bachelor’s degree, 8 years with a master’s degree, or 4 years with a Ph.D. &nbsp; Experience in and deep understanding of posttranscriptional gene expression regulation, including but not limited to RNA splicing, RNA surveillance. pathways, RNA binding proteins, and antisense oligonucleotides. Fluency in coding languages such as Python and R, including scientific modules and packages commonly used in bioinformatics. Familiarity with machine learning algorithms and applications is a plus. Direct experience in developing and maintaining reproducible analytical workflows for high-throughput sequencing assays such as bulk or single-cell RNA-Seq, eCLIP, or whole-genome sequencing using workflow management systems such as Snakemake or Nextflow. Management experience in leading computational scientists and interns. Location(s):&nbsp;&nbsp; This position is a hybrid position with an office setting based in our Bedford, MA location.&nbsp;&nbsp; Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Scientist, Bioinformatics

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose:&nbsp; We are seeking experienced Associate Scientist who will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of CNS diseases. This position will report to a Principal Scientist in the in vitro pharmacology group. As part of the group you will work collaboratively to design and perform gene and protein expression assays to support development of new ASO-based therapies. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types. Experience with iPSC-derived CNS cell types is a plus. Evaluate oligonucleotide-based drug candidates in cell-based assays. Conduct in vitro disease model cellular assays to evaluate drug candidates. Design, execute and troubleshoot routine experiments independently. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, qPCR, western blotting and other project-specific assays, as necessary. Familiarity with ELISA and capillary-based protein assays in a plus. Use microscopy and immunohistochemistry-based methods to evaluate cellular phenotypes. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts. Present research findings at group meetings. Work independently as well as collaborate with peers in a result-oriented research environment. Required Skills &amp; Experience:&nbsp; BS with a minimum of 7 years post-graduate experience or M.S. with a minimum of 4 years of experience in neuroscience, biotechnology, cell and molecular biology or related field, preferably in the biotech or pharma industry. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong Hands-on experience in RNA isolation techniques, mammalian cell culture and cell-based assays is strongly desirable. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is an on-site position located in Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, in vitro Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Stoke is seeking an enthusiastic and highly motivated Senior Research Associate to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. Key Responsibilities: Design and execute routine studies to investigate the PK/PD and efficacy of drug candidates. Perform routine to intermediate handling procedures and injections, monitor animal health, and collect tissues in mice. Perform moderately complex behavioral experiments in animal models. Support the implementation of new animal models. Demonstrate technical proficiency to investigate, identify, develop, optimize, and troubleshoot new methods/ techniques/ instrumentation to address critical project needs. Read and understand scientific literature. Analyze data, author technical reports, and document experiments in an electronic notebook. Participate and present at project team and departmental meetings. Maintain laboratory organization including equipment upkeep and ensure compliance with safety protocols. Collaborate across functional areas. Required Skills &amp; Experience: BS with 7+ years post-graduate experience or M.S. with 4+ years of experience working in a biotechnology or pharmaceutical company or contract research organization. Experience working with animal disease models. Experience with animal behavioral assays. Experience with subcutaneous and intravenous injections in rodents; intracerebroventricular injection experience a plus. Experience with molecular biology assays such as PCR, ELISA, MSD, BCA, Western Blot (or JESS). Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Experience in neuroscience a plus. Experience with ISH/IHC a plus. Location(s): This position is based in our Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Associate Director/Director, Clinical Science will serve as a scientific and analytical leader, working cross-functionally to define, plan, and execute key clinical data analyses to support clinical development and publication strategy, regulatory submissions and other integrated activities. The successful candidate will bring a strong foundation in clinical science and data analysis, with a demonstrated ability to translate scientific questions into visual figures and tables. The Associate Director/Director of Clinical Science will be a key liaison between clinical development, biostatistics, data management, medical affairs/publications, communications and regulatory affairs, driving analytical deliverables across multiple studies and programs. This role also includes broader clinical scientist responsibilities including leading strategic clinical development initiatives across multiple indications. This individual will provide key input into clinical development activities that include trial design and development of clinical study documents and communications, focusing on data collection and data interpretation. This position will report into the Senior Director, Clinical Science. Key Responsibilities: Clinical Data Analysis Strategy (Primary Focus): Lead cross-functional efforts to identify clinical data analysis needs across studies and programs Define and drive the clinical data analysis strategy to support clinical decision-making, safety/efficacy evaluation, publications and communications, and regulatory filings Collaborate with biostatistics, and data management to access, structure, and interpret clinical trial datasets and TLFs Perform and/or support exploratory and ad hoc analyses to support internal discussions, publications/communications and regulatory responses Ensure analytical outputs align with scientific objectives and regulatory expectations, maintaining data integrity and consistency across documents Present complex data findings clearly to cross-functional teams and externally as needed Clinical Science Responsibilities: Develop, co-author and review key strategic documents including but not limited to Clinical Development Plans, Protocol Concepts and Full Protocols, Investigator Brochures, Briefing Documents, Safety Updates, IND/NDA submission documents, Responses to Health Authorities Inquires and Clinical Study Reports in conjunction with other members of the clinical study/program teams as needed Serve as an interface for clinical studies between clinical development and multiple internal functions including clinical development operations, data management, biostatistics, regulatory, program management and DMPK/biomarkers and external teams as needed for clinical-development initiated projects Develop expertise in core clinical assessments incorporated in clinical studies and provide education and training to key stakeholders, including at investigator meetings, as needed, in order to ensure quality completion Support medical monitoring activities, including review of eligibility, safety data review and trend identification Collaborate with functions including data management to continuously enhance processes for the timely and efficient internal review of clinical data Develop effective working relationships with key stakeholders including investigators to optimize scientific quality and innovation in clinical study design, execution, reporting and publication Represent clinical development at advisory boards, investigator meetings, site initiations, and other external engagements Attend scientific meetings to interact with investigators and advisors and to present clinical data, as needed Required Skills &amp; Experience: Advanced degree required (e.g., M.S., PharmD, or Ph.D. ) in a life science, clinical research or another similar field At least 7 years of clinical drug development experience Experience in neurology indications preferred and rare disease indications highly preferred Strong knowledge of FDA/EMA/ICH requirements and good clinical practice Strong analytical ability including familiarity with TLFs/statistical outputs and proficiency in data analysis tools (e.g., Prism, RStudio, JMP) Experience having close collaboration with data management, biostatistics/programming teams Exceptional written and oral communication skills tailored to varied audiences Proven ability to work independently and collaboratively, as required, in a dynamic, fast-paced start-up environment consisting of internal and external team members Strong interpersonal skills commensurate with the need to work closely with a broad range of stakeholders, from investigators to internal teams Attention to detail and a commitment to delivering high-quality work Strong planning, organization and time management skills including the ability to prioritize multiple, complex initiatives A sense of passion and urgency to achieve goals and improve the lives of patients Location(s): Stoke operates in Bedford, MA. This position is hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director/Director, Clinical Science- Data Strategy and Analysis

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a dedicated and highly motivated Principal Scientist in Oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists in managing the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines. Successful candidates will have significant experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report to the Director, Chemistry. Key Responsibilities: Independently design and implement scientific and synthesis strategies relating to the research and development of antisense and other synthetic oligonucleotide therapeutics. Contribute to multiple projects and provide key technical knowledge to the project team. Plan and execute solid-phase oligonucleotide synthesis for SAR campaigns on several projects. Participate in the identification, development, validation and implementation of both current and next-generation technologies to enable proprietary ASO therapeutic development including purchase and installation of hardware and software supporting synthesis, purification, and characterization of oligonucleotides. Demonstrate working knowledge of conjugation chemistry and techniques aimed at producing novel conjugates. Demonstrates understanding of the chemistry of nucleosides, nucleotides, and oligonucleotides as applied to antisense and other oligonucleotide technologies. Stay current with scientific literature and understanding of the state of the art in oligonucleotide chemistry, purification, and screening strategies. Work with both internal chemistry team and external CRO’s to generate innovative solutions to synthetic and drug design challenges within the context of oligonucleotide drug discovery projects. Authors reports and manuscripts. May contribute to patent filings and/or regulatory filing. &nbsp; Required Skills &amp; Experience: PhD in Oligonucleotide Chemistry and relevant experience in a drug discovery environment with at least 8 years of oligonucleotide synthesis experience in a drug discovery environment, Master’s degree with 12+ years of experience or Bachelor’s degree with 18+ years of experience. Strong practical knowledge of design, synthesis, and purification of synthetic oligonucleotides. Experience in scale-up synthesis would be a plus. Excellent project/time management, and problem-solving skills. Excellent written and verbal communication skills, including making effective and compelling presentations in both large and small groups and communicating complex scientific and nonscientific issues to program teams. Strong people skills and the ability to thrive in a team and goal driven environment Experience designing, synthesizing and purifying oligonucleotides to support research and scale up studies. Proven track record of scientific contributions in oligonucleotide and conjugates research as evidenced by publications/presentations/patent&nbsp;applications and/or the identification of preclinical development candidates. Location(s): Stoke operates in Bedford, MA. This is a lab-based position based in our Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Principal Scientist, Oligonucleotide Chemistry

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Scientist I will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of diseases. This position will report to a Principal Scientist in the Discovery Biology Group. Key Responsibilities: Primarily responsible for designing, executing, implementing, and troubleshooting routine studies in collaboration with cross functional groups. Independently design and execute molecular assays to identify preclinical drug candidates targeting disease genes. Develop high-quality cell-based assays to inform disease target gene engagement. Devise and test new strategies and technologies to modify gene expression in collaboration with colleagues. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Work with CRO laboratories and external collaborators to support internal studies. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts and patents with assistance. Present research findings at group, cross-functional team and lab meetings. Acts as a technical resource within own group/project team and collaborates with other interdisciplinary functions. Required Skills &amp; Experience:&nbsp; S with 10 years post-graduate experience, M.S. with 6 years of experience, or a Ph.D. in cell or molecular biology or a related field. Experience in molecular biology, RNA techniques, and cell-based assays are a must. Previous work experience in anti-sense oligonucleotide-based target validation. Experience in cell culture techniques with variety of cells (previous work experience in the culture and differentiation of human iPSC to neuronal cell types would be beneficial). Work experience in therapeutic areas involving CNS or ocular disease highly desirable. Strong organizational and recordkeeping skills, experience using ELN is highly desirable Strong oral and written communication skills. Location(s):&nbsp;&nbsp; Stoke is located in Bedford, MA. This position is onsite with an office setting based in Bedford, MA. Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Formulation Scientist to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, Formulation Lead. Key Responsibilities: Design and execute formulation studies for oligonucleotide drug products Prepare prototype formulations and conduct pre-formulation characterization (e.g., solubility, pH stability, buffer selection) Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Maintain accurate and timely documentation of experiments, protocols, and results in electronic lab notebooks Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional duties as assigned Required Skills &amp; Experience: PhD in Pharmaceutical Sciences, Chemistry, Biochemistry, or related field, Master’s degree with 6+ years of experience in formulation development, or Bachelor’s with 10+ years of experience Experience with nucleic acid-based therapeutics preferred Familiarity with oligonucleotide properties, degradation pathways, and formulation challenges Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I, Formulation

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly experienced and strategic Director/Senior Director of Statistical Programmer to lead and grow our statistical programming function. This role will be instrumental in supporting our expanding clinical development pipeline, including a pivotal phase 3 trial. The ideal candidate will bring deep technical expertise, leadership experience, and collaborative mindset to help drive successful regulatory submissions and clinical trial execution. The Director/Senior Director will have proven leadership ability in a fast paced, environment and interact with all levels of internal staff as well as external stakeholders, including managing external consultants and CROs. This position will report into the Executive Director, Head of Biostatistics. Key Responsibilities: Provide strategic oversight for statistical programming activities across multiple development programs. Serve as the statistical programmer functional lead for the phase 3 pivotal trial, ensure robust data and analysis workflows to support NDA/MAA submission, including integrated summaries (ISE/ISS), working closely with regulatory, biostatistics, and clinical data management teams Oversee and perform quality control (QC) review of datasets, tables, listing and figures (TLFs) generated by CROs and vendors to ensure accuracy, consistency, and regulatory compliance. Ensure adherence to CDISC standards (SDTM, ADaM, Define-XML) and regulatory agency requirements. Guide programming support for exploratory and ad-hoc analyses to generate clinical and scientific insights. Establish and implement processes to ensure quality control, traceability, and reproducibility of analyses. Develop and maintain programming standards, SOPs, and QC procedures that align with regulatory expectations. Manage vendor relationships to ensure deliverables are on time, high-quality, and audit-ready. Partner cross-functionally to support clinical study reports (CSRs), data monitoring committees, and regulatory interactions. Provide leadership in planning for future in-house statistical programming capabilities, including team building and process development as needed. Why Join Us: Opportunity to build the statistical programming function in a growing biotech. Direct involvement in advancing multiple drug candidates, including a pivotal phase 3 program. Small, agile team environment with visibility and impact at the highest levels of the company. Mission-driven organization focused on developing disease-modifying therapies for patients with high unmet medical need. Required Skills &amp; Experience: Master’s degree (or higher) in statistics, Computer Science, Mathematics, or related field. 10+ years statistical programming experience in biotech industry. Proven track record leading programming for pivotal phase 3 trials and regulatory submissions (NDA/MAA/BLA). Hands-on expertise in SAS and R programming, with strong knowledge of CDISC SDTM, ADaM, Define-XML, and FDA submission standards. Experience managing external vendor/CROs in a global clinical development environment. Experience with machine learning programming is a plus. Knowledge of AI/ML is a plus. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

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