Stoke Therapeutics is a biotechnology company that focuses on developing RNA-targeted medicines to treat severe genetic diseases. Founded in 2014, Stoke Therapeutics utilizes its proprietary technology platform to address the underlying causes of a range of genetic disorders. The company's innovative approach has the potential to transform the treatment landscape for these conditions, offering new hope to patients and their families.

Research and Development Specialist

Sr. Scientist, Formulation and GMP Manufacturing

Research Scientist in Neurology

Pharmacology Expert

In Vivo Research Specialist

Senior Scientist, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly skilled and motivated Senior Analytical Scientist to join our Analytical and Formulation Sciences team. The ideal candidate will have strong hands-on experience in UPLC method development, and mass spectrometry-based characterization of oligonucleotide and conjugates. This role is critical to supporting the development of innovative oligonucleotide-based therapeutics. This position will report into the Director, CMC. Key Responsibilities: Develop, optimize, and validate UPLC methods for the analysis of oligonucleotides and their conjugates. Conduct mass spectrometry (LC-MS, HRMS, MS/MS) analyses to characterize oligonucleotide and its conjugates. Interpret analytical data to support product development, stability studies, and release testing. Collaborate with cross-functional teams including Process Development, Quality, and Regulatory Affairs. Author technical reports, method development summaries, and regulatory submissions (e.g., IND, BLA). Perform routine formulation preparation and characterization of oligonucleotide drug products for preclinical studies. Required Skills &amp; Experience: Ph.D. in Analytical Chemistry, Biochemistry, Pharmaceutical Sciences, or a related field with 6+ years of relevant industry experience; or M.S. with 10+ years of experience; or Bachelor’s with 16+ years of experience. Proven experience in UPLC method development for oligonucleotides or nucleic acid-based therapeutics. Strong expertise with mass spectrometry for structural and quantitative analysis of oligonucleotide and conjugates is desired. Strong data analysis skills and proficiency with analytical software (e.g., Empower, MassLynx, Xcalibur). Excellent communication, documentation, and organizational skills. Ability to work independently and collaboratively in a fast-paced environment. Knowledge of ICH guidelines and regulatory expectations for analytical method validation. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is based in Bedford, MA. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Analytical Scientist

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a Director, Engineering &amp; Facilities to manage the ongoing operations and labs within our current headquarters in Bedford. Importantly, this person will also oversee and manage alongside the Chief Financial Officer the ongoing search for a potential new headquarters for Stoke. Therefore, this role is critical not only to develop an efficient, safe, and scalable environment across both office and lab settings, but also an opportunity to play an important role in Stoke’s potential move into our new home. It's a hands-on, strategic opportunity for someone who thrives in a fast-paced environment. This position will report into the Chief Financial Officer. Key Responsibilities: Help manage the process for Stoke’s potential new headquarters search. Project manage construction of new offices, if applicable. Oversee technical aspects of new lab construction, including ensuring sufficient lab space and understanding building requirements. Oversee day-to-day operations of the facilities and the functioning of all building systems. Support the design, construction, startup and operation of all locations. Supervise external real estate, architectural, engineering and contractor relationships. Proactively engage with internal cross-functional teams to achieve site goals and ensure the facility is operating smoothly and in compliance with all health and safety standards. Develop and formalize approval of documents related to site operations including SOPs, safety training, policies and procedures. Ensure building operations comply with all local building codes, zoning laws and regulations. Establish and maintain relationships and contracts with external vendors for services such as ULS inventory management, Airgas, FedEx, and hazardous waste disposal. Review fixed asset inventory and update as required. Maintain and improve site compliance related to environment, health, and safety. Develop and implement processes necessary to operate the facility, including non-GMP cleaning/housekeeping, badging/security, and pest control. Make recommendations to promote efficiencies and apply a proactive approach to potential operational/environmental hazards/risks. Manage projects in support of space planning and expansion across all locations to meet hiring requirements. Develop and maintain budgets and long-range facilities plans based on company growth and future needs. Additional responsibilities as assigned. Required Skills &amp; Experience: Bachelor's degree in engineering or related discipline preferred or equivalent combination of education and work-related experience. Minimum of 10 years overall experience in or related to biotechnology or pharmaceutical industry required. Must have experience in maintenance and construction projects and service vendor management. Significant project management skills in all areas of facilities operations. Ability to read and interpret architectural prints, job specifications etc. Knowledge of OSHA guidelines, and other federal, state and local regulations is required. Strong focus on working collegially with team members from across the organization. Pharmaceutical GMP and GLP knowledge preferred. Experience with asset, maintenance, and facilities management systems. Working knowledge of EH&amp;S. Meet the physical requirements of this role including stooping, standing, walking, climbing stairs/ladders, and the ability to lift/carry up to 50 lbs. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is onsite with an office setting based in Bedford, MA. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Engineering & Facilities

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Director of Regulatory Affairs will be responsible for developing and executing global regulatory strategies for product development and approval in alignment with the corporate strategy for Stoke’s portfolio.&nbsp; Manages compounds through all phases of development, including post approval. Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Develop and implement global regulatory strategies and plans for assigned programs. Develop risk assessment plans. Proactively anticipates risks and establishes risk mitigation with team and management. &nbsp; Ensure project team colleagues, line management and key stakeholders are appraised of developments that may impact regulatory success, exercising sound judgement and communicating in a professional and timely manner. Lead regulatory sub-team and represents RA in product development project teams. Continually expand therapeutic area knowledge, and ensures strategic messaging and content of global regulatory dossiers. Lead cross-functional stakeholders and regulatory professionals to ensure inclusion of appropriate and clear strategic messaging in the content of global regulatory dossiers and responses to regulatory Agency requests. Direct point of contact with health authorities, leads and manages FDA meetings. Prepare and deliver effective presentations for external and internal audiences on a variety of drug development topics. Contribute to the continuous improvement of existing processes and strategies, providing recommendations in their area of expertise. Monitors and anticipates industry trends that impact both the regulatory and access environments to strengthen product development plan(s) and adopt regulatory strategies in a timely manner. Required Skills &amp; Experience: 10+ years minimum of Regulatory Affairs experience in the pharmaceutical industry. Rare disease development preferred. BS., M.S. or Ph.D. in a scientific discipline preferred. Strong working knowledge of drug development processes and regulatory requirements. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Strong interpersonal skills and proven ability to effectively manage a variety of senior personnel including medical, scientific, and manufacturing staff. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Regulatory Affairs, Global Regulatory Strategy

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Director/Senior Director, Medical Affairs (Midwest) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary. Location(s): Stoke operates in Bedford, MA.&nbsp; This position is remote, field- based in the continental United States, and is responsible for a regionally designated area. Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Medical Affairs - Midwest

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Reporting into the SVP of Global Medical Affairs, the Executive Director is responsible for providing leadership and strategic direction to a team of US based Regional Medical Directors (RMD) to establish collaborative and scientific engagement with clinical experts to help advance our medicines for patients. Key Responsibilities: Leads and oversees ongoing development of a team of rare disease US Regional Medical Director team that helps advance engagement with Stoke and external credibility of our science. Responsible for overall day-to-day support of all operational and leadership aspects of the RMD team including onboarding, goal setting, coaching and ongoing development of team and individual talent. Work closely with clinical operations and development teams to build and foster collaborative research relationships with academic and community KOLs in support of Stoke clinical studies. Develop and maintain credible, scientific relationships with academic and medical thought leaders. Works closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, staffing medical booths, gathering competitive intelligence, and developing insight reports. Develop, share, and discuss ongoing clinical insights with program team and cross-functional members to ensure awareness and understanding of key opinion leader perspectives to inform and guide strategic decision-making. Work closely with the RMDs to develop and implement strategic territory plans, including KOL engagement plans for top centers of comprehensive care within a defined region. Develop, tracks and represents key impact measures and insights to internal partners to ensure ongoing understanding of strategic alignment and impact of medical affairs activities. Collaborate closely with Sr Director of KoL Engagement and other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Ensure appropriate policies and monitoring are in place to ensure appropriate and compliant team activities. Ability to travel 50%+, including the ability to travel overnight, internationally, and occasionally on weekends. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience: Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Excellent verbal and written communication skills, and exceptional interpersonal skills Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills Valid driver's license and the ability to travel as necessary. Location(s): Stoke is located in Bedford, MA.&nbsp; This position may be a remote or hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 50%+ travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director, Field Medical Affairs

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Key Responsibilities: Executive Director/VP, Commercial Operations works cross-functionally to identify and implement best practices, methodologies and processes to achieve commercial and operational excellence. This role will be responsible for the operational build, development and maintenance of commercial systems, forecasting, market research, field data support, policies and governance documents to support both the launch and commercialization of Stoke’s products. This will include planning for weekly performance reports and insights at launch. The role will also be responsible for field force incentive compensation, working closely with field leadership in Sales and Market Access. Additionally, in partnership with IT, they will identify, develop and deploy enterprise functions and platform technology solutions to maximize operational effectiveness, and work closely with third party vendors such as CRM providers and data aggregators. Work closely within Commercial and Finance to build, analyze and communicate Commercial program budgets to ensure accurate forecasting reporting. Responsible for developing and reporting daily/weekly revenue process with Finance at launch. Collaborate with Finance and Manufacturing to develop and support the monthly/quarterly revenue forecasting process. Collaborate with Commercial stakeholders to coordinate and synergize all market research needs and efforts. Work with cross-functional team members to identify and prioritize actionable, high-impact insights across a variety of core business areas including Sales, Medical Affairs, Marketing, Finance, Market Access, Patient Services and IT. Research, design, implement, and validate cutting-edge dashboards to analyze diverse sources of data to achieve targeted outcomes. Institute processes for data and reporting governance of ad-hoc field-based inquiries from the Executive Leadership Team and other Commercial functions. Design, develop, implement and lead the company's data warehouse with input from Commercial, IT, Quality and Finance. Strategic and tactical planning for field teams including management of vehicle allowance, incentive compensation design and administration, data management, reporting, and CRM management. Lead data inquiry and data stewardship efforts in support of field-based teams Collaborate with IT to develop Stoke’s enterprise data strategy Any and all other responsibilities as requested Required Skills &amp; Experience: A minimum of 10 years of pharmaceutical/biotech industry experience. Bachelor’s degree or equivalent experience in Commercial Operations, Business Intelligence, Data Management or Analytics. Good verbal and written communication with solid organizational, time management, and project management skills. Strong personal leadership skills to drive performance and support corporate objectives. Ability to work collaboratively and effectively with others. Ability to work under pressure and meet deadlines. Strong analytical and communication skills. Well organized and detail oriented. Creativity and resilience in problem solving. Relentless hands-on drive to implement and accomplish performance goals. Effective management of vendor partners to ensure project scope is achieved within defined budget. Ability to independently research, design, and implement platform technology solutions. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director/Vice President, Commercial Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Sr. Director, Medical Information will oversee the development and execution of the medical information strategy, ensuring the provision of accurate, balanced, and timely scientific and clinical information to healthcare professionals, patients, and internal stakeholders. This role will collaborate closely with Clinical Development, Medical Affairs, Advocacy, Regulatory, Pharmacovigilance, and other cross-functional teams to support product knowledge and ensure compliance with industry standards. This position reports into the Executive Director, Global Medical Communications. Key Responsibilities: Lead the Medical Information function, ensuring the development of high-quality medical information responses and resources. Oversee the handling of medical inquiries from healthcare professionals, caregivers, patients, and internal teams, ensuring responses align with company policies and regulations. Develop and maintain medical information databases, standard response documents, and FAQs. Ensure compliance with industry regulations, including FDA, EMA, and other global health authorities. Collaborate with cross-functional teams to provide medical insights that inform product development, medical strategy, and commercial activities. Train internal stakeholders, including Medical Science Liaisons and Sales teams, on medical information policies and best practices. Monitor emerging scientific data and competitor activity to keep medical information materials up-to-date. Develop and implement key performance indicators (KPIs) to assess the effectiveness of medical information services. Represent the company in external medical information forums and industry meetings. Required Skills &amp; Experience: Advanced degree (PharmD, MD, PhD, or equivalent) in a life sciences field. Minimum of 7-10 years of experience in Medical Information or related roles within the pharmaceutical or biotechnology industry. Strong knowledge of global regulatory guidelines governing medical information activities. Proven leadership experience with the ability to develop and manage high-performing teams. Excellent communication and presentation skills, with the ability to translate complex scientific data into understandable content for diverse audiences. Proficiency in medical literature evaluation and database management. Ability to work cross-functionally in a fast-paced, innovative environment. Experience with medical information systems and technology platforms is preferred. Location(s): Stoke operates sites in Bedford, MA.&nbsp; This position is hybrid/remote based position that will require a consistent presence with our office based staff. Travel: This position will require approximately ~10% travel, including occasional presence at internal meetings or conferences as required.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Global Medical Information

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Director, Market Access Strategy &amp; Marketing will lead the development and execution of strategies and materials in support of a potential launch of the first ever disease modifying therapy in Dravet Syndrome, along with future medicines in our pipeline. This includes assessing market dynamics and policies, developing strategies and tactics that communicate the value of our therapy(ies) to key market access stakeholders. You will play a pivotal role in ensuring payer readiness, supporting product reimbursement, and aligning internal teams on access strategy during pre-launch and launch phases. This role will also support strategy and marketing for other functions within the market access department such as Distribution, Field Reimbursement, and Nurse Education. This role is ideal for a resourceful, cross-functional leader who thrives in a start-up environment. Key Responsibilities: Strategy Development Lead the development of the U.S. market access marketing strategy aligned with the broader commercial and brand strategy. Define and communicate the product’s value proposition for payer and organized customer audiences. Contribute to the development of pricing and reimbursement strategy in partnership with Market Access leadership. Tools &amp; Resources Translate scientific and clinical data into compelling, payer-relevant value communications tailored to rare disease decision-makers. Lead development of payer marketing materials, including AMCP dossiers, payer decks, value proposition tools, and digital content. Develop and manage the creation of core market access marketing materials including unbranded and branded payer decks, early engagement tools, and payer advisory content. Plan and oversee future development of AMCP dossiers, payer engagement models, and field access tools. Support early access initiatives and pre-approval information exchange strategies. Develop field market access materials and training to support Account Directors and other market access Field Teams. Manage agency partners effectively and stay on time and within budget Customer Engagement Lead insight generation with US payers to inform access pull-through strategy. Support development and implementation of innovative access programs to optimize coverage, reimbursement, and patient access. Cross-Functional Leadership Work closely with Medical Affairs, Brand Marketing, Legal, and Compliance to ensure alignment and approval of materials. Support launch readiness and post-launch execution. Analytics &amp; Insights Leverage market research, payer insights, and competitive intelligence to refine access strategies and messaging. Monitor access environment trends and assess their implications on brand strategy. Required Skills &amp; Experience:&nbsp; Bachelor’s degree required; advanced degree (MBA, PharmD, MPH, etc.) preferred. 10+ years of experience in biotech/pharmaceutical market access, including at least 5 years in payer/market access marketing. Prior launch experience in specialty therapeutics; rare disease and medical benefit preferred. Deep understanding of U.S. healthcare landscape, payer decision-making, and pricing &amp; reimbursement dynamics. Experience developing market access tools and engaging with internal and external stakeholders. Strong strategic thinking, communication, and project management skills. Comfortable working in a fast-paced, entrepreneurial, and highly collaborative environment. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Market Access Strategy & Marketing

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Director, Regulatory Labeling, Advertising &amp; Promotion will serve as an expert on FDA regulations, guidance, and enforcement trends governing the labeling development and promotion of Stoke’s products. Provides regulatory oversight to ensure regulatory compliance of promotional materials generated for assigned investigational medicinal products and subsequently approved products. Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Responsible for strategy, development, and implementation of labeling as well as systems to manage labeling lifecycle. Provide label strategies to ensure claims can be supported by development. Lead cross-functional Labeling teams to align labeling strategy and content. Develop and maintain the target product profile, the company core datasheet, the patient labeling, the USPI, and the summary of product characteristics or international equivalents. Provide regulatory advice and information to cross-functional teams and to other functional areas (i.e., CMC, nonclinical, and clinical) for product development and planning to support the timely achievement of company and department goals. Interpret regulations and guidance relevant to the development and implementation of labeling. Provide strategic oversight to ensure promotional materials and non-promotional materials generated for external communication regarding commercialized products and/or compounds in development adhere to regulatory compliance standards. Develop systems for material content review and approval. Provide regulatory input and guidance to relevant stakeholders regarding marketing strategies, promotion objectives, and plans. Represent regulatory at promotional review meetings to assist commercial with development of compliant promotional materials. Primary liaison with FDA’s OPDP. Collaborate with Regulatory Affairs, Medical, Legal, Commercial, Ad Agencies, and Compliance to ensure alignment and effective execution of corporate objectives while mitigating potential regulatory risk. Required Skills &amp; Experience: A BS/BA degree or equivalent, and a minimum of 12 years’ experience in Regulatory Affairs specific to Regulatory Affairs Labeling, Advertising and Promotion. Knowledge of applicable regulations and regulatory guidance specific to labeling, advertising and promotion of pharmaceutical/biologic products for healthcare professionals, payor and consumer audiences. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Proven ability to manage multiple complex projects, with the flexibility and adaptability to re-prioritize workload. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Regulatory Labeling Development and Advertising & Promotional

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Sr. Manager, GCLP Compliance, is responsible for overseeing all aspects of compliance for GCLP testing for Stoke’s clinical development programs with Stoke’s Contract Research Organizations (CROs) partners. This person will be a key contributor in growing and shaping Stoke’s GCLP quality monitoring program and will be responsible for supporting Stoke’s DMPK study team towards ensuring data quality and compliance with regulations (e.g., FDA, EMA, etc.). This candidate will be required to contribute to the establishment and maintenance of Stoke’s Quality Management System for the areas of GCP and GCLP. This person will partner with Stoke’s Quality, DMPK and clinical study teams to create a quality culture within Stoke and help reach a sustained state of inspection readiness. This role requires the person to identify and effectively communicate compliance risks and drive action plans to mitigate risks. This role will report into the Director of Clinical Quality and will interface closely with other cross-functional groups such as DMPK, Clinical Development, Clinical Operations, Regulatory and other functions with our vendors and across the Stoke organization. The ideal candidate will have a strong working knowledge of relevant GCP/GCLP FDA, EU, ICH guidelines, and regulations and experience working with CROs and vendors. This candidate should have experience in auditing and a demonstrated ability to communicate significant observations in a sound and factual manner. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for quality management is essential to this role. The candidate should be willing to roll up their sleeves and directly contribute to the implementation of processes and resolution of issues as required. Key Responsibilities: Contribute to the development of procedures to oversee the quality component of Stoke’s GCLP/ Bioanalytical work at the vendors. Provide support with vendor qualification and management. Partner closely with members of the DMPK and Clinical Development Operations teams to support the successful and compliant execution of GCLP bioanalytical work to meet regulatory requirements, guidelines, internal policies, and procedures. Collaborate with Subject Matter Expert’s (SMEs) to develop, maintain and report on metrics from quality monitoring plans. Support internal, in-process, study, and process audits related to GCLP activities and bioanalytical studies. Oversee and assist with audits of vendors for GCLP Bioanalytical laboratories, as necessary, and work with Stoke’ technical SMEs staff to ensure the implementation of appropriate CAPAs as applicable. Support investigations into GCLP non-compliance. Partner with CROs and the DMPK and clinical study team to analyze investigation findings to identify root/probable cause. Ensure adequate investigation, documentation, and implementation of appropriate CAPAs. Assure timely reporting of potential or confirmed violations, as appropriate, to regulatory agencies. Represent QA on project teams, as needed and provide GCLP compliance interpretation, consultation, training, and other supportive services necessary to maintain and improve the quality of research for timely regulatory submissions. Help train/prepare Stoke employees for regulatory inspections. Provide input to and review of responses to regulatory agency questions resulting from regulatory inspections. Contribute to the development of Stoke’s Quality culture. Required Skills &amp; Experience: S. in Biochemistry, Chemistry, Biology, Pharmaceuticals&nbsp;or equivalent with a minimum of 10+ years’ combined experience in a GCP/GLP environment supporting the development of biopharmaceutical products. Experience in RNA based therapeutic modalities preferred. Fully versed in GCP/GLP, ICH Guidelines, FDA, and European regulatory agency requirements (understanding of other regulatory guidelines for other countries a plus). Experience building systems from scratch and scaling processes. Practical knowledge of GCLP related to audits and business standards. Attention to detail with the ability to work within a multi-disciplinary team, as well as with external partners and vendors to establish and maintain a strong quality relationship. Ability to make decisions that are guided by applicable regulatory mandates, Stoke and designated vendor policies, procedures, and business plans. Ability to work autonomously, provide QA advice, effectively manage time, and deliver results on time. Ability to collaborate effectively in a dynamic, cross-functional matrix environment. Strong verbal and written communication skills. Conflict resolution/management and negotiation skills. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, GCLP Compliance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking an enthusiastic and experienced bioinformatician to join the computational group focusing on mRNA splicing and antisense oligonucleotides. Drug discovery at Stoke begins with computational biology. As part of the early discovery team led by Stoke’s co-founder, you will work collaboratively with data and experimental scientists to expand our analytical approaches in identifying therapeutic targets relevant to the TANGO technology. Key Responsibilities: Assist in the development of strategic plans for analyzing and deciphering next-generation sequencing data, and apply modern computational biology approaches to advance Stoke's portfolio of therapeutic targets. Propose and develop analytical and visualization tools to deliver complex biological data. Build, deploy, and maintain bioinformatics applications on Amazon Web Services. Contribute to the development and maintenance of web applications to facilitate target identification and data analysis. Use computational tools to investigate novel approaches to upregulate gene expression. Provide program-specific support for pre-clinical and clinical groups, such as biomarker development and mechanism of action of ASO clinical candidates. Support a highly collaborative environment and promote positive teamwork among a team of scientists from different backgrounds. Communicate scientifically rigorous results clearly, verbally and in writing, both internally and externally. Required Skills &amp; Experience: A degree in bioinformatics, computational biology, computer science, mathematics, systems biology, or any related quantitative field. Previous experience in the biotech or pharma industry; 14 years with a bachelor’s degree, 8 years with a master’s degree, or 4 years with a Ph.D. &nbsp; Experience in and deep understanding of posttranscriptional gene expression regulation, including but not limited to RNA splicing, RNA surveillance. pathways, RNA binding proteins, and antisense oligonucleotides. Fluency in coding languages such as Python and R, including scientific modules and packages commonly used in bioinformatics. Familiarity with machine learning algorithms and applications is a plus. Direct experience in developing and maintaining reproducible analytical workflows for high-throughput sequencing assays such as bulk or single-cell RNA-Seq, eCLIP, or whole-genome sequencing using workflow management systems such as Snakemake or Nextflow. Management experience in leading computational scientists and interns. Location(s):&nbsp;&nbsp; This position is a hybrid position with an office setting based in our Bedford, MA location.&nbsp;&nbsp; Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Scientist, Bioinformatics

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking a highly motivated and experienced Executive Assistant to join a company pioneering genetic disease treatments. This is a pivotal executive support role for someone who brings composure, precision, and a genuine commitment to enabling leadership excellence at the highest level. The successful candidate will provide direct support to the Chief of Corporate Affairs and Strategic Partnerships, Chief Medical Officer, Chief Regulatory Officer, and their teams Key Responsibilities: Provide general administrative support to the Chief Medical Officer, Chief Regulatory Officer, and Chief of Corporate Affairs &amp; Strategic Partnerships, as well as their teams. Manage calendars, schedule meetings/events, and handle all other administrative activities for assigned executives and their associated teams. Coordinate activities/meetings as needed interdepartmentally. Plan and support complex meetings with internal stakeholders and Key Opinion Leaders, investors, and other external parties. Prepare and organize meeting materials, including agendas, presentations, and follow-up documentation for internal and external stakeholders Arrange or set-up AV for hybrid, remote, or in-person meetings and trouble-shoot basic AV technology issues for conference calls and interface with IT and conference services for advanced matters. Process and reconcile expense reports promptly and accurately. Serve as a liaison and travel coordinator for departmental visitors. Create, manage and track purchase orders as requested. Provide cross-coverage and proactive collaboration within the administrative team. Assist the administrative team on company-wide projects as requested. Handle additional special projects as assigned.&nbsp;&nbsp;&nbsp; Required Skills &amp; Experience: Bachelor’s degree required with a minimum of 5+ years relevant experience. Experience working in a Biotech environment preferred. Proficiency in MS Office with expertise in Microsoft Word, PowerPoint and Excel. Detail oriented, flexible and comfortable working in a fast-paced office environment. Exceptional verbal and written communication skills. Superior organizational skills and dedication to completing projects with quality and in a timely manner. Must be a solution-oriented team player. Must possess forward thinking skills, creativity and critical thinking skills. Ability to maintain high level discretion and confidentiality. Location(s): This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $109,000 - $132,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Assistant

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: We are seeking a dedicated and highly motivated Principal Scientist in Oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists in managing the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines. Successful candidates will have significant experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report to the Director, Chemistry. Key Responsibilities: Independently design and implement scientific and synthesis strategies relating to the research and development of antisense and other synthetic oligonucleotide therapeutics. Contribute to multiple projects and provide key technical knowledge to the project team. Plan and execute solid-phase oligonucleotide synthesis for SAR campaigns on several projects. Participate in the identification, development, validation and implementation of both current and next-generation technologies to enable proprietary ASO therapeutic development including purchase and installation of hardware and software supporting synthesis, purification, and characterization of oligonucleotides. Demonstrate working knowledge of conjugation chemistry and techniques aimed at producing novel conjugates. Demonstrates understanding of the chemistry of nucleosides, nucleotides, and oligonucleotides as applied to antisense and other oligonucleotide technologies. Stay current with scientific literature and understanding of the state of the art in oligonucleotide chemistry, purification, and screening strategies. Work with both internal chemistry team and external CRO’s to generate innovative solutions to synthetic and drug design challenges within the context of oligonucleotide drug discovery projects. Authors reports and manuscripts. May contribute to patent filings and/or regulatory filing. &nbsp; Required Skills &amp; Experience: PhD in Oligonucleotide Chemistry and relevant experience in a drug discovery environment with at least 8 years of oligonucleotide synthesis experience in a drug discovery environment, Master’s degree with 12+ years of experience or Bachelor’s degree with 18+ years of experience. Strong practical knowledge of design, synthesis, and purification of synthetic oligonucleotides. Experience in scale-up synthesis would be a plus. Excellent project/time management, and problem-solving skills. Excellent written and verbal communication skills, including making effective and compelling presentations in both large and small groups and communicating complex scientific and nonscientific issues to program teams. Strong people skills and the ability to thrive in a team and goal driven environment Experience designing, synthesizing and purifying oligonucleotides to support research and scale up studies. Proven track record of scientific contributions in oligonucleotide and conjugates research as evidenced by publications/presentations/patent&nbsp;applications and/or the identification of preclinical development candidates. Location(s): Stoke operates in Bedford, MA. This is a lab-based position based in our Bedford, MA location. Travel: This position will require approximately 0% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $169,000 - $191,000 The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Principal Scientist, Oligonucleotide Chemistry

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose:&nbsp; We are seeking experienced Associate Scientist who will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of CNS diseases. This position will report to a Principal Scientist in the in vitro pharmacology group. As part of the group you will work collaboratively to design and perform gene and protein expression assays to support development of new ASO-based therapies. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types. Experience with iPSC-derived CNS cell types is a plus. Evaluate oligonucleotide-based drug candidates in cell-based assays. Conduct in vitro disease model cellular assays to evaluate drug candidates. Design, execute and troubleshoot routine experiments independently. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, qPCR, western blotting and other project-specific assays, as necessary. Familiarity with ELISA and capillary-based protein assays in a plus. Use microscopy and immunohistochemistry-based methods to evaluate cellular phenotypes. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts. Present research findings at group meetings. Work independently as well as collaborate with peers in a result-oriented research environment. Required Skills &amp; Experience:&nbsp; BS with a minimum of 7 years post-graduate experience or M.S. with a minimum of 4 years of experience in neuroscience, biotechnology, cell and molecular biology or related field, preferably in the biotech or pharma industry. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong Hands-on experience in RNA isolation techniques, mammalian cell culture and cell-based assays is strongly desirable. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is an on-site position located in Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, in vitro Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Stoke is seeking an enthusiastic and highly motivated Senior Research Associate to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. Key Responsibilities: Design and execute routine studies to investigate the PK/PD and efficacy of drug candidates. Perform routine to intermediate handling procedures and injections, monitor animal health, and collect tissues in mice. Perform moderately complex behavioral experiments in animal models. Support the implementation of new animal models. Demonstrate technical proficiency to investigate, identify, develop, optimize, and troubleshoot new methods/ techniques/ instrumentation to address critical project needs. Read and understand scientific literature. Analyze data, author technical reports, and document experiments in an electronic notebook. Participate and present at project team and departmental meetings. Maintain laboratory organization including equipment upkeep and ensure compliance with safety protocols. Collaborate across functional areas. Required Skills &amp; Experience: BS with 7+ years post-graduate experience or M.S. with 4+ years of experience working in a biotechnology or pharmaceutical company or contract research organization. Experience working with animal disease models. Experience with animal behavioral assays. Experience with subcutaneous and intravenous injections in rodents; intracerebroventricular injection experience a plus. Experience with molecular biology assays such as PCR, ELISA, MSD, BCA, Western Blot (or JESS). Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Experience in neuroscience a plus. Experience with ISH/IHC a plus. Location(s): This position is based in our Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: we are seeking a dedicated and highly motivated senior research associate in oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists on the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines while reporting to your manager. Successful candidates will have experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report into the Director, Chemistry. Key Responsibilities: The role is primarily responsible for supporting the automated synthesis, cleavage, deprotection, purification, analysis and packaging of oligonucleotides, including heavily modified oligonucleotides. Maintaining laboratory equipment, including the oligonucleotide synthesizer, HPLC machines and benchtop equipment to ensure consistent and reliable oligonucleotide production. Performing critical evaluation and optimization of the processes to improve synthesis yield, purity and timelines for production of modified oligonucleotides Manage ordering and organization of chemicals and supplies necessary for oligonucleotide synthesis Analyzing and interpreting data as well as writing technical reports and protocols, with supervision. Presents research results at lab meetings. Keeps up to date on relevant scientific literature and developments to support ongoing research efforts. Required Skills &amp; Experience: S. with 4 years of experience or M.S. with 2 years of relevant industry experience Hands-on experience with Oligo synthesis (mid and large scale preferred). Experience with MerMade 12 and 192 synthesizers preferred. Experience in preparing buffers for purification and maintaining lab instruments such as AKTA purifiers, UF, HPLC and LC-MS. Previous experience using Microsoft Excel, Word, PowerPoint and Chemdraw Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Research Associate, Oligonucleotide Chemistry

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: Stoke Therapeutics is seeking to hire to an Associate Scientist who will support ongoing research efforts in designing and evaluating antisense therapies for diverse targets focusing on CNS and/or Cardiovascular rare diseases. This position will report directly to a Principal Scientist in the in vitro pharmacology group. The ideal candidate will provide hands on laboratory experience to design, implement and execute research assignments for a specific project/projects. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types and cell lines. Experience with culture and differentiation of human iPSC-derived neurons and cardiomyocytes. Perform gene perturbation and pharmacological studies. Evaluate oligonucleotide-based drug candidates in cell-based assays. Conduct in vitro disease model cellular assays to evaluate drug candidates. Design, execute and troubleshoot routine experiments independently. Perform cell-based and molecular assays such as – RNA extraction, qPCR, RT-PCR, protein extraction and specific assays as necessary. Experience and familiarity with ELISA, MSD assay, AlphaLISA assay, western blot and capillary-based protein assays (JESS). Familiarity with plate-based assay development and Nano String is a plus. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts Present research findings at group meetings. Work independently as well as collaborate with peers in a result-oriented research environment. Additional work as assigned Key Requirements:&nbsp; B.S with 7 years post-graduate experience or M.S. with 4 years of experience preferably in Biology, biotech or pharma industry. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong Hands-on experience in RNA isolation techniques, mammalian cell culture and cell-based assays is strongly desirable. Strong hands-on experience in plate-based assays. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): Stoke operates in Bedford, MA. This position is located in Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Associate Director/Director, Clinical Science will serve as a scientific and analytical leader, working cross-functionally to define, plan, and execute key clinical data analyses to support clinical development and publication strategy, regulatory submissions and other integrated activities. The successful candidate will bring a strong foundation in clinical science and data analysis, with a demonstrated ability to translate scientific questions into visual figures and tables. The Associate Director/Director of Clinical Science will be a key liaison between clinical development, biostatistics, data management, medical affairs/publications, communications and regulatory affairs, driving analytical deliverables across multiple studies and programs. This role also includes broader clinical scientist responsibilities including leading strategic clinical development initiatives across multiple indications. This individual will provide key input into clinical development activities that include trial design and development of clinical study documents and communications, focusing on data collection and data interpretation. This position will report into the Senior Director, Clinical Science. Key Responsibilities: Clinical Data Analysis Strategy (Primary Focus): Lead cross-functional efforts to identify clinical data analysis needs across studies and programs Define and drive the clinical data analysis strategy to support clinical decision-making, safety/efficacy evaluation, publications and communications, and regulatory filings Collaborate with biostatistics, and data management to access, structure, and interpret clinical trial datasets and TLFs Perform and/or support exploratory and ad hoc analyses to support internal discussions, publications/communications and regulatory responses Ensure analytical outputs align with scientific objectives and regulatory expectations, maintaining data integrity and consistency across documents Present complex data findings clearly to cross-functional teams and externally as needed Clinical Science Responsibilities: Develop, co-author and review key strategic documents including but not limited to Clinical Development Plans, Protocol Concepts and Full Protocols, Investigator Brochures, Briefing Documents, Safety Updates, IND/NDA submission documents, Responses to Health Authorities Inquires and Clinical Study Reports in conjunction with other members of the clinical study/program teams as needed Serve as an interface for clinical studies between clinical development and multiple internal functions including clinical development operations, data management, biostatistics, regulatory, program management and DMPK/biomarkers and external teams as needed for clinical-development initiated projects Develop expertise in core clinical assessments incorporated in clinical studies and provide education and training to key stakeholders, including at investigator meetings, as needed, in order to ensure quality completion Support medical monitoring activities, including review of eligibility, safety data review and trend identification Collaborate with functions including data management to continuously enhance processes for the timely and efficient internal review of clinical data Develop effective working relationships with key stakeholders including investigators to optimize scientific quality and innovation in clinical study design, execution, reporting and publication Represent clinical development at advisory boards, investigator meetings, site initiations, and other external engagements Attend scientific meetings to interact with investigators and advisors and to present clinical data, as needed Required Skills &amp; Experience: Advanced degree required (e.g., M.S., PharmD, or Ph.D. ) in a life science, clinical research or another similar field At least 7 years of clinical drug development experience Experience in neurology indications preferred and rare disease indications highly preferred Strong knowledge of FDA/EMA/ICH requirements and good clinical practice Strong analytical ability including familiarity with TLFs/statistical outputs and proficiency in data analysis tools (e.g., Prism, RStudio, JMP) Experience having close collaboration with data management, biostatistics/programming teams Exceptional written and oral communication skills tailored to varied audiences Proven ability to work independently and collaboratively, as required, in a dynamic, fast-paced start-up environment consisting of internal and external team members Strong interpersonal skills commensurate with the need to work closely with a broad range of stakeholders, from investigators to internal teams Attention to detail and a commitment to delivering high-quality work Strong planning, organization and time management skills including the ability to prioritize multiple, complex initiatives A sense of passion and urgency to achieve goals and improve the lives of patients Location(s): Stoke operates in Bedford, MA. This position is hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director/Director, Clinical Science- Data Strategy and Analysis

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Research Associate will support ongoing research efforts in designing and evaluating antisense therapies for diverse group of diseases. This position will report to a Senior scientist in the Discovery Biology Group. Key Responsibilities: Work in aseptic conditions and maintain cultures of various cell types. Screen and evaluate ASOs in relevant mouse tissues and cell-based assays. Perform cell and molecular biology techniques such as RNA, DNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Troubleshoot experiments, analyze and interpret data, participate in development of new assays with support from more senior scientists. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Present research findings at group meetings. Required Skills &amp; Experience:&nbsp; S with 2 years of post-graduate experience in molecular and cell biology research, preferably in industry or a Master’s degree in molecular or cellular biology. Strong background in cell and molecular biology research; understanding of the basics of PCR is a must. Strong hands-on experience in RNA isolation techniques from cells and tissues, mammalian cell culture and cell-based assays is strongly desirable. Strong organizational and recordkeeping skills is a must.&nbsp; Ability to adapt to changing priorities and deadlines. Excellent planning and time management skills including the ability to support and prioritize multiple projects. Location(s): This position is onsite in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Research Associate, Biology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Vice President, Commercial Manufacturing – Rare Diseases is responsible for the strategic and operational leadership of all commercial manufacturing activities supporting the company’s rare disease portfolio. This executive will ensure reliable, compliant, and cost-effective production of highly specialized oligonucleotide therapies.&nbsp; The VP will lead external manufacturing networks to ensure product availability for patients with critical unmet medical need. This position will report into the SVP, Chemistry and Manufacturing. Key Responsibilities: Strategic &amp; Operational Leadership Develop and execute a comprehensive commercial manufacturing strategy aligned with the company’s mission to deliver therapies for rare and ultra-rare diseases, maintaining appropriate levels of inventory to meet/exceed demand. Oversee technology transfer from late-stage development into commercial GMP production, ensuring scalability and robustness for niche patient populations. Interface with internal SMEs to support manufacturing operations and potential investigations or troubleshooting of ongoing manufacturing activities.&nbsp;&nbsp; Quality, Compliance &amp; Regulatory Ensure all manufacturing activities meet or exceed global regulatory standards (FDA, EMA, PMDA, MHRA, etc.). Partner with Quality and Regulatory Affairs to maintain GMP compliance, validation, and audit readiness. Embed a culture of safety, quality, and continuous improvement throughout all operations. Supply &amp; Lifecycle Management Direct supply planning and capacity management to ensure uninterrupted global supply for commercial and compassionate-use programs. Support product lifecycle management, including post-approval process optimization and change control. Manage risk mitigation and business continuity strategies for specialized therapies and limited-source materials. Financial &amp; Business Leadership Develop and manage operating budgets, capital investment plans, and cost optimization programs without compromising quality or compliance. Support strategic business decisions, as well as corporate partnerships.&nbsp; Required Skills &amp; Experience: Advanced degree in Engineering, Biochemistry, Chemistry, Pharmaceutical Sciences, or related field; MBA and/or PhD preferred. 15+ years of experience in pharmaceutical or biopharmaceutical manufacturing, with at least 8 years in senior operational leadership roles. Recent&nbsp; NDA experience, including authorship of module 3 for submission. Recent commercialization experience.&nbsp; Demonstrated experience with rare disease products (oligonucleotide modalities would be a plus). Deep understanding of GMP regulations and quality systems supporting GMP commercial manufacturing Strong leadership, strategic planning, and cross-functional collaboration skills. Exceptional communication and executive presence, with a track record of influencing internal and external stakeholders.&nbsp; &nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office based in Bedford, MA location. Travel: This position will require approximately 30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $320,000 - $355,000 The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Commercial Manufacturing – Rare Diseases

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Senior Research Associate to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, formulation lead. Key Responsibilities: Support the design and execution of formulation studies for oligonucleotide drug products Perform laboratory experiments in a safe and efficient manner and record research findings in ELN Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional tasks as assigned Required Skills &amp; Experience: B.S. with 4 years of experience or M.S. with 2 years of experience in Chemistry or Organic Chemistry Prior industry experience&nbsp;in oligonucleotide formulation and scale-up is desired Sound knowledge of analytical chemistry and basic nucleic acid chemistry is preferred Experience with UPLC, LCMS and formulation characterization instrument is desired Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Research Associate, Formulation

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: &nbsp; The Senior Manager, CMC Project Management will have responsibility for delivering operational success across the entire development portfolio. This role will support the integrated project management for drug substance, formulation development, analytical development (drug substance and drug product) and packaging &amp; labeling. The candidate will be accountable for team deliverables, ensuring operational success with respect to timelines, and attainment of technical, regulatory and goals/milestones. This person will also be leveraged as a thought partner for the CMC function that works closely with QA and Regulatory CMC to ensure all key stakeholders are aligned with the overall CMC strategy to support the programs. This position will report to the Director, CMC Project Management. Key Responsibilities: Responsible for cross-functional project management across CMC subfunctions (drug substance, drug product, analytical, label &amp; package) and their outsourced manufacturing partners for early phase clinical programs. Plan, manage, and track integrated CMC activities to ensure uninterrupted supply of clinical trial materials; escalate risks as appropriate. Support RFP generation and contract management with outsourced manufacturing partners. Support budget preparation and tracking. Liaison between CMC and other departments (e.g., Quality, Clinical, Regulatory) for early phase clinical programs. Support and/or represent CMC function at program level meetings. Provide project management support as needed for late phase and commercial programs. Coordinate with alliance management personnel for partnered programs as needed. Demonstrate best practices for meeting management and knowledge management. Other duties as assigned. Required Skills &amp; Experience:&nbsp; Bachelor’s/Master’s degree in a discipline relevant to project management, pharmaceutical research, development or manufacturing with relevant industry experience. Professional certification in project management (e.g. CAPM, PMP, PRINCE2, or equivalent) preferred. Minimum of 2 years of experience required in a GMP drug substance or drug product development and manufacturing environment for small molecules, oligonucleotides, polypeptides, or biologics. Direct experience working at CDMOs or in a role with strong CDMO oversight. Experience with Quality requirements for batch release and disposition, as well as general Quality Assurance procedures. Proven success working in cross-functional teams including QA and regulatory CMC. Strong scientific background and knowledge of the drug development process. Experience with Microsoft Project/Smartsheet, Power Point, Excel, Word along with other related software. Experience with preparation of integrated Gantt charts using OnePager, Office Timeline, or equivalent preferred. Experience in managing multiple programs at one time, can work well with different management styles to keep a project moving. Competency in developing, implementing, and delivering CMC project plans (milestones, timelines, resources, etc.) to successful endpoints. Strong conflict management and negotiation skills. Superb written and oral communication skills. Experience with oligonucleotides is a plus. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 10% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, CMC Project Management

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Scientist I will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of diseases. This position will report to a Principal Scientist in the in vitro Group. Key Responsibilities: Primarily responsible for designing, executing, implementing, and troubleshooting routine studies in collaboration with cross functional groups. Independently design and execute molecular assays to identify preclinical drug candidates targeting disease genes. Develop high-quality cell-based assays to inform disease target gene engagement. Devise and test new strategies and technologies to modify gene expression in collaboration with colleagues. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Work with CRO laboratories and external collaborators to support internal studies. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts and patents with assistance. Present research findings at group, cross-functional team and lab meetings. Acts as a technical resource within own group/project team and collaborates with other interdisciplinary functions. Required Skills &amp; Experience:&nbsp; BS with 10 years post-graduate experience, M.S. with 6 years of experience, or a Ph.D. in cell or molecular biology or a related field. Experience in molecular biology, RNA techniques, and cell-based assays are a must. Previous work experience in anti-sense oligonucleotide-based target validation. Experience in cell culture techniques with variety of cells (previous work experience in the culture and differentiation of human iPSC to neuronal cell types would be beneficial). Work experience in therapeutic areas involving CNS or ocular disease highly desirable. Strong organizational and recordkeeping skills, experience using ELN is highly desirable. Strong oral and written communication skills. Location(s): This position is lab based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I, in vitro Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Scientist I will support ongoing research efforts in designing and evaluating antisense therapies for a diverse group of diseases. This position will report to a Principal Scientist in the Discovery Biology Group. Key Responsibilities: Primarily responsible for designing, executing, implementing, and troubleshooting routine studies in collaboration with cross functional groups. Independently design and execute molecular assays to identify preclinical drug candidates targeting disease genes. Develop high-quality cell-based assays to inform disease target gene engagement. Devise and test new strategies and technologies to modify gene expression in collaboration with colleagues. Perform cell and molecular biology techniques such as RNA and protein isolation, RT-PCR, RT-qPCR, western blotting and other project-specific assays, as necessary. Work with CRO laboratories and external collaborators to support internal studies. Maintain accurate documentation of experiments in electronic lab notebook (ELN). Analyze and interpret data independently. Author reports and contribute to manuscripts and patents with assistance. Present research findings at group, cross-functional team and lab meetings. Acts as a technical resource within own group/project team and collaborates with other interdisciplinary functions. Required Skills &amp; Experience:&nbsp; S with 10 years post-graduate experience, M.S. with 6 years of experience, or a Ph.D. in cell or molecular biology or a related field. Experience in molecular biology, RNA techniques, and cell-based assays are a must. Previous work experience in anti-sense oligonucleotide-based target validation. Experience in cell culture techniques with variety of cells (previous work experience in the culture and differentiation of human iPSC to neuronal cell types would be beneficial). Work experience in therapeutic areas involving CNS or ocular disease highly desirable. Strong organizational and recordkeeping skills, experience using ELN is highly desirable Strong oral and written communication skills. Location(s):&nbsp;&nbsp; Stoke is located in Bedford, MA. This position is onsite with an office setting based in Bedford, MA. Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Sr Director, Commercial Insights will be responsible for generating and synthesizing insights from primary market research, competitive intelligence, and relevant secondary data to guide decision-making across the organization. The role requires strong strategic thinking, the ability to turn complex and sometimes ambiguous information into clear recommendations, and excellence in influencing senior leadership. This position will report into the Vice President, Commercial Operations. Key Responsibilities: Market Research Leadership Own the end-to-end design, execution, and interpretation of primary research across patient, caregiver, HCP and payer audiences. Ensure insights are high-quality, unbiased, and directly tied to strategic questions for pipeline and commercial launch activities. Build a structured insights roadmap aligned with key development milestones, TPP refinement, positioning, and launch planning. Competitive Intelligence &amp; External Monitoring Lead Stoke’s competitive intelligence function, including landscape assessments, ongoing monitoring of clinical, regulatory, and commercial developments, and synthesis of implications. Deliver timely alerts, threat/opportunity assessments, and scenario planning to R&amp;D, Commercial, Medical, and Executive Leadership. Partner cross-functionally to ensure a consistent, forward-looking understanding of the competitive environment. Insight Synthesis &amp; Strategic Influence Integrate primary research, competitive intelligence, and relevant secondary data into cohesive, actionable recommendations. Serve as a strategic thought partner across Marketing, Commercial Operations, Clinical, Medical Affairs, and HEOR. Shape key strategic deliverables, including TPP refinement, brand strategy, patient journey mapping, demand drivers, and launch plans. Cross-Functional Collaboration Partner closely with forecasting and analytics to ensure insights inform models and commercial planning assumptions. Work as a bridge between internal stakeholders and external vendors to ensure clarity of objectives, quality of deliverables, and value creation. Vendor &amp; Budget Management Manage relationships with research agencies and CI partners, ensuring best-in-class quality and efficient use of resources. Oversee annual insights budgets, project scopes, and timelines. Required Skills &amp; Experience:&nbsp; Bachelor’s degree required; advanced degree (MBA, MPH, PharmD, MS) strongly preferred. 10+ years of progressive experience in market research, competitive intelligence, commercial strategy, or insights generation within biopharma. Experience in rare disease, neuroscience, or genetic medicine preferred. Demonstrated success presenting insights and influencing executives and cross-functional leaders. Expert-level capability in designing and interpreting primary research. Strong command of competitive intelligence, landscape analysis, and synthesizing external signals into strategic implications. Ability to simplify complexity and craft clear, compelling narratives from disparate information. &nbsp;Excellent communication, executive presence, and stakeholder management skills. Proactive, highly curious, and comfortable operating in an emerging commercial organization with evolving needs. Familiarity with secondary data sources to triangulate insights. Understanding of forecasting inputs, commercial analytics, and launch planning frameworks. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at the Director level is $185,000 - $243,000 and at the Senior Director level is $219,000 – 292,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Commercial Insights

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly experienced and strategic Director/Senior Director of Statistical Programmer to lead and grow our statistical programming function. This role will be instrumental in supporting our expanding clinical development pipeline, including a pivotal phase 3 trial. The ideal candidate will bring deep technical expertise, leadership experience, and collaborative mindset to help drive successful regulatory submissions and clinical trial execution. The Director/Senior Director will have proven leadership ability in a fast paced, environment and interact with all levels of internal staff as well as external stakeholders, including managing external consultants and CROs. This position will report into the Executive Director, Head of Biostatistics. Key Responsibilities: Provide strategic oversight for statistical programming activities across multiple development programs. Serve as the statistical programmer functional lead for the phase 3 pivotal trial, ensure robust data and analysis workflows to support NDA/MAA submission, including integrated summaries (ISE/ISS), working closely with regulatory, biostatistics, and clinical data management teams Oversee and perform quality control (QC) review of datasets, tables, listing and figures (TLFs) generated by CROs and vendors to ensure accuracy, consistency, and regulatory compliance. Ensure adherence to CDISC standards (SDTM, ADaM, Define-XML) and regulatory agency requirements. Guide programming support for exploratory and ad-hoc analyses to generate clinical and scientific insights. Establish and implement processes to ensure quality control, traceability, and reproducibility of analyses. Develop and maintain programming standards, SOPs, and QC procedures that align with regulatory expectations. Manage vendor relationships to ensure deliverables are on time, high-quality, and audit-ready. Partner cross-functionally to support clinical study reports (CSRs), data monitoring committees, and regulatory interactions. Provide leadership in planning for future in-house statistical programming capabilities, including team building and process development as needed. Why Join Us: Opportunity to build the statistical programming function in a growing biotech. Direct involvement in advancing multiple drug candidates, including a pivotal phase 3 program. Small, agile team environment with visibility and impact at the highest levels of the company. Mission-driven organization focused on developing disease-modifying therapies for patients with high unmet medical need. Required Skills &amp; Experience: Master’s degree (or higher) in statistics, Computer Science, Mathematics, or related field. 10+ years statistical programming experience in biotech industry. Proven track record leading programming for pivotal phase 3 trials and regulatory submissions (NDA/MAA/BLA). Hands-on expertise in SAS and R programming, with strong knowledge of CDISC SDTM, ADaM, Define-XML, and FDA submission standards. Experience managing external vendor/CROs in a global clinical development environment. Experience with machine learning programming is a plus. Knowledge of AI/ML is a plus. Location(s): This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Statistical Programmer

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Formulation Scientist to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, Formulation Lead. Key Responsibilities: Design and execute formulation studies for oligonucleotide drug products Prepare prototype formulations and conduct pre-formulation characterization (e.g., solubility, pH stability, buffer selection) Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Maintain accurate and timely documentation of experiments, protocols, and results in electronic lab notebooks Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional duties as assigned Required Skills &amp; Experience: PhD in Pharmaceutical Sciences, Chemistry, Biochemistry, or related field, Master’s degree with 6+ years of experience in formulation development, or Bachelor’s with 10+ years of experience Experience with nucleic acid-based therapeutics preferred Familiarity with oligonucleotide properties, degradation pathways, and formulation challenges Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Scientist I, Formulation

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Director, Regulatory Writing and Operations oversees the strategic direction and management for both the Regulatory Writing and Regulatory Operations functions within Stoke.&nbsp; This position is responsible for the preparation, collaborative authoring, and completion of ICH-compliant regulatory documents, including those for clinical. Additionally, this position oversees submission and document management, publishing, and the Regulatory Information Management System (RIM). Demonstrates excellent understanding of drug development and leadership behaviors consistent with level.&nbsp; This position will report into the Chief Regulatory Officer. Key Responsibilities: Partners with stakeholders to author complex regulatory documents including but not limited to; clinical study protocols, clinical study reports, investigator’s brochures, regulatory briefing packages, regulatory responses, and sections of marketing authorization applications. Develop and manage SOPs, editorial standards, internal templates, and style guides. Partner with peers to increase visibility of upcoming submissions and deliverables across the regions. Work directly with team members to communicate and drive timelines, lead document review and roundtable discussions. &nbsp; Provide expert review of study-level and program-level documents for consistency in messaging and formatting. Effectively manage workloads and product quality of internal and contract medical writers. Direct the development and implementation of standards (SOPs and work processes) for dossier development, management, publishing, archiving, metrics, and submission process. Accountable for QC and validation of submissions to ensure they are error-free from a regulatory validation standpoint and are submitted on time to meet any internal or external requirements. Oversee maintenance, validation, implementation and training on operations systems. Drive continuous process improvement and increased communications across regulatory through leadership of initiatives to capture learnings, integration of new tools and technologies, and identify opportunities for change. Lead or participate in process improvement or operational excellence initiatives within Regulatory function or Stoke in general, as applicable.&nbsp; Required Skills &amp; Experience: In-depth knowledge of industry requirements and best practices policies for clinical regulatory documents (e.g., GCP, ICH). Sound working knowledge of writing, publishing and RIM computer applications Excellent written and oral communication skills. Ability to manage teams and individuals. A BS/BA degree or equivalent, and a minimum of 10 years’ experience in Medical or Regulatory Writing. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Proven ability to lead complex projects, with flexibility and adaptability. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $262,000 - $299,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Regulatory Writing and Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Senior Scientist will work collaboratively across the Research organization to support preclinical efforts in understanding the target engagement, pharmacology, and efficacy of novel compounds in disease models. This position requires a scientist with experience designing and executing thoughtful in vivo studies and developing assays to establish and investigate preclinical biomarkers in tissue samples. This position will report into the Executive Director of in vivo pharmacology. Key Responsibilities: Support the in vivo characterization of novel compounds by designing and overseeing in life studies and performing molecular analysis with a combination of internal and outsourced resources. Work collaboratively across functions to support programs through preclinical proof of concept and candidate selection to advance the company pipeline. Use a high level of scientific judgment to adapt and/or create standard methods and techniques by applying breadth of knowledge and prior work experience. Develop and optimize preclinical biomarker assays to support discovery efforts and bridge into early development activities. Maintain excellent and timely recordkeeping while adhering to aggressive timelines Author study reports that may be used for regulatory filings and scientific manuscripts. Required Skills &amp; Experience: Bachelor’s degree with 16+ years of experience, or Master’s degree with 10+ years of post-graduate experience, or Ph.D. with 6+ (industry experience is a plus). Degrees in Pharmacology, Neuroscience or relevant disciplines highly preferred. Experience working in drug discovery with an emphasis on neurology indications, experience working with oligonucleotides is a plus. Designing and managing experiments at contract research organizations. Experience developing and optimizing assays in tissue matrix (i.e MSD, ELISA, qPCR), IHC skills a plus. Excellent verbal and written communication skills. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): Stoke operates in Bedford, MA.&nbsp; This position is onsite with an office based in Bedford, MA. Travel: This position will require approximately 5% travel.&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: The Vice President, Global Medical Affairs, will be an individual contributor role with key strategic leadership responsibilities to inform and guide clinical, medical affairs and commercial work across genetic developmental and epileptic encephalopathy (DEE) indications. This individual will serve as a medical expert, building and maintaining strong relationships with internal functions and with external key opinion leaders, healthcare professionals, and patient advocacy groups. They will collaborate actively with clinical development and biostatistics in the design of clinical studies and actively guide analysis and interpretation of data to ensure the safe and effective use of our therapies, generating real-world evidence, and contributing to the overall success of our mission. This position will report into SVP Global Medical Affairs. Key Responsibilities: Collaborate closely with Medical Affairs Field Medical team to establish and maintain strong scientific relationships and collaboration with key experts and investigators in pediatric developmental and epileptic encephalopathies. Plan and execute ongoing advisory and strategic working groups to gather expert advice to guide strategic decision-making and medical affairs work. Collaborate closely with clinical development to engage with clinical experts to guide clinical study design and execution of clinical trials. Represent Stoke in presentation of clinical data at scientific conferences, medical meetings, and other external events. Provide medical expertise in close collaboration with clinical development, biostats, HEOR and medical affairs to critically interpret clinical data and define product profile. Assist medical communications and investigators in the development and publication of important findings to advance clinical and scientific understanding in defined areas. Provide medical expertise and support to internal stakeholders, including commercial, clinical development, regulatory, and market access teams. Contribute to the development of medical communication and education materials, including scientific abstracts, manuscripts, and slide decks. Provide expertise in delivering medical training to internal staff in support of onboarding and ongoing education. Facilitate interdisciplinary collaboration to enhance patient experience, including working with non-physician providers, nurses, pharmacists, office staff, and therapists. Ensure compliance with all relevant regulatory guidelines and ethical standards in medical affairs activities. Monitor and analyze the competitive landscape and emerging scientific trends in pediatric epilepsy. Required Skills &amp; Experience: MD/DO degree required. Board certification in Neurology, Pediatrics, or a related specialty is highly preferred. Minimum of 10-15 years of combined clinical and pharmaceutical or biotechnology industry, with a significant focus on pediatric epilepsy or rare neurological diseases. Deep clinical experience and expertise in pediatric epilepsy is essential. Excellent communication, presentation, and interpersonal skills, with the ability to effectively engage with diverse stakeholders. Demonstrated ability to build and maintain strong relationships with key opinion leaders and external experts. Thorough understanding of regulatory requirements and ethical guidelines governing medical affairs activities. Ability to travel globally as required. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is remote position. Travel: This position will require approximately 50-70% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Global Medical Affairs - Pediatric Neurology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The ED, ZOR Marketing will lead development of pre-commercial marketing activities, including evolution of a recently-launched disease awareness campaign and efforts to enhance genetic testing in Dravet Syndrome. The incumbent will also be responsible for developing a launch strategy and tactics in Dravet Syndrome.&nbsp; This position will report to the Vice President of Marketing. Key Responsibilities: Develop and manage impactful brand and marketing communications. programs, which support key initiatives and market development efforts. Plan and implement campaigns which integrate all appropriate marketing tactics, including digital, interactive and online media, mobile applications, and other new methods that augment traditional marketing tactics. Identify and turn physician/brand insights into actionable programs that have a beneficial impact on the growth of the business. Support key business functions across the business (e.g. DTC campaign, patient access programs, speaker bureau etc.). Creates the execution of a Global Marketing Strategy as well as action plans for sales force aligned activities. Proactively collaborate and strategize with Commercial teams to ensure alignment and develop regionally relevant, robust marketing and promotional programs. Develop ZOR positioning, messaging platform and corporate brand architecture. Oversee and manage creative agencies. Manage and approve expenditures within budget guidelines. Communicate brand and marketing strategy to the organization, ensuring consistency across all customer touch points. Scope and incorporate appropriate voice of customer research and insights into campaign development and decision-making process. Develop and champion Stoke’s brand to ensure it is consistently and meaningfully represented by all internal stakeholders, across all customer touch points. Develop and/or evolve department operational procedures and processes that foster effective communications. Establish metrics and track program effectiveness and ROI.&nbsp; Required Skills &amp; Experience: Bachelor's degree required. Master’s degree preferred. Minimum of 12 years of experience required, with 5+ years of marketing executive experience in the pharmaceutical, biotech, life science, medical device or general healthcare industries. Solid acumen around all functions that are integral to successfully evaluating potential opportunities (R&amp;D, regulatory, commercial, financial, manufacturing, legal, etc.). Experience with rare disease product launch. Proven experience managing complex marketing operations processes (Promotional Material Review, Brand Planning, Market Research, CRM Management etc.). Familiarity with marketing automation principles and marketing automation platforms. Strong understanding of related aspects of marketing concepts and principles; patient and consumer marketing. Possess the ability and desire to measure and track performance and success of programs/initiatives. Excellent interpersonal relationship skills including negotiating and relationship management skills with ability to drive achievement of objectives. Ability to manage competing priorities in a fast-paced environment. Experience in managing external agencies and vendors and managing budgets. Proven successful project management skills; strict attention to detail. Drive and enthusiasm for operational improvements. Ability to work in an ambiguous environment and successful track record in building brand teams from the ground up. Excellent communication and people skills. Successful experience in a fast-paced entrepreneurial environment. Marketing and management experience at the VP level Strength in managing people leaders.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a remote position with an office setting based in Bedford, MA location. Travel: This position will require approximately 30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $242,000 - $308,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director, Zorevunersen Marketing

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Medical Director, Neurology Clinical Development will play a key role in the planning, execution, and interpretation of clinical studies focused on transformative therapies for life-threatening neurological diseases. This individual will contribute medical and scientific expertise to advance programs from early development through late-stage studies and regulatory submission. The Associate Medical Director will support cross-functional teams, interface with external partners and investigators, and help ensure the highest standards of scientific and clinical rigor are maintained across all programs. This role will work collaboratively with the Medical Director and Senior Medical Director in Neurology Clinical Development and will report to the Medical Director in Neurology Clinical Development. Key Responsibilities: Clinical Development &amp; Execution Support the design, implementation, and oversight of clinical trials in neurology, including protocol development, study conduct, and data analysis Contribute to the medical and scientific content of key clinical documents (e.g. protocols, investigator brochures, clinical study reports, and regulatory submissions) Participate in clinical data review, interpretation, and presentation to support timely and informed decision-making Collaborate with safety/pharmacovigilance on safety review and signal evaluation Provide medical monitoring and clinical oversight for ongoing studies under the supervision of the Medical Director or Senior Medical Director Maintain effective collaboration with CROs, investigators, and academic partners to ensure study quality and adherence to timelines Ensure compliance with GCP, ICH, and all applicable regulatory and ethical guidelines Scientific &amp; Strategic Contribution Support the development of clinical development strategies and study designs in alignment with program objectives and scientific rationale Contribute to the development, execution, and continuous refinement of global Clinical Development Plans (CDPs) Integrate emerging data, literature, and competitive intelligence to inform ongoing program decisions and ensure scientific relevance and rigor Collaborate closely with cross-functional partners including regulatory, clinical development operations, and biostatistics to ensure consistency and accuracy in study execution Participate in internal discussions and presentations to communicate key scientific insights, data interpretations, and development updates Contribute to the preparation and review of strategic and regulatory documents, including target product profiles, clinical sections of IND/CTA submissions, and briefing packages External Engagement &amp; Communication Engage and collaborate with key opinion leaders (KOLs) and academic investigators to inform study designs and strengthen the scientific foundation of development programs Represent the clinical development organization at scientific and medical meetings, advisory boards, and key investigator interactions Contribute to the authorship of abstracts, manuscripts, and scientific presentations that communicate program progress and data to the broader scientific and clinical community Leadership &amp; Collaboration Provide clinical and scientific leadership with cross-functional clinical teams, fostering an environment of accountability, curiosity, and collaborative problem solving Partner effectively with internal stakeholders across clinical development operations, biostatistics, regulatory, and commercial to ensure scientific and operational excellence in study execution Serve as a clinical development point of contact in collaborations and partnerships, helping to maintain strong relationships built on scientific integrity, transparency, and shared objectives Required Skills &amp; Experience: MD, MD/PhD, or PharmD candidates with relevant clinical development experience in neurology, rare/orphan, or genetic diseases Board certified or eligible in neurology, pediatrics, or a relevant specialty (for MDs) Minimum 5 years experience contributing to the design, conduct, or medical oversight of clinical trials (industry or academic) required Familiarity with FDA/EMA regulations and ICH/GCP guidelines Strong scientific, analytical, and organizational skills Demonstrated ability to interpret complex clinical and scientific data Excellent communication and presentation skills, with the ability to collaborate effectively across multidisciplinary teams High personal integrity, scientific rigor, and strong commitment to advancing therapies for patients with serious neurological diseases Location(s): Stoke operates in Bedford, MA. This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 20% travel (domestic and international). Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $200,000 - $235,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Medical Director, Neurology Clinical Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking an enthusiastic and highly motivated Associate Scientist to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. Key Responsibilities: Design and execute routine studies to investigate the PK/PD and efficacy of drug candidates. Perform routine to intermediate handling procedures and injections, monitor animal health, and collect tissues in mice. Perform moderately complex behavioral experiments in animal models. Support the implementation of new animal models. Demonstrate technical proficiency to investigate, identify, develop, optimize, and troubleshoot new methods/ techniques/ instrumentation to address critical project needs. Read and understand scientific literature. Analyze data, author technical reports, and document experiments in an electronic notebook. Participate and present at project team and departmental meetings. Maintain laboratory organization including equipment upkeep and ensure compliance with safety protocols. Collaborate across functional areas. Required Skills &amp; Experience: BS with 7+ years post-graduate experience or M.S. with 4+ years of experience working in a biotechnology or pharmaceutical company or contract research organization. Experience working with animal disease models. Experience with animal behavioral assays. Experience with subcutaneous and intravenous injections in rodents; intracerebroventricular injection experience a plus. Experience with molecular biology assays such as PCR, ELISA, MSD, BCA, Western Blot (or JESS). Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Experience in neuroscience a plus. Experience with ISH/IHC a plus. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $114,000 - $138,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director, Patient Advocacy &amp; Engagement will build upon and execute strategic advocacy engagement initiatives for Stoke’s Dravet syndrome, ADOA, and Syngap1 programs, ensuring alignment across key Stoke functions throughout the product lifecycle. There will be a special focus on launch readiness in the U.S. for zorevunersen (Dravet syndrome) and capacity building in ADOA and Syngap1. Reporting to the Vice President of Patient Advocacy and Policy, the candidate must have strong experience in biotechnology or pharmaceutical advocacy with a broad understanding of the business. This person must thrive in a fast-paced environment, be motivated by the patient need and operate with utmost integrity. The Director will work collaboratively with cross functional team members to increase awareness of our programs in the patient community, and bring the patient voice into the everyday decision-making, programming and employee experience. Key Responsibilities: Serve as a liaison between the company and patient community by maintaining strong, trusted relationships, proactively requesting and sharing insights, and responding to community questions and needs in a timely fashion Ensure a strong connection between employees and the patient community, including identifying innovative and compliant ways for employees to connect with patients, families and disease communities Collaborate internally and play a contributing role on cross-functional teams to create and deliver patient-facing programs and materials (i.e. clinical trial awareness, launch preparedness) Ensure timely and accurate communication of company information and updates to the appropriate patient communities Partner effectively with Product &amp; Corporate Communications, Medical Affairs and Clinical Operations to ensure timely, consistent and compliant communication and responses to external patient inquiries about our programs, including clinical trials and access Coordinate with Medical Affairs and other internal functions to review and approve patient organization funding and collaboration requests; actively contribute to policy and process design/improvements for these and other related activities Lead initiatives to ensure patient insights are brought into Stoke decision-making such as advisory boards and ongoing input into programming and messaging In collaboration with Medical Affairs, organize Stoke’s strategic engagement with patient advocacy organizations at congresses and events Support broader Corporate Affairs efforts, including employee engagement and corporate communications Develop and deliver on an annual plan aligned to corporate and department goals, including developing and tracking KPIs and metrics to measure the success of advocacy initiatives; continually innovate and elevate Stoke’s approach to patient advocacy and engagement by continuously learning and staying current with best practices Manage the patient advocacy budget and lead and oversee external partners, consultants and agency resources. Required Skills &amp; Experience:&nbsp; Bachelor’s degree, preferably in social science, science, public policy, or a health-related field 10 years of professional experience in patient advocacy in a publicly traded biotech/pharmaceutical company or health-related non-profit.&nbsp;The ideal candidate has experience in rare disease, regulatory milestones (i.e., FDA Advisory Committee meetings and approvals), KOL and Society engagement, and commercial-stage experience A thorough understanding of the pharmaceutical product lifecycle, including the pre-clinical, clinical, regulatory processes and commercial stages Established track record of building and maintaining strong, trusted relationships with colleagues as well as with non-profit and/or patient communities Demonstrated ability to create and lead successful patient advocate programs and partnerships in collaboration with internal teams and stakeholders Excellent communication skills: written, verbal and presentation delivery; A good listener Strategic thinker and problem solver; intellectually curious and perceptive; able to identify interdependencies and connections across the business; a critical thinker Comfortable in a fast-moving,&nbsp;rapidly changing&nbsp;environment; able to establish structure and process when needed; consistently prioritizes high-impact projects; calm under pressure; works with a sense of purpose and urgency Understanding of laws and regulations governing the biopharmaceutical industry&nbsp;with an understanding of regulatory bodies that impact communications and reimbursement, understanding of publicly-traded company communications requirements and rules Proven understanding of the needs of individuals living with severe and rare diseases and a demonstrated passion for supporting the patient community Holds self and others to a high standard of ethics and integrity. Maintains confidentiality, exercises good judgment and discretion Strong leadership skills with demonstrated ability to influence and collaborate across the organization and at all levels, both internally and externally Able to&nbsp;work&nbsp;independently with minimal supervision Experience working with all levels of management and consulting with key business stakeholders with an ability to influence for greater outcomes Ability to travel to key U.S. and global congresses and patient events, as needed (up to 30%) Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position (2-3 days/week) with an office setting based in Bedford, MA location. Travel: This position will require approximately 30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $231,000 - $242,000 The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Patient Advocacy & Engagement

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a dedicated and highly motivated Principal Scientist in Oligonucleotide chemistry to join a dynamic team focused on advancing oligonucleotide-based therapeutics. Within the chemistry team, you will work with talented scientists in managing the synthesis of bioactive oligonucleotides for our drug discovery programs and delivering to the project teams with strict timelines. Successful candidates will have significant experience with solid phase chemical synthesis of oligonucleotide, conjugation, HPLC purification, and LC-MS analysis. This position will report to the Director, Chemistry. Key Responsibilities: Independently design and implement scientific and synthesis strategies relating to the research and development of antisense and other synthetic oligonucleotide therapeutics. Contribute to multiple projects and provide key technical knowledge to the project team. Plan and execute solid-phase oligonucleotide synthesis for SAR campaigns on several projects. Participate in the identification, development, validation and implementation of both current and next-generation technologies to enable proprietary ASO therapeutic development including purchase and installation of hardware and software supporting synthesis, purification, and characterization of oligonucleotides. Demonstrate working knowledge of conjugation chemistry and techniques aimed at producing novel conjugates. Demonstrates understanding of the chemistry of nucleosides, nucleotides, and oligonucleotides as applied to antisense and other oligonucleotide technologies. Stay current with scientific literature and understanding of the state of the art in oligonucleotide chemistry, purification, and screening strategies. Work with both internal chemistry team and external CRO’s to generate innovative solutions to synthetic and drug design challenges within the context of oligonucleotide drug discovery projects. Authors reports and manuscripts. May contribute to patent filings and/or regulatory filing. &nbsp; Required Skills &amp; Experience: PhD in Oligonucleotide Chemistry and relevant experience in a drug discovery environment with at least 8 years of oligonucleotide synthesis experience in a drug discovery environment, Master’s degree with 12+ years of experience or Bachelor’s degree with 18+ years of experience. Strong practical knowledge of design, synthesis, and purification of synthetic oligonucleotides. Experience in scale-up synthesis would be a plus. Excellent project/time management, and problem-solving skills. Excellent written and verbal communication skills, including making effective and compelling presentations in both large and small groups and communicating complex scientific and nonscientific issues to program teams. Strong people skills and the ability to thrive in a team and goal driven environment Experience designing, synthesizing and purifying oligonucleotides to support research and scale up studies. Proven track record of scientific contributions in oligonucleotide and conjugates research as evidenced by publications/presentations/patent&nbsp;applications and/or the identification of preclinical development candidates. Location(s): Stoke operates in Bedford, MA. This is a lab-based position based in our Bedford, MA location. Travel: This position will require approximately 0% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Benefits &amp; Compensation: At Stoke Therapeutics we are proud to offer comprehensive and competitive employee benefits, including medical, dental and vision insurance; life, long and short-term disability insurance; Paid Parental Leave; a 401K program with company match, unlimited vacation time, and an Employee Stock Purchase Program (ESPP). Compensation is market competitive for the industry and directly commensurate with experience. All positions are bonus and stock eligible. Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center. For more information, visit stoketherapeutics.com or follow the company on X at @StokeTx. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director of Product and Corporate Communications will drive efforts to advance business strategy and performance through strategic, integrated communications. This individual will execute key efforts to align and advance the corporate, product and commercial stories into a cohesive and compelling narrative as Stoke prepares to commercialize its first potential disease-modifying medicine and advances a pipeline of potential new medicines. This position reports to and works closely with the Vice President of Corporate Communications and serves as a trusted partner to cross-functional stakeholders and business leadership. This is a key role that is anticipated to provide continuing opportunities for growth and development as the company succeeds. The candidate must have deep experience in biotechnology or pharmaceutical communications, preferably within publicly traded companies, a broad understanding of the business and be someone who thrives in a fast-paced environment. This person must be motivated by the patient need and operate with utmost integrity. Key Responsibilities: Supports the development and implementation of an integrated strategic communications plan to advance Stoke’s corporate and product story and to build its reputation among key external stakeholders&nbsp;&nbsp; Contributes to the day-to-day activities of the communications function, including budgeting, planning, and consultant/agency relationships&nbsp;&nbsp; Serves as a trusted advisor to business leaders, including identifying opportunities and managing high-profile situations&nbsp; Partners with cross-functional stakeholders to drive integrated communications strategies and plans to support business objectives, including data communications, corporate milestones, launch preparedness and issues management with Commercial, Medical, Clinical Development, Regulatory, Legal, Clinical Operations, Investor Relations (IR), Advocacy and others&nbsp; Works closely with the IR team on content for IR-related activities including quarterly business updates and investor events&nbsp; Engages with our collaborators on ongoing communications opportunities and milestones&nbsp; Increases Stoke’s visibility and reputation through media relations, thought leadership, web content, social media and strategic engagements&nbsp; Builds and maintains relationships with journalists and influencers, pitching story ideas, managing inbound inquiries and serving as a spokesperson&nbsp; Prepares Stoke executives for external speaking engagements and media interviews&nbsp; Ensures compelling, consistent and effective messaging; develops corporate communications materials including press releases, media materials, Q&amp;A, slide decks, talking points, website content and videos &nbsp; Drives content development and strategy for corporate digital and social channels to ensure content is compelling, timely, accurate and effective from a technology and engagement standpoint&nbsp; Supports the adaptation of corporate materials for use with other audiences including advocates, patients/caregivers, employees, clinicians, elected officials and others to ensure consistency in message&nbsp;&nbsp; Supports crisis communications and rapid response to emerging media inquiries and corporate issues, partnering with cross-functional stakeholders to ensure timely, accurate, patient-centric and strategic communication&nbsp; Analyzes and reports on learnings from communications/PR efforts and media coverage to drive effectiveness and continuous improvement&nbsp; Studies and evaluates industry trends and peer activities to identify opportunities for Stoke to engage and improve, using experience and external benchmarks to make the case&nbsp; Helps establish and codify best practices, policies and processes to meet the needs of the department and company&nbsp; Required Skills &amp; Experience: Bachelor’s degree&nbsp;&nbsp; 10+ years of experience in public relations or communications in pharma/biotech, including product communications&nbsp; Proven success within and deep knowledge of pharma/biotech industry and regulatory environment&nbsp; Exceptional written and verbal communication skills&nbsp;&nbsp; Experienced in managing complex stakeholder relationships&nbsp; Established relationships with relevant media contacts as demonstrated through a track record of successful media relations and communications campaigns&nbsp; Strong cross-functional partner; able to influence at all levels and advocate for strategy, works collaboratively with colleagues and other stakeholders; actively solicits input and feedback; flexible and responsive&nbsp; Strategic thinker and problem-solver who is willing and able to implement tactically; is intellectually curious and perceptive&nbsp; Expertise in social media strategy and content development preferred&nbsp; Experience within publicly traded companies preferred&nbsp; Works well under pressure; has excellent time- and project-management skills and deep experience leading complex, time-critical projects; can manage multiple projects and thrive in a fast-paced, rapidly changing environment&nbsp; Can confidently and effectively advise and influence senior leaders&nbsp;&nbsp; Demonstrates exceptional storytelling skills and the ability to navigate ambiguity with sound judgment&nbsp; Holds self and others to a high standard of ethics and integrity. Maintains confidentiality, exercises good judgment and discretion&nbsp; Effectively prioritizes workload; excellent organizational skills and attention to detail&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA. This position will work primarily on-site.&nbsp; Travel:&nbsp; This position will require approximately 5% travel.&nbsp;&nbsp; Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $209,000 - $243,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director of Product and Corporate Communications

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Senior Director, Medical Affairs (Great Plains Region) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) and leading or supporting clinical discussions with national and regional payers in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Serve as field medical point of scientific/clinical contact or support for national and regional payers, healthcare systems, integrated delivery networks and other managed care organizations Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned. Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Knowledge of and engagement experience with national and regional payers is a plus Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary. Location(s): Stoke operates in Bedford, MA.&nbsp; This position is remote, field- based in the continental United States, and is responsible for a regionally designated area. Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role at the Director level is $236,000 - $266,000 and at the Sr. Director level is $294,000 - $329,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Medical Affairs - Great Plains Region

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose:&nbsp; The Associate Director will be responsible for preparation of preclinical safety data packages for clinical development. The ideal candidate will have extensive experience with the design, execution and interpretation of in vitro and in vivo toxicology studies. The candidate will be responsible for coordinating and interfacing with CROs to undertake these studies, and will be accountable for the review, analysis and reporting of the safety pharmacology and toxicology data. This individual will need to work across a multi-disciplinary team to advance programs through the pipeline. The successful candidate will have extensive experience of reviewing and creating regulatory documents (INDs, CTAs, NDAs etc.) and interacting with global regulatory agencies. This position will report to the Executive Director, Head of Toxicology.&nbsp; Key Responsibilities:&nbsp; Provide leadership, technical oversight, and guidance for all nonclinical safety studies conducted to support discovery and development programs and ensure delivery of a high-quality safety package for development.&nbsp; Design and manage nonclinical safety studies, including the evaluation, interpretation an integration of safety pharmacology, toxicology and toxicokinetic information.&nbsp; Manage the preparation and presentation of nonclinical safety data for project teams, regulatory agencies, and management.&nbsp; Prepare toxicology sections in regulatory documents (IND, CTA, IB and annual reports) and aid in preparation of responses to regulatory agencies.&nbsp; Develop an optimal CRO network to support non-clinical safety studies and manage external CROs to conduct appropriate studies.&nbsp; Manage toxicology goals, priorities, resource requirements, budget, and timelines.&nbsp; Collaborate with Discovery Research, DMPK, Chemistry/CMC functions as well as with key Clinical and Regulatory stakeholders.&nbsp; Regularly assess the oligonucleotide drug development field and use best practice for safety studies.&nbsp; Represent Stoke Therapeutics toxicology with key external stakeholders, partners, and consultants.&nbsp; &nbsp; Required Skills &amp; Experience:&nbsp;&nbsp; M.S. similar in Pharmacology, Toxicology, or a relevant discipline and 12+ years of experience or PhD and 8+ years of experience in Toxicology or a relevant field&nbsp; Track record supporting drug discovery programs from target identification through development with small molecules and/or oligonucleotides&nbsp; Experience in all aspects of toxicology including general, developmental and reproductive toxicology, carcinogenicity, toxicokinetics, and safety pharmacology&nbsp; Knowledge of FDA GLP and ICH guidelines&nbsp; Experience authoring regulatory documents and interacting with FDA and ideally, EU regulatory agencies.&nbsp; Effective management of external collaborations and service providers (CROs) and ability to bring pragmatic business minded approach to balancing internal and external capabilities.&nbsp; Familiarity with oligonucleotides or messenger RNA therapeutics&nbsp; Experience with drug development in the CNS and/or eye is a plus&nbsp; Strong organizational and recordkeeping skills.&nbsp; Strong presentational skills to be able to communicate to both internal and external audiences.&nbsp; Highly motivated cross-functional team player with the ability to work in a fast-paced environment. Ability to provide novel, innovative solutions to challenging problems.&nbsp;&nbsp;&nbsp;&nbsp; Location(s):&nbsp;&nbsp; Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office based in Bedford, MA location.&nbsp;&nbsp; Travel:&nbsp; This position will require approximately 10% travel.&nbsp;&nbsp; Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $189,000 - $258,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, Toxicology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Stoke is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights Stoke’s initial focus for its TANGO platform is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Stoke has identified STK-002 as a clinical candidate for the potential treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. The company is also pursuing a potential new medicine for Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;Position Purpose: We are seeking a highly motivated and detail-oriented Senior Research Associate to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, formulation lead. Key Responsibilities: Support the design and execution of formulation studies for oligonucleotide drug products Perform laboratory experiments in a safe and efficient manner and record research findings in ELN Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional tasks as assigned Required Skills &amp; Experience: B.S. with 4 years of experience or M.S. with 2 years of experience in Chemistry or Organic Chemistry Prior industry experience&nbsp;in oligonucleotide formulation and scale-up is desired Sound knowledge of analytical chemistry and basic nucleic acid chemistry is preferred Experience with UPLC, LCMS and formulation characterization instrument is desired Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $99,000 - $108,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging Committee (DIB) underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking a highly motivated and experienced Executive Assistant to join a company pioneering genetic disease treatments. This is a pivotal executive support role for someone who brings composure, precision, and a genuine commitment to enabling leadership excellence at the highest level. The successful candidate will provide direct support to the Chief Patient Officer, and SVP, Head of Global Medical Affairs. Key Responsibilities: Provide proactive, comprehensive, and confidential administrative support to the heads of the Commercial and Medical Affairs organizations and their teams, ensuring seamless coordination across calendars, meetings, and business priorities. Partner with the executives’ direct teams as needed to facilitate communication, scheduling, and information flow—providing occasional administrative support to ensure alignment and efficiency across functions. Manage complex domestic and international travel logistics, including itineraries, accommodations, and expense reconciliation. Find cost effective solutions while maintaining high standards for travel and events for the entire team Plan offsite activities by assisting in agenda creation, coordinating internal and external speakers, and partnering with IT for technical event needs Manage travel, expenses, meeting planning and invoicing for team events Coordinate and support complex meetings with internal stakeholders and Key Opinion Leaders, investors, and other external parties. Prepare and organize meeting materials, including agendas, presentations, and follow-up documentation for internal and external stakeholders Arrange or set-up AV for hybrid, remote, or in-person meetings and trouble-shoot basic AV technology issues for conference calls and interface with IT and conference services for advanced matters. Process and reconcile expense reports promptly and accurately. Serve as a liaison and travel coordinator for departmental visitors. Create, manage and track purchase orders as requested. Provide cross-coverage and proactive collaboration within the administrative team. Assist the administrative team on company-wide projects as requested. Handle additional special projects as assigned. &nbsp;&nbsp; Required Skills &amp; Experience: Bachelor’s degree required with a minimum of 8+ years relevant experience. Experience working in a Biotech environment preferred. Extensive experience managing executive travel. Proficiency in MS Office with expertise in Microsoft Word, PowerPoint and Excel. Detail oriented, flexible and comfortable working in a fast-paced office environment. Exceptional verbal and written communication skills. Superior organizational skills and dedication to completing projects with quality and in a timely manner. Prior experience working with field teams preferred. Must be a solution-oriented team player. Must possess forward thinking skills, problem solving, creativity and critical thinking skills. Ability to maintain high level discretion and confidentiality.&nbsp;&nbsp; Location(s): This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $109,000 - $132,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Vice President (VP) of Clinical Operations is a strategic executive responsible for providing leadership, oversight, and direction for all global clinical trial operations. Reporting to the Senior Vice President (SVP), Clinical Development Operations, the VP will drive the successful, timely, and cost-effective execution of the clinical trial portfolio. This role leads a high-performing Clinical Operations organization and partners closely with other functions to ensure studies are conducted in accordance with Good Clinical Practice (GCP), regulatory requirements, and the company’s strategic goals. The VP will also serve as the deputy for the SVP, Clinical Development Operations, providing executive-level support, representation, and continuity of leadership as needed. Key Responsibilities: Provide operational leadership for Clinical Operations across all programs, from study planning and start-up through execution and close-out. Oversee selection, performance and governance of CROs, external vendors and service partners, ensuring consistent alignment with company standards, timelines, quality expectations, and budgets. Partner cross-functionally with leaders across Clinical Development Operations, Regulatory Affairs, Medical, Biostatistics, Medical Affairs, and other key functions to drive integrated program delivery. Provide oversight of the framework for defining, reviewing, and acting on key performance indicators (KPIs) and risk management plans, ensuring proactive issue escalation and alignment between Clinical Operations, Clinical Outsourcing, and Quality. Build, mentor, and empower a high-performing Clinical Operations organization, fostering accountability and professional growth. Lead or contribute to regulatory submission strategies (e.g., NDA, BLA, MAA) and provide operational leadership during inspections and health authority interactions. Champion a culture of quality, operational excellence, and collaboration across the organization. Oversee the implementation of best practices and industry standards in clinical operations, maintaining up-to-date SOPs and continuous process improvement initiatives. Support the SVP, Clinical Development Operations in strategic planning, governance meetings, and executive decision-making; act as deputy when required. Required Skills &amp; Experience: Bachelor’s or advanced degree in Life Sciences or a related field, with approximately 20 years of progressive experience in Clinical Operations, including at least 3 years leading a Clinical Operations department. Prior experience in rare disease, pediatric, gene therapy, or other high-complexity indications preferred. Demonstrated success in delivering complex global clinical programs across multiple therapeutic areas and phases. Experience building or scaling the clinical operations function from early-stage to late-stage programs in a biotech setting. Familiarity with global trial execution across US, EU, Japan, emerging markets and with multi-region regulatory submission strategies. Experience implementing clinical operations systems (CTMS), process improvements and change management. Direct experience contributing to regulatory submissions and marketing authorization applications (e.g., NDA, BLA, MAA, or equivalent). Experience with regulatory inspections (FDA, EMA, PMDA, or other authorities), including preparation and post-inspection follow-up. Deep understanding of ICH GCP, FDA, EMA, and global regulatory requirements. Strong vendor/CRO oversight, budget management, and strategic execution expertise. Exceptional communication, leadership presence, and strategic decision-making skills. Ability to thrive in a fast-paced, dynamic, and highly collaborative environment. Location(s): Stoke operates sites in Bedford, MA. This position is hybrid/remote based position that will require a consistent presence in our office at least 3 days a week. Travel: This position will require approximately 15% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $311,000 - $374,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Clinical Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Reporting to SVP of Translational DMPK and Clinical Pharmacology, this leadership role will be responsible for providing strategic and scientific leadership for DMPK and biomarker discovery and development to support the programs from early discovery through clinical development. Key Responsibilities: To ensure that DMPK/ADME and biomarker data inform key decisions to support regulatory submissions. Drive the DMPK strategy for new programs and support the functional leads for INDs/CTAs. Oversee related authorship and review of key regulatory documents and engagement with global health authorities. Provide thought leadership and decision-making support on IND readiness. Collaborate with clinical development team and author and review the relevant sections of IBs, clinical study protocols, CSRs and NDAs/BLAs. Contribute on and provide leadership to discovery-stage project teams as a subject matter expert, providing PK and PK/PD input to the design of preclinical studies and predict clinical outcomes. Collaborate with R&amp;D team and toxicologist to guide dose selection in preclinical studies, dose/exposure rationale, and safety margins. Ensure that appropriate guidance from regulatory agencies is utilized to support GxP work overseen by DMPK team. Direct in vitro and in vivo DMPK studies, to enable and inform clinical modeling and translational assessments. Lead the integration of biomarker strategies into preclinical and clinical programs to inform drug response, PK/PD modeling and potentially patient stratification. Lead integration of bioanalysis, toxicokinetics, and potentially biomarker strategy into nonclinical programs to inform clinical development and regulatory submissions. Collaborate with the SMEs and mentor scientists in DMPK team. Identify new and manage existing consultants to support DMPK studies. Maintain or develop expertise in evolving clinical pharmacology and pharmacometrics approaches. Assist in forecasting resources and the department budget. Represent the company in external scientific forums, collaborations, and regulatory interactions. Excellent communication skills, including the ability to present complex scientific concepts and make actionable recommendations to the team. Required Skills &amp; Experience: PhD in a scientific discipline with 18+ years of work experience in academia and industry, that is related to drug discovery and development. In depth understanding of DMPK and proven track record of leading DMPK strategies. Background in developing and implementing biomarker strategies to inform drug development across preclinical and clinical stages is essential. Established in leadership role overseeing teams and representing the function to both internal stakeholders. Demonstrated track record of advancing oligonucleotide therapeutics-based programs from early discovery through clinical development and regulatory filings. Knowledge of FDA/EMA/ICH guidelines to support GxP work in DMPK. Experience in overseeing CROs, external consultants, and working with cross-functional program teams. Strong leadership, team building, and communication skills; ability to guide functions and levels. Experience in working with therapies targeting neurology is desirable.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $330,000 - $365,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, DMPK & Biomarker Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach. Position Purpose: The Executive Director, Clinical Development Operations Excellence and Compliance, is a senior operational leader responsible for elevating the efficiency, quality, and compliance of clinical development operations execution. This role focuses on optimizing operational performance, embedding compliance by design, and ensuring global consistency in processes, systems, and governance frameworks across Clinical Development Operations and Clinical Development. This role will drive operational excellence, department risk mitigation, and continuous process improvement to ensure clinical programs are delivered with speed, quality, and regulatory integrity. Operational Excellence is about embedding an innovative strategy to set Stoke on a path of continuous improvement.&nbsp; It is a focused long-term change and involves every member of the Clinical Development Operations organization, at every level. This position will report into the Senior Vice President, Clinical Development Operations and will be part of the department’s leadership team. Key Responsibilities: Partner with functional leaders to refine and optimize processes that elevate operational excellence and compliance across clinical trials, ensuring efficiency, consistency, and high-quality delivery. Establish and maintain harmonized, scalable processes and other solutions that improve executional quality and cycle time. Integrate regulatory and GCP requirements into operational practices to ensure proactive compliance and inspection readiness. Identify, prioritize, and lead initiatives that strengthen operational capability and organizational learning across clinical development operations. Collaborate with Clinical Development, Regulatory, Quality, and other applicable functions to align processes, mitigate operational risk, and support portfolio execution. Build/connect interface between SOP/process owners and functional leaders across Clinical Development Operations and Clinical Development as needed. Champion digital innovation, process simplification, and change management to modernize how clinical trials are delivered. Participate in governance committees related to operational excellence, clinical compliance, and risk management. Oversee internal audit readiness for Clinical Development Operations and support inspection management across global trials; support Clinical Study Teams with proactive risk mitigation and CAPA implementation as needed. Implement and lead an oversight and monitoring strategy to ensure consistent visibility into clinical execution quality, operational risk, and compliance performance across programs. Establish and lead a Clinical Development Operations Compliance Committee with managers and key study leads to review study quality, deviations, and regulatory compliance. Required Skills &amp; Experience: Degree in Life Sciences, or a related field with 18+ years of experience in Clinical Development Operations or Clinical Operations, with at least 10 years in a senior operational or compliance leadership role. Deep understanding of global clinical research regulations (ICH-GCP, FDA, EMA, PMDA, etc.). Proven experience driving operational excellence and quality improvement initiatives in a complex, matrixed environment. Strong track record in inspection readiness, audit management, and remediation planning. Exceptional leadership, communication, and stakeholder management skills. Demonstrated ability to influence at the executive level and drive cross-functional alignment. Location(s): Stoke is in Bedford, MA.&nbsp; This hybrid position is based at Stoke’s Bedford, MA office, with a minimum on-site requirement of two days per week. Travel: This position will require approximately 25% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $286,000 - $322,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP). Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director, Clinical Development Operations Excellence and Compliance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Manager, Program Management is a flexible execution-enablement role within the Program Management function, designed to strengthen program management capability, infrastructure, and day-to-day execution support across internal teams. The Senior Manager plays a critical role in driving program success by partnering closely with the lead Program Manager and the extended Program Team. Acting as a PM force multiplier, this role delivers hands-on project management support, strengthens PM ways of working, and builds scalable tools and processes by providing targeted expertise where it’s most needed to enable integrated planning and team cohesion This role offers significant growth through exposure to alliance program execution, cross-functional development work and PM capability building in a fast-moving biotech environment. This position plays a pivotal role in enabling sub-teams and external partners to operate as high-performing execution engines, complementing the lead Program Managers’ integration and strategic Program Team leadership to ensure seamless delivery and alignment&nbsp; This position will report into the Director, Program Management/Program Team Lead PM. Key Responsibilities: Project Management Enablement &amp; Sub-team Support Provide hands-on project management support to sub-team leads and sub-team managers to strengthen planning, tracking, and execution. Support teams in applying core PM disciplines (e.g., work planning, milestone tracking, issue escalation, dependency awareness). Act as a trusted PM advisor to teams that do not have dedicated PM capacity, helping improve consistency and execution rigor. Execution Tracking &amp; Risk Awareness Support tracking of milestones, actions, risks, and issues as needed across the sub-team and Program Team (in partnership with the Lead PM). Proactively surface execution risks, gaps, or misalignment to the Program Manager for integration and escalation. Help teams prepare clear inputs and updates as needed, to support efficient Program Team and governance discussions. PM Capability and Infrastructure Building Build, maintain, and continuously improve program management infrastructure including timelines, trackers, dashboards, templates, SharePoint sites and reporting tools, while contributing to the evolution of the function through scalable tools, templates, and processes. Support consistent application of PM standards, tools, and best practices across sub-teams and alliance workstreams. Ensure data quality, version control, and clarity of ownership across PM artifacts. Support onboarding and enablement of team members into PM processes and tools. Communication, Synthesis &amp; Clarity Draft clear, concise written materials (e.g., summaries, trackers, updates, pre-reads) that enable efficient review and follow-up. Facilitate clear communication across sub-teams and working groups, ensuring decisions, actions, and next steps are well understood. Bring structure and clarity to ambiguous or evolving execution challenges. Required Skills &amp; Experience:&nbsp; Required Bachelor’s degree (preferably in a scientific, engineering, or related field); advanced degree a plus. 3–5+ years of experience in project or program management within biotech, pharmaceutical, or life sciences organizations. Experience supporting cross-functional Strong written and verbal communication skills, with the ability to synthesize information and drive clarity. Demonstrated ability to operate flexibly, manage multiple priorities, and adapt to evolving needs. Experience in building PM capability, tools, and ways of working. Proficient in PM tools such as MS Project, SmartSheets, OnePager, etc. Preferred Familiarity with clinical development processes and development governance. Experience supporting working in alliance-based or partner-driven development models. PMP or equivalent certification (or progress toward certification). Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $174,000 - $202,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, Program Management

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Vice President, Distribution &amp; Trade – Market Access Field will be responsible for designing, implementing, and managing Stoke’s specialty distribution strategy for its first potential commercial product. This role will ensure seamless patient access, compliant operations, and a best-in-class distribution model tailored for zorevunersen. Reporting to the VP of Market Access, this individual will serve as the company’s lead expert on trade and channel distribution, overseeing partnerships with specialty distributors (SDs), specialty pharmacies (SPs), and third-party logistics (3PL) providers. The ideal candidate brings deep operational experience in specialty product distribution, rare disease commercialization, state licensing, and DSCSA serialization. Key Responsibilities: Distribution &amp; Trade Strategy Design and execute the U.S. specialty distribution and trade strategy aligned with the product access and commercial plan. Establish and manage relationships with channel partners (SDs, SPs, 3PLs) to ensure timely, compliant product distribution. Lead selection, contracting, and performance management of all trade and distribution vendors. Develop and monitor SLAs, KPIs, and reporting to ensure optimal service levels and inventory management. Operational Execution Oversee the operational readiness of the distribution network to support launch, including order management, returns processing, and product traceability. Manage day-to-day trade operations, including data flow, order-to-cash processes, and performance reviews with distribution partners. Ensure DSCSA serialization and traceability compliance across the distribution network. Oversee state licensing activities related to product distribution and ensure all licenses are obtained, maintained, and compliant with federal and state regulations. Cross-Functional Collaboration Partner closely within Market Access and across Finance. Supply Chain, Quality, Regulatory, and Patient Services to ensure an integrated, compliant, and patient-centered distribution model. Collaborate with Supply Chain and Quality to align on product release, distribution, and logistics. Support Finance and Legal in contract negotiation, distribution fee structures, and compliance with fair market value standards. Compliance &amp; Governance Ensure all trade and distribution activities comply with DSCSA, PDMA, GDP, and other applicable regulations. Develop and maintain standard operating procedures (SOPs) for distribution, product handling, and data integrity. Collaborate with Compliance and QA functions to support audits and ongoing monitoring.&nbsp; Required Skills &amp; Experience:&nbsp; 10+ years of experience in U.S. pharmaceutical/biotech trade, distribution, or channel operations; prior experience in rare disease or specialty biologics Bachelor’s degree in business, Supply Chain, Life Sciences, or related field (MBA preferred). Proven track record launching specialty products and managing limited distribution networks. Deep understanding of DSCSA serialization, state licensing, GDP, and 3PL operations. Start-up Experience - Ability to work hands-on, build from scratch, and manage multiple vendors and consultants in a resource-limited environment. Strong vendor management and negotiation experience. Excellent project management and communication skills. Analytical mindset with strong attention to operational details. Passion for rare disease and improving patient access. Location(s): Stoke’s headquarters is located in Bedford, MA.&nbsp; This position is a field-based position. Travel: This position will require approximately 30%-50% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $333,000 - $375,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Distribution & Trade – Market Access Field

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Manager, GCLP Compliance, is responsible for overseeing all aspects of compliance for GCLP testing for Stoke’s clinical development programs with Stoke’s Contract Research Organizations (CROs) partners. This person will be a key contributor in growing and shaping Stoke’s GCLP quality monitoring program and will be responsible for supporting Stoke’s DMPK study team towards ensuring data quality and compliance with regulations (e.g., FDA, EMA, etc.). This candidate will be required to contribute to the establishment and maintenance of Stoke’s Quality Management System for the areas of GCP and GCLP. This person will partner with Stoke’s Quality, DMPK and clinical study teams to create a quality culture within Stoke and help reach a sustained state of inspection readiness. This role requires the person to identify and effectively communicate compliance risks and drive action plans to mitigate risks. This role will report into the Director of Clinical Quality and will interface closely with other cross-functional groups such as DMPK, Clinical Development, Clinical Operations, Regulatory and other functions with our vendors and across the Stoke organization. The ideal candidate will have a strong working knowledge of relevant GCP/GCLP FDA, EU, ICH guidelines, and regulations and experience working with CROs and vendors. This candidate should have experience in auditing and a demonstrated ability to communicate significant observations in a sound and factual manner. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for quality management is essential to this role. The candidate should be willing to roll up their sleeves and directly contribute to the implementation of processes and resolution of issues as required. Key Responsibilities: Contribute to the development of procedures to oversee the quality component of Stoke’s GCLP/ Bioanalytical work at the vendors. Provide support with vendor qualification and management. Partner closely with members of the DMPK and Clinical Development Operations teams to support the successful and compliant execution of GCLP bioanalytical work to meet regulatory requirements, guidelines, internal policies, and procedures. Collaborate with Subject Matter Expert’s (SMEs) to develop, maintain and report on metrics from quality monitoring plans. Support internal, in-process, study, and process audits related to GCLP activities and bioanalytical studies. Oversee and assist with audits of vendors for GCLP Bioanalytical laboratories, as necessary, and work with Stoke’ technical SMEs staff to ensure the implementation of appropriate CAPAs as applicable. Support investigations into GCLP non-compliance. Partner with CROs and the DMPK and clinical study team to analyze investigation findings to identify root/probable cause. Ensure adequate investigation, documentation, and implementation of appropriate CAPAs. Assure timely reporting of potential or confirmed violations, as appropriate, to regulatory agencies. Represent QA on project teams, as needed and provide GCLP compliance interpretation, consultation, training, and other supportive services necessary to maintain and improve the quality of research for timely regulatory submissions. Help train/prepare Stoke employees for regulatory inspections. Provide input to and review of responses to regulatory agency questions resulting from regulatory inspections. Contribute to the development of Stoke’s Quality culture. Required Skills &amp; Experience: BS. in Biochemistry, Chemistry, Biology, Pharmaceuticals or equivalent with a minimum of 6+ years’ combined experience in a GCP/GLP environment supporting the development of biopharmaceutical products. Experience in RNA based therapeutic modalities preferred. Fully versed in GCP/GLP, ICH Guidelines, FDA, and European regulatory agency requirements (understanding of other regulatory guidelines for other countries a plus). Experience building systems from scratch and scaling processes. Practical knowledge of GCLP related to audits and business standards. Attention to detail with the ability to work within a multi-disciplinary team, as well as with external partners and vendors to establish and maintain a strong quality relationship. Ability to make decisions that are guided by applicable regulatory mandates, Stoke and designated vendor policies, procedures, and business plans. Ability to work autonomously, provide QA advice, effectively manage time, and deliver results on time. Ability to collaborate effectively in a dynamic, cross-functional matrix environment. Strong verbal and written communication skills. Conflict resolution/management and negotiation skills. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 20% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $140,000 - $153,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Manager, GCLP Compliance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: We are seeking a highly motivated and detail-oriented Senior Research Associate to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, formulation lead. Key Responsibilities: Support the design and execution of formulation studies for oligonucleotide drug products Perform laboratory experiments in a safe and efficient manner and record research findings in ELN Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional tasks as assigned Required Skills &amp; Experience: B.S. with 4 years of experience or M.S. with 2 years of experience in Chemistry or Organic Chemistry Prior industry experience&nbsp;in oligonucleotide formulation and scale-up is desired Sound knowledge of analytical chemistry and basic nucleic acid chemistry is preferred Experience with UPLC, LCMS and formulation characterization instrument is desired Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $99,000 - $108,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Director of Regulatory Strategy supports the development and implementation of innovative global regulatory strategies for preclinical and early clinical development products, as assigned. This role will be responsible for collaborating across teams to provide regulatory guidance and manage regulatory submissions to meet corporate goals. Serves as a liason between internal teams and external partners to communicate regulatory plans and updates, navigate complex regulatory issues and ensure alignment across program teams/subteams. This position reports to the Executive Director, Regulatory Affairs. Key Responsibilities: Support the development and implementation of global regulatory strategies for assigned programs, ensuring alignment with corporate objectives and regulatory requirements. Lead or support the development of high-quality regulatory documents, including authoring and/or reviewing documents for IND/CTA submissions, heath authority meetings, regulatory applications (orphan, BTD, etc) and responses to health authority requests for information. Contribute to the planning and execution of successful regulatory agency meetings and interactions; may independently lead regulatory communication and interactions with the health authority. Represent Regulatory on clinical study teams, cross-functional development subteams, and program teams as assigned to provide strategic regulatory guidance and support to achieve product development goals. Contribute to the development of risk assessments pertaining to the execution, quality, safety, and efficacy aspects of programs and applications. Collaborate with internal and external stakeholders, manage and oversee third-party vendors and consultants to ensure high-quality submissions and successful execution of clinical trials and regulatory strategies. Monitors the development of new regulatory requirements or guidance documents and advises product teams of the impact on the business or development programs. Lead or contribute to process improvement or operational excellence initiatives within Regulatory function or Stoke in general, as applicable. Stay current with evolving regulatory requirements and best practices, and ensure compliance with all applicable regulations, guidelines, and industry standards.&nbsp; &nbsp;&nbsp; Required Skills &amp; Experience: Bachelor's degree in health sciences or related field; an advanced degree is preferred. Minimum of 6 years of relevant pharmaceutical or biotech industry experience within regulatory affairs. Prior experience in regulatory strategy supporting global clinical trials. Experience in rare disease drug development is highly desirable. Ability to assess and understand complex scientific information, regulatory precedents, competitive intelligence, and regulatory guidelines and make recommendations based on assessment. Excellent verbal, written, negotiation, and interpersonal skills. Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines. Proven ability to carry out complex projects, with flexibility and adaptability. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. &nbsp;&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $189,000 - $227,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, Global Regulatory Strategy

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Manager, Regulatory Project Management, through partnership with the global regulatory leader, has significant responsibility for developing, driving, operationalizing, and executing regulatory program strategy.&nbsp; This individual will serve as the primary point of contact to ensure Regulatory functional plans are aligned with program objectives and commitments.&nbsp; Works closely with the Global Regulatory Leader (GRL) and partners across Regulatory Affairs as well as Project Management, Medical Writing, Clinical and other key functions to ensure confidence, quality, and speed in the delivery of regulatory goals. This position will report to the Chief Regulatory Officer. Key Responsibilities: Partners with the program global regulatory lead (GRL) to ensure global regulatory project plans reflecting the goals of the program team and submission working group (SWG) plans are established and executed upon. Partnering with the program GRL, co-lead and facilitate regulatory subteam meetings and cross-functional SWG meetings, to oversee, plan and deliver goals in accordance with regulatory strategy. Prepare and deliver reports and metrics on major regulatory milestone status, potential critical issues, constraints, bottlenecks, regulatory risks and mitigation management for assigned programs. Collaborate with the submission team members to identify areas of improvement and implement necessary measures, including identifying and proposing solutions for addressing potential systemic bottlenecks and constraints. Operational support may include, but is not limited to drafting and preparation of forms and cover letters, providing logistical support for regulatory subteams and submission working groups, document reviews, health authority meetings, coordinating roundtable meetings, drafting regulatory notifications, coordinating and managing regulatory document workflows, inputting information into regulatory information management systems, preparing, checking and tracking regulatory data and lists etc.). Partner with Program Management to develop efficiencies by ensuring consistent approaches and development of best practices across the organization. Lead or contribute to process improvement or operational excellence initiatives within Regulatory function or Stoke in general, as applicable. Stay current with evolving regulatory requirements and best practices, and ensure compliance with all applicable regulations, guidelines, and industry standards related to regulatory submissions &nbsp;&nbsp; Required Skills &amp; Experience: 5+ years pharmaceutical industry experience with prior responsibility for project management in a pharma function Solid understanding of drug development for pharmaceuticals Exceptional attention to detail and excellent organization skills Excellent interpersonal, written, and oral communication skills Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects Creative and open-minded&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $136,000 - $159,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Manager, Regulatory Project Management

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: We are seeking a highly motivated and detail-oriented Senior Research Associate to join our Early Development team focused on oligonucleotide-based therapeutics. This role offers a unique opportunity to contribute to the design and development of innovative drug formulations at the preclinical and early clinical stages. You’ll work closely with cross-functional teams to support formulation screening, in vitro and in vivo formulation preparation, and process development for novel oligonucleotide candidates. This position will report into Senior Principal Scientist, formulation lead. Key Responsibilities: Support the design and execution of formulation studies for oligonucleotide drug products Perform laboratory experiments in a safe and efficient manner and record research findings in ELN Collaborate with analytical, process development, and manufacturing teams to ensure seamless tech transfer Support analytical testing and interpretation of data related to physical and chemical stability Contribute to the preparation of technical reports, presentations, and regulatory documentation Stay current with scientific literature and industry trends in oligonucleotide formulation and delivery Additional tasks as assigned Required Skills &amp; Experience: B.S. with 4 years of experience or M.S. with 2 years of experience in Chemistry or Organic Chemistry Prior industry experience&nbsp;in oligonucleotide formulation and scale-up is desired Sound knowledge of analytical chemistry and basic nucleic acid chemistry is preferred Experience with UPLC, LCMS and formulation characterization instrument is desired Hands-on experience with lab techniques such as pH adjustment, UV spectroscopy, endotoxin and physical property measurement; hand-on experience with HPLC/UPLC is a plus Strong organizational skills and attention to detail Excellent communication and teamwork abilities Location(s): Stoke operates in Bedford, MA. This position is a lab-based position in our Bedford, MA location. Travel: This position will require approximately 5% travel.Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Director of Learning and Development will be responsible for leading the design, execution, and management of a comprehensive learning strategy aligned with the company’s scientific and business objectives. The ideal candidate will create training infrastructure from scratch, including launch excellence frameworks and technical training for both medical and commercial teams. This position will report to the Vice President of Marketing. Key Responsibilities: Build a comprehensive training strategy aligned with business objectives to enhance knowledge and skills on SMA and zorevunersen across U.S. and global markets. Lead and oversee medical and commercial training initiatives.&nbsp; Lead development initiatives, i.e., training, coaching, learning initiatives. Lead creation and standardization of Launch Excellence frameworks from early planning through post-launch sustainment. Design and train on learning solutions grounded in adult learning theory to maximize knowledge of retention and application. Oversee development and facilitation of dynamic workshops on disease states, products, market landscapes, and contextual selling skills for diverse audiences.&nbsp; Train&nbsp;and coach leaders for key conversations that drive team performance, instilling high confidence in the performance of team members, and ensuring employees receive balanced and actionable feedback.&nbsp; Partner with executive leaders to provide strategic guidance aligning people programs to business outcomes and while ensuring high employee engagement. Lead, evaluate and monitor training programs to ensure training objectives are being met. Ensure the appropriate utilization of resources by overseeing the identification and selection of vendors as well as existing relationships and projects relating to training. Design and implement practices ensuring continuous improvement of a high-performance culture. Other duties as assigned. Required Skills &amp; Experience:&nbsp; Bachelor’s degree in business, science or related field. A master’s degree (MBA) is strongly preferred. 10+ years of experience working in the pharmaceutical or biotech industry with 8+ years of experience in sales/commercial training. Ability to lead all medical affairs and commercial trainings. Experience leading development initiatives (i.e., training programs, feedback and evaluation, collaboration, LMS systems, career development, content creation, digital training assignments, performance consulting, learning analytics and facilitation and coaching). Firm understanding and applicability of adult learning theory framework and experience with each of the adult learning modules (i.e., visual and spatial, auditory, linguistics, andragogical and experiential learning). Experience using different learning methodologies. Strong interpersonal skills with demonstrated ability to drive toward consensus. Exceptional, persuasive, written, and verbal communication skills. Willingness to present the facts and put forth hypothesis in the face of differing opinions. Excellent presentation skills with the ability to establish logical flow and story. Experience presenting to senior management. Ability to adjust to changing needs within the organization and flexibility to change priorities as needed. Ability to self-direct projects and work independently. Ability to work cross functionally with key internal stakeholders including, but not limited to Sales, Market Access, Marketing and Human Resources. Demonstrated values-based skills and strong work ethic. Pharmaceutical launch experience with an emphasis on building infrastructure and processes from scratch. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is field-based, currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 25%-30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $256,000 - $301,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Learning & Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking a highly motivated and experienced Executive Assistant to join a company pioneering genetic disease treatments. This is a pivotal executive support role for someone who brings composure, precision, and a genuine commitment to enabling leadership excellence at the highest level. The successful candidate will provide direct support to the C-Suite Executives.&nbsp; Key Responsibilities: Provide proactive, comprehensive, and confidential administrative support to the Chief People Officer, General Counsel, and Chief Financial Officer, ensuring seamless coordination across calendars, meetings, and business priorities. Partner with the executives’ direct teams as needed to facilitate communication, scheduling, and information flow—providing occasional administrative support to ensure alignment and efficiency across functions. Manage complex domestic and international travel logistics, including itineraries, accommodations, and expense reconciliation. Plan and organize the annual schedule of Board of Directors and Compensation Committee meetings, including preparation of materials and coordination of logistics. Coordinate and support complex meetings with internal stakeholders and Key Opinion Leaders (KOLs), and other external parties. Prepare and format presentations for investor, Board of Directors, and internal meetings as needed. Process and reconcile expense reports promptly and accurately. Serve as a liaison and travel coordinator for departmental visitors. Create, manage and track purchase orders as requested. Coordinate planning, catering and logistics for departmental/company meetings and events. Provide cross-coverage and proactive collaboration within the administrative team. Assist the administrative team on company-wide projects as requested. Handle additional special projects as assigned. Required Skills &amp; Experience: Bachelor’s degree required with a minimum of 8+ years relevant experience. Experience working in a Biotech environment preferred. Proficiency in MS Office with expertise in Microsoft Word, PowerPoint and Excel. Detail oriented, flexible and comfortable working in a fast-paced office environment. Exceptional verbal and written communication skills. Superior organizational skills and dedication to completing projects with quality and in a timely manner. Must be a solution-oriented team player. Must possess forward thinking skills, problem solving, creativity and critical thinking skills. Ability to maintain high level discretion and confidentiality. Location(s): This position is an onsite position based in our Bedford, MA location. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits:&nbsp; At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program.&nbsp;&nbsp; The anticipated salary range for this role is $109,000 - $132,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and&nbsp;&nbsp; equity participation.&nbsp; Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).&nbsp;&nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Senior Director, Medical Affairs - RMD (Northeast) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) and leading or supporting clinical discussions with national and regional payers in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Serve as field medical point of scientific/clinical contact or support for national and regional payers, healthcare systems, integrated delivery networks and other managed care organizations Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned.&nbsp; Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Knowledge of and engagement experience with national and regional payers is a plus Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA. This position is remote, field- based in the continental United States, and is responsible for a regionally designated area Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is Director level is $236,000 - $266,000 and at the Sr. Director level is $294,000 - $329,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Regional Medical Director - Northeast

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Senior Director, Medical Affairs - RMD (Northwest) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) and leading or supporting clinical discussions with national and regional payers in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Serve as field medical point of scientific/clinical contact or support for national and regional payers, healthcare systems, integrated delivery networks and other managed care organizations Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned.&nbsp; Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Knowledge of and engagement experience with national and regional payers is a plus Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA. This position is remote, field- based in the continental United States, and is responsible for a regionally designated area Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is Director level is $236,000 - $266,000 and at the Sr. Director level is $294,000 - $329,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Regional Medical Director - Northwest

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Senior Director, Medical Affairs - RMD (South) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) and leading or supporting clinical discussions with national and regional payers in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Serve as field medical point of scientific/clinical contact or support for national and regional payers, healthcare systems, integrated delivery networks and other managed care organizations Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned.&nbsp; Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Knowledge of and engagement experience with national and regional payers is a plus Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA. This position is remote, field- based in the continental United States, and is responsible for a regionally designated area Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is Director level is $236,000 - $266,000 and at the Sr. Director level is $294,000 - $329,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Regional Medical Director - South

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Senior Director, Medical Affairs - RMD (Southwest) will be a core member of the global medical affairs team and will help establish core medical capabilities at Stoke Therapeutics. This is a field-based role and will primarily be responsible for representing Stoke Therapeutics with a network of external clinical experts or key opinion leaders (KOLs) in a regional area, while advancing prioritized Medical Affairs activities in collaboration with cross-functional teams. The role will initially be an individual contributor role, actively engaging a defined group of Stoke clinical investigators and key opinion leaders (KOLs) in developmental and epileptic encephalopathies (DEEs) and leading or supporting clinical discussions with national and regional payers in the US primarily, while also supporting international investigator and KOL engagement as needed. Key Responsibilities: Work closely with clinical operations and development teams to support Stoke clinical study efforts, including site identification and engagement to advance enrollment while fostering collaborative research relationships with academic and community KOLs. Establish and maintain highly credible, scientific relationships with academic and medical thought leaders. Serve as field medical point of scientific/clinical contact or support for national and regional payers, healthcare systems, integrated delivery networks and other managed care organizations Work closely with internal team members to facilitate and support congress strategy at scientific meetings, including KOL engagement planning, insight gathering on key topics, and interpretative reports to share with cross functional teams. Develop, share, and discuss ongoing clinical insights with cross-functional members to ensure awareness and understanding of KOL perspectives to inform and guide strategic decision-making. Develop strategic profiles for top experts and clinical staff at centers of comprehensive care, to help identify key opportunities for ongoing education and collaboration. Develop key performance and reputation measures to ensure ongoing alignment of Stoke activities to KOL and HCP expectations and needs. Collaborate closely with other functions including clinical, commercial and PR/IR/Advocacy to engage and support external stakeholder needs through activities such as scientific education, advisory boards, investigator meetings, publications, congresses, and other activities of interest. Must be able to perform duties typically associated with a normal office environment when not traveling. All other duties as assigned.&nbsp; Required Skills &amp; Experience:&nbsp; Advanced scientific or clinical degree, with at least 10+ years in relevant clinical/scientific fields and 5+ years of Medical Affairs experience, with significant focus on field medical activities. Experience with prelaunch and rare diseases in epilepsy, neurology highly preferred. Prior track record of success and proficiency in role working directly with clinical and academic experts is required. Knowledge of and engagement experience with national and regional payers is a plus Excellent verbal and written communication skills, and exceptional interpersonal skills. Demonstrated skills in leading and mentoring others. Good judgment, ability to adapt and change in a shifting environment. A strategic, passionate, self-starter who takes initiative. Ability to work independently and function effectively as a team member. Excellent problem-solving skills. Valid driver's license and the ability to travel as necessary.&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA. This position is remote, field- based in the continental United States, and is responsible for a regionally designated area Travel: This position will require approximately 60%+ travel, including the ability to travel overnight, internationally, and occasionally on weekends. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is Director level is $236,000 - $266,000 and at the Sr. Director level is $294,000 - $329,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Regional Medical Director - Southwest

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Manager of Scientific Communications Operations will be responsible for the operational aspects of the Medical Affairs team. The lead will work with members of medical affairs and cross functional partners to ensure excellence in operational processes and tactical execution that supports the goals of the company. This position will report to the Director of Scientific Communications Operations. Key Responsibilities: Manage Medical Information systems and tools, including drafting responses, vendor management, triaging, etc. Provide project management support for key Medical Affairs initiatives, including medical congress planning, by ensuring timely and effective execution. Follow established congress framework to coordinate and execute cross-functional congress planning meetings to align objectives and activities. Support end-to-end congress operations, including medical booth planning, KOL engagement coordination, activity tracking, and onsite logistics, ensuring alignment with strategic objectives and seamless execution. Track, analyze, and report on congress performance metrics to generate actionable insights that support future congress planning and strategic decision-making. Leverage post-congress insights and performance data to identify engagement trends, uncover opportunities for improvement, and enable scalable growth of Medical Affairs. Support the MLR review process for congress-related and other medical materials, including managing submissions, referencing, version control, and coordination with internal teams and external partners. Drive continuous improvement across all Medical Affairs by identifying opportunities to enhance operational efficiency, promote cross-functional collaboration, and optimize the execution of key initiatives. Support the development, implementation, and maintenance of SOPs for core Medical Affairs functions, including congress planning, medical education, publications, and related areas. Any and all other operational responsibilities as requested Required Skills &amp; Experience: Bachelor’s degree required Strong operational knowledge of medical affairs or other similar departments 6+ years of related experience in medical affairs, clinical operations, or project management within the pharmaceutical or biotechnology industry Innovative, solution-oriented thinker Ability to work collaboratively in a highly matrixed organization Excellent verbal and written communication skills Strong problem-solving skills and the ability to adapt to changing priorities and deadlines Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office setting based in Bedford, MA location. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $175,000 - $202,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, Scientific Communications Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director/Senior Director, Marketing Operations&amp; Meetings will be a key member of the Commercial organization, responsible for enabling operational excellence across marketing, field, and event execution. This role will own critical systems and processes that ensure compliant, efficient, and high-quality delivery of marketing and commercial initiatives, including the Promotional Review Committee / Medical Legal Review (PRC/MLR) process, print and inventory management, standard operating procedures (SOPs), and conference strategy and execution. The successful candidate will combine operational precision with creative problem solving, ensuring seamless coordination across functions—including Regulatory, Legal, Compliance, Medical Affairs, and Sales—to drive the company’s strategic objectives and support launch readiness. This position reports to the Vice President, Sales &amp; Marketing. Key Responsibilities: Lead PRC/MLR Operations: Facilitate review meetings, manage agendas, track status, and ensure timely approvals for all promotional, medical, and corporate materials. Partner with Regulatory and Legal teams to ensure compliance with FDA and OPDP requirements. Oversee Veeva Vault process design, implementation, and continuous optimization. Develop and maintain PRC policies and best practices to ensure efficiency and compliance. Marketing Operations Infrastructure: Manage print and inventory systems, ensuring timely production, fulfillment, and distribution to field teams. Oversee third-party vendor relationships, including warehouse management, promotional materials production, and online ordering platforms. Establish and document business rules, workflows, and operational guidelines across marketing and sales teams. Track and report key performance indicators (KPIs) for operational effectiveness and program ROI. Meetings, Congresses, and Speaker Programs: Develop and execute the company’s annual congress and conference strategy in partnership with Medical Affairs, Marketing, and Sales. Secure exhibit space, sponsorships, and logistics for major conferences and professional society meetings. Oversee conference booth design, asset development, and on-site coordination. Support the planning and execution of promotional speaker programs and related logistics. Cross-Functional Collaboration: Serve as a key liaison between Marketing, Medical Affairs, Compliance, and Field Operations to ensure alignment and operational readiness. Champion process improvement initiatives and operational excellence across commercial teams. Drive consistent implementation of brand standards, compliance policies, and internal controls. Required Skills &amp; Experience: Bachelor’s degree required; advanced degree preferred. Minimum 10 years of marketing operations experience in the biopharmaceutical industry, with a strong understanding of PRC/MLR and commercial compliance. Proven expertise with Veeva Vault and related promotional review systems. Experience managing speaker programs, scientific conferences, or medical meetings. Strong understanding of pharmaceutical promotional regulations and best practices. Demonstrated success managing external agencies, vendors, and complex projects. Excellent communication, organization, and cross-functional collaboration skills. Ability to lead without authority and influence teams across multiple functions. Thrives in a fast-paced, dynamic, startup environment where adaptability and initiative are essential. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is field-based, currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 20% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at Director level is $230,000 - $252,000 and at the Senior Director level is $265,000 - $291,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Marketing Operations & Meetings

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Manager, Payroll &amp; Equity is responsible for ensuring accurate, timely, and compliant payroll execution and equity administration while driving process and system improvements. This is a hands-on, high-impact role that partners with the HR and Finance Teams.&nbsp; This position will report into the Vice President, Controller. Key Responsibilities: Prepare, process and validate semi-monthly payrolls in ADP Workforce Now, ensuring accuracy, timeliness and SOX-compliant documentation Prepare and process special payrolls, including RSU vesting and stock option exercises Manage multi-state processing and reporting, including state tax registrations, withholding accounts, and unemployment accounts Maintain payroll system accuracy, including earnings codes, deductions, tax configurations, and pay rules Prepare monthly payroll related journal entries and bonus accruals Drive investigations and resolve escalated disputes involving possible payroll issues/errors Maintain awareness of current legislation and practices in payroll processing and communicate with third party vendor about changes in current payroll and tax regulations and laws and policies Own year-end payroll activities, including review of forms 3921/3922 and W-2 forms Administer other payroll-related compliance requirements and reporting Upload 401K contributions to Fidelity and grants and ESPP contributions to ETrade Work with 3rd Party ETrade Administrator to ensure that employee data is accurate in equity platform Manage ESPP purchases and ensure enrollments are properly recorded for new offering periods Manage RSU vesting events to ensure appropriate tax withholding Prepare equity related reports/analyses as needed Develop, maintain, and implement policies and standard operating procedures to ensure compliance to internal controls Provide clear, prompt, professional and accurate communication to employees regarding payroll and equity questions Provide support on workers’ compensation, 401(k), and KPMG audits, and provide workers’ compensation data for insurance renewals Required Skills &amp; Experience: Bachelor’s degree in Accounting, Finance, or related field CPP designation a plus 5+ years of hands-on payroll processing experience, ideally in high-growth environments Strong experience with multi-state payroll and complex tax scenarios Proficiency with ADP Workforce Now, and experience with equity platforms (ETrade) and ERP systems (NetSuite) Experience supporting SOX, financial and compliance audits, and 401(k) plans Demonstrated ability to manage multiple concurrent deadlines with accuracy; strong sense of ownership, accountability and follow through Ability to work both independently and collaboratively in a high-growth, fast paced environment Excellent communication and interpersonal abilities Proven mindset of proactive continuous improvement Exceptional attention to detail and accuracy Commitment to maintaining confidentiality and handling sensitive information with discretion Demonstrated effective interpersonal, written, and verbal communication skills. Experience with commercial compensation a plus Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position does not require travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $124,000 to $143,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Manager, Payroll & Equity

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking a dynamic and strategic Vice President, Business Development to partner with our Chief Financial Officer in establishing and executing on Stoke’s Business Development strategy. This is a key leadership role in the identification, evaluation, negotiation, and execution of new business development opportunities. The role will lead and support various other strategic projects related to Stoke’s strategy and growth. This role will report to the Chief Financial Officer. Key Responsibilities: Work closely with the CFO to define the vision and strategy for Stoke’s business development efforts. Create growth opportunities through the identification of partnering and licensing opportunities across the company’s pipeline.&nbsp; Prepare and present the Company’s capabilities to possible collaborators. Collaborate with the CFO and senior executive team to evolve our Business Development Strategy as needs to do so arise, and to plan for and present in Executive Leadership Team meetings as needed. Manage the entire process of business development including identification, coordination of outreach and due diligence activities, facilitation of internal and external communications. Advance deal negotiations while maintaining internal alignment on proposed transaction terms. Develop and demonstrate a strong technical knowledge of Stoke’s scientific platform as well as potentially complementary external technologies, platforms, programs, etc. Work alongside the alliance management function generally and especially where changes to existing collaborations are contemplated. Continue to refine and update our Business Development 3 and 5-year strategic roadmap, including defining annual business development goals, business development and licensing success measures while aligning these plans to corporate objectives. Partner with the FP&amp;A team to evaluate transactions and optimize budget planning, financial governance, and organizational due diligence related to business development and licensing needs. Additional responsibilities as assigned. Help make Stoke Therapeutics a great place to work for all Stoke team members! Required Skills &amp; Experience: Master’s degree in business administration or Master’s/PhD in a relevant scientific discipline. Strong scientific and/or medical knowledge and experience. A minimum of 7 years of experience in Business Development. Proven track record of developing and executing strategic transactions across all stages of clinical development. A strong record of sell-side transactions (R&amp;D partnerships, licensing arrangements, joint ventures) with pharma and biotechnology companies. Excellent negotiation skills with a demonstrated history of successfully obtaining mutually beneficial agreements. Ability to understand and clearly communicate the company’s scientific platform and strategy; lead executive-level presentations. Deep understanding of the biopharmaceutical sector, current scientific/market trends and market dynamics. Strong alignment with Stoke Therapeutics’ purpose, values and mission, and a history of building strong, inspired teams. Demonstrated success in leading and working collaboratively within smaller, entrepreneurial environments and across multi-disciplinary teams. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position with an office setting based in MA location. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $306,000 – $367,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Vice President, Business Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director (or Senior Director), Human Resources will serve as the strategic HR business partner to Stoke’s growing Commercial and Medical Affairs organizations as the company builds toward launch readiness and future market expansion. Reporting to the Chief People Officer, this role will provide leadership, strategic partnership, and hands-on support across all aspects of the people agenda for these functions, including Sales, Marketing, Market Access, Field Operations, and Medical Affairs. This leader will partner closely with senior leaders across the organization—including the Chief Patient Officer, SVP of Medical Affairs, and other members of the executive and functional leadership teams—to shape and implement the people strategy that enables Stoke’s success in transforming the lives of people with genetic diseases. The Director/Senior Director will play a pivotal role in building organizational capability, evolving field-based compensation and rewards practices, strengthening talent pipelines, and fostering a culture of accountability, agility, and collaboration. While the primary focus will be on the North America-based Commercial and Medical Affairs organizations, this role will also serve as the key HR liaison to Stoke’s external partners supporting ex-U.S. regions and may flex to support other functions as business needs evolve. The ideal candidate brings deep HRBP experience in biotech commercial and medical organizations, strong business acumen, exceptional project management skills, excellent communication and presentation skills, including the ability to develop clear, compelling PowerPoint materials for leadership discussions, and a track record of building from the ground up in high-growth, mission-driven organizations. Key Responsibilities: Serve as strategic HR business partner to the U.S. Commercial and Medical Affairs leadership teams, providing counsel on organization design, leadership development, workforce planning, and capability build as Stoke prepares for launch. Partner with senior leaders to define and implement people strategies that accelerate commercial and medical readiness, ensure launch excellence, and strengthen cross-functional collaboration. Contribute to design and ongoing management of field-based compensation frameworks (led by comm ops), collaborating with Total Rewards, Finance, and Commercial Operations to ensure competitive, compliant, and performance-driving practices. Provide coaching and counsel to leaders at all levels on leadership effectiveness, organizational dynamics, and change management. Act as a project leader for key HR initiatives—applying strong project management discipline to design, lead, and implement scalable people practices (e.g., performance management, succession, leadership development, and talent planning). Leverage data and analytics to identify trends, anticipate business needs, and provide insights that drive decision-making related to talent, engagement, and organizational effectiveness. Build strong partnerships with HR team members to deliver integrated and impactful people solutions and programs aligned with business priorities. Serve as the HR liaison to Stoke’s global and ex-U.S. partners, ensuring alignment and collaboration on talent-related processes, practices, and communications. Champion progressive and innovative HR practices that enable Stoke’s culture of learning, transparency, and shared momentum. Lead and contribute to company-wide initiatives, ensuring alignment and connection across functions. Serve as a trusted advisor and role model, exemplifying Stoke’s values and leadership expectations. Required Skills &amp; Experience: Bachelor’s or Master’s degree in Human Resources, Business, or related field. 10+ years of progressive HR experience, with significant HR Business Partner leadership supporting Commercial (Sales/Marketing/Access) and/or Medical Affairs functions in biotech or life sciences. Proven success supporting commercial and medical builds in the U.S., including design and implementation of field-based compensation structures. Experience leading organizational design, talent management, change management, and leadership development efforts in a high-growth or launch-phase environment. Exceptional business acumen and ability to build trust, credibility and influence with senior leaders. Demonstrated strength in project management—able to scope, plan, and execute cross-functional initiatives with precision and follow-through. Advanced proficiency in PowerPoint and other Microsoft Office tools; able to translate complex ideas and data into executive-ready presentations and visual materials. Strong analytical mindset and comfort using data to drive insights and decisions. Manage complex employee relations issues ensuring compliance with State and Federal laws and company policies; Conduct effective, thorough, and objective investigations, as needed. Collaborative team player who embodies Stoke’s culture of partnership, tenacity, and shared momentum. Progressive, creative, and open-minded approach to people practices, with a drive for learning and high-performance. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at the Director level is $208,000 - $242,00 and at the Senior Director level is $254,000 - $289,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director/Senior Director, Human Resources

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Sr. Manager, GMP Quality Operations will be the quality contract for quality oversight of GMP operations related to process tech-transfer, scale-up, process validation and manufacture drug substance and Drug product at Stoke’s CMOs. This individual will collaborate and partner closely with CMC department to ensure day-to-day activities maintain compliance with regulations in a phase appropriate manner. This role will report to the Director of Quality and will interface closely with other cross-functional groups such as CMC Manufacturing &amp; Analytical Development, Supply Chain, Regulatory CMC and other functions within Quality. The ideal candidate will have a strong working knowledge of relevant FDA, EU, ICH GMP, guidelines, and GMP regulations and experience working with CMOs and CTLs. It is preferred that this candidate have prior experience working with external vendors and in participating directly and indirectly in regulatory inspections. It is also preferred that this candidate has direct working knowledge of unit operations as it relates to Oligonucleotide Drug Substance manufacturing and process validation. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for quality management is essential to this role. The candidate should be willing to directly contribute to the improvement of processes and resolution of issues as required. Key Responsibilities: Oversee day-to-day management of outsourced GMP operations related to Drug product manufacturing at various CMOs to ensure successful and compliant execution of GMPs to meet regulatory requirements, guidelines, internal policies, and procedures. Review and approve documentation generated at CMOs, including master batch records, deviations and investigations, process validation protocols/reports and change controls. Perform batch disposition of all GMP batches for release for further manufacturing. Compile necessary data and analyze metrics for compliance health checks for External Quality Business Review Meetings and Quality Management Review. Represent quality on project teams, as needed and provide GMP compliance interpretation, consultation, and other supportive services necessary to maintain and improve compliance to ensure product quality, data integrity and patient safety. Support internal audit and external GMP vendor audits, as necessary, and work with Stoke staff to ensure the implementation of appropriate CAPAs. Support GMP regulatory inspections as SME, as necessary, and provide input and review of responses to regulatory agency questions resulting from these inspections. Provide GMP compliance technical support during regulatory inspections at Stoke’s CMOs as necessary. Drafts and facilitates review and approval of Quality Agreements between CMOs/Suppliers and stoke, as needed Contribute to the development of Stoke’s Quality culture. Required Skills &amp; Experience:&nbsp; BS/BA, MS in life sciences or chemistry with a minimum of 8+ years’ experience in Biotech or Pharma setting with direct experience in GMP Operations as it relates to either Oligonucleotide Drug Substance manufacturing, sterile fill finish operations. Strong working knowledge of relevant FDA, EU, ICH GMP regulations and guidelines with proficiency in interpreting and implementing GMPs in a practical manner. Experience in managing CMOs. Attention to detail with the ability to work within a multi-disciplinary team, as well as with external partners and vendors to establish strong and maintain a strong quality relationship. Strong experience successfully leading event investigations, Root Cause Analysis (RCA), and CAPA Experience with drug substance and/or drug product (both preferred) development and manufacturing with proficient knowledge in a pharmaceutical setting. Ability to work autonomously, provide QA/QC advice, effectively manage time, and deliver results on time. Ability to collaborate effectively in a dynamic, cross-functional matrix environment. Desired Experience, Knowledge and Skills:&nbsp; Experience in process validation for commercial product development. Proficient in using Veeva eQMS and Veeva Vault Quality. Prior experience in participating in regulatory inspections and responding to regulatory response. Ability to manage multiple projects in a fast-paced environment. Excellent organizational, interpersonal, verbal, and written communication skills. Conflict resolution/management and negotiation skills. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 20% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is&nbsp;$171,000 to $198,000.&nbsp;The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager, GMP Quality Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director, Competitive Intelligence (CI) will be responsible for delivering actionable competitive insights to support strategic decision-making for both zorevunersen and earlier stage pipeline assets.&nbsp; The Director will partner closely with cross-functional teams, including Commercial, Medical, Clinical and Regulatory to inform portfolio and product strategy.&nbsp; This role will report into the Vice President, Corporate Strategy and Chief of Staff. Key Responsibilities: Lead end-to-end competitive intelligence for zorevunersen and pipeline assets. Develop, implement, and scale CI processes, tools, and methodologies. Partner with Program Teams and senior leadership to embed CI into brand, portfolio, and corporate strategic planning. Translate complex market, clinical, and regulatory signals into clear, actionable recommendations that inform strategic decisions. Attend key conferences to monitor competitor activity and emerging trends. Analyze diverse data sources to identify trends and business opportunities. Serve as the primary interface with external CI vendor, ensuring clarity of objectives and quality of deliverables. Own CI budgets, project scopes, and timelines. Required Skills &amp; Experience:&nbsp; Bachelor’s degree required; advanced degree (MBA, MPH, PharmD, MS) strongly preferred. 10+ years of relevant experience in pharma/biotech or healthcare consulting with a focus on competitive and market intelligence. Experience in rare disease, neuroscience, or genetic medicine preferred. Proven ability to deliver actionable insights across the product lifecycle, from early development through post-launch. Demonstrated capability to synthesize complex datasets into clear, executive-ready recommendations that influence strategic decisions. Extensive experience collaborating with and advising senior leadership; skilled in leading cross-functional and global initiatives. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at the Director level is $191,000 - $252,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Competitive Intelligence

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking an enthusiastic and highly motivated Associate Scientist to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. Key Responsibilities: Design and execute routine studies to investigate the PK/PD and efficacy of drug candidates. Perform routine to intermediate handling procedures and injections (IV, SC, IP, ICV), monitor animal health, and collect tissues in mice. Perform moderately complex behavioral experiments in animal models. Support the implementation of new animal models. Demonstrate technical proficiency to investigate, identify, develop, optimize, and troubleshoot new methods/ techniques/ instrumentation to address critical project needs. Read and understand scientific literature. Analyze data, author technical reports, and document experiments in an electronic notebook. Participate and present at project team and departmental meetings. Maintain laboratory organization including equipment upkeep and ensure compliance with safety protocols. Collaborate across functional areas. Additional responsibilities as assigned. Required Skills &amp; Experience: BS with 7+ years post-graduate experience or M.S. with 4+ years of experience working in a biotechnology or pharmaceutical company or contract research organization. Experience with molecular biology assays such as qPCR, ELISA, MSD, BCA, Western Blot (or JESS) in tissue matrix. Experience with ISH/IHC assays and analysis. Proficiency with animal handling procedures and injections (IV, SC, IP and ICV). Experience working with animal disease models. Experience in neuroscience. Experience with animal behavioral assays a plus. Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Location: Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This is an in-person position based in Bedford, MA location. Travel: This position will note require travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $110,000 - $131,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Scientist, In vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Sr. Director, Quality Control, will be responsible for providing strategic, scientific, and operational leadership for the QC function at Stoke. This person is accountable for the end-to-end QC strategy, including analytical control and stability strategy, oversight of external QC operations, and regulatory readiness for early clinical development through late-stage development and commercialization. This person is expected to influence Stoke’s quality culture to help reach a sustained state of inspection readiness for all QC related activities. This person will support the development and execution of Stoke’s GMP Quality Management Plan for compliance and risk monitoring for all analytical activities related to Stoke products. This person will be required to contribute to maintaining a fit for purpose and scalable Quality Management System that is always ready for inspection. The ideal candidate is a seasoned QC leader with expertise in analytical method lifecycle management, can demonstrate the ability to successfully work with CMOs and CTLs, and a proven ability to build scalable, phase appropriate processes in a lean environment. This person must have a strong working knowledge of relevant FDA, EU, ICH GMP guidelines and regulations for phase appropriate application for product development. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for product development and quality management is essential to this role. The candidate should be willing to roll up their sleeves and directly contribute to the implementation of processes, technical aspects of development and resolution of issues as required. This role will report into The VP of Quality and will partner closely with other cross-functional groups such as CMC Analytical Development, CMC Manufacturing, Regulatory and other functions within Quality. Key Responsibilities: Aligned with CMC, establish and lead Stoke’s global QC strategy for all stages of product development. Act as a QC subject matter expert and thought partner to senior leadership. Establish and maintain a phase appropriate analytical control strategy to support clinical development, global regulatory filings, and commercialization. Oversee the lifecycle management of analytical methods for oligonucleotide products, including method transfer, qualification, validation, optimization, stability testing, specification justification and maintenance, shelf-life management, reference standard management and remediation. Lead the QC group responsible for routine GMP in-process, release, and stability testing, and establish the long-term quality control testing strategy for clinical and commercial programs. Lead selection, governance, and performance management of CTLs, ensuring excellence with respect to technical support, compliance, and consistent delivery. Partner with CMC, QA, Regulatory Affairs and Program Teams to ensure QC strategies support overall control strategy. Provide leadership and technical input into CMC and analytical sections of regulatory submissions (IND, NDA, IMPD, MAA). As a QC and analytical expert, support interactions with&nbsp;Health Authorities, Stoke Partners and CTLs. Provide GMP compliance interpretation, consultation, training, and ensure data integrity for all QC activities. Mature Stokes’s QC infrastructure, implementing processes and procedures to support the development of therapeutic candidates in a GMP-compliant manner. Drive continuous improvement initiatives across Quality Control. Support Stoke’s GMP inspection preparation program and mock PAI inspections at CTLs and CMOs as necessary Influence Stoke’s Quality culture Lead, mentor, and develop a small team of quality control professionals Required Skills &amp; Experience: BS/BA, MS or PhD in Analytical Sciences or other relevant discipline (i.e., chemistry, biology). 15+ years of progressive experience in QC spanning late-stage development and commercialization and with significant leadership responsibility. Strong working knowledge of GMP, data integrity principles and ICH guidelines. Demonstrated expertise in analytical method validation, lifecycle management, and control strategy development. Experience with regulatory filing preparation and messaging across global jurisdictions Proven ability to set high standards for performance and accountability, ensuring clarity of expectations and outcomes. Effective in conflict resolution/management and negotiation skills. Excellent leadership skills with ability to influence, collaborate and communicate effectively in a cross-functional organization. Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office based in Bedford, MA location. Travel: This position will require approximately 20 % travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $235,000 - $260,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Quality Control

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Sr. Director, Clinical Quality Compliance, will be responsible for overseeing all aspects of GCP and GCLP associated with the development, maintenance and oversight of activities related to Clinical Trials sponsored by Stoke. This role will report into The VP of Quality and will partner closely with other cross-functional groups such as Clinical Development, Clinical Development Operations, DMPK, Toxicology, Regulatory and other functions within Quality to embed GCP quality considerations into clinical development. This person will lead clinical quality strategy across Stoke’s clinical programs. This role ensures global GCP and GCLP compliance (e.g., FDA, EMA, ICH etc.), provides guidance on clinical trial operations, and fosters a culture of quality, compliance, and patient safety. The Senior Director is expected to drive proactive risk identification and mitigation across the clinical development lifecycle, ensuring inspection readiness and effective regulatory engagement. This person will be responsible for establishing Stokes BIMO inspections readiness program and is expected to influence Stoke’s quality culture and help reach a sustained state of inspection readiness. This person will oversee the development and execution of Stoke’s Clinical Quality Management Plan for compliance and risk monitoring for all Stoke clinical studies. This person is required to contribute to establishing a scalable Quality Management System that is always ready for inspection. The ideal candidate will have a strong working knowledge of relevant FDA, EU, ICH GCP/GCLP guidelines and regulations and experience working with CROs and vendors. A demonstrated ability to think outside of the box with a pragmatic risk-based approach for quality management is essential to this role. The candidate should be willing to roll up their sleeves and directly contribute to the implementation of processes and resolution of issues as required. The person should have the capacity to apply QbD clinical development strategies and build and execute an RBQM framework for ensuring data integrity, patient safety and monitoring compliance. Key Responsibilities: Lead clinical quality strategy and execution across clinical programs to ensure compliance with Stoke SOPs and FDA, EMA, and other global regulations. Collaborate with Functional Leaders, Business Process Owners (BPOs), and Clinical Development Operations departments to define, develop, and operationalize SOPs, support implementation, and ensure regulatory compliance Partner with Quality Systems to maintain a fit for purpose eQMS &nbsp;and standardize processes as applicable for GxP compliance management Provide quality oversight and compliance support across non-clinical, clinical, and safety programs, including development of strategic quality oversight plans and participation in study teams Develop and oversee the execution and interpretation of integrated data-driven risk-based quality management plans to monitor the health of compliance. Support CRO and vendor selection, qualification and oversight, including establishment of Quality Agreements Support investigations into scientific misconduct and/or serious breach of GCP Provide GCP compliance interpretation, consultation, training, and other supportive services necessary to maintain and improve the quality of research for timely regulatory submissions. Establish Stoke’s GCP inspection preparation program and facilitate mock PAI inspections as necessary Host GCP BIMO/Health Authority inspections, as necessary, and provide input to responses to regulatory agency findings and questions Contribute to the development of Stoke’s Quality culture Partner with Regulatory Affairs to align quality with regulatory strategy Lead, mentor, and develop a small team of quality professionals Required Skills &amp; Experience:&nbsp; BS/BA, MS or PhD and a minimum of 15 years’ experience, in Biotech, Pharma or CRO Working knowledge of relevant FDA, EU, ICH GCP / GCLP regulations and guidelines Proven capacity to set high standards for performance and accountability, ensuring clarity of expectations and outcomes Demonstrated ability to encourage adaptability and calculated risk-taking, modeling resilience through change Ability to foster transparent, courageous communication and cross-functional collaboration Ability to collaborate effectively in a dynamic, cross-functional matrix environment Conflict resolution/management and negotiation skills Excellent organizational, interpersonal, verbal, and written communication skills Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position, currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 10 % travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $265,000-$290,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Clinical Quality Compliance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The successful candidate will be responsible for overseeing statistical programming activities within Stoke Therapeutics for one or more programs. The Associate Director/Director, Statistical Programming role will work closely with the Biostatistics and Clinical sub teams, by overseeing vendors and working with internal resources to provide statistical programming support data analysis for clinical trials. This individual may participate in departmental and cross functional technology development and process improvement initiatives. This position will report to the Senior Director, Statistical Programming. Key Responsibilities: Work closely with Biostatistics, Data Management and the clinical sub team on the statistical analysis of data to support clinical development, regulatory, and commercialization activities Review CRF to ensure that data collected will meet the objectives and the requirements of statistical analyses Review statistical analysis plans and shells for tables, figures and listings Provide technical and project management leadership for internal statistical programmers or manage CROs to analyze and report complex data in Tables, Listings, and Figures, and for electronic submissions of SDTM, ADaM datasets in CDlSC format Perform ad hoc and exploratory statistical analyses as needed Support regulatory submissions as needed; provide response to regulatory requests independently Support the preparation of publications, including manuscripts, posters and oral presentations Provide oversight of CROs for outsourced statistical programming activities and lead QC activities for key results generated by CROs Provide technical and project management leadership in SAS programs and applications designed to analyze and report complex data in Tables, Listings, and Figures, and for electronic submissions of SDTM, ADaM datasets in CDlSC format. Experience with SAS LSAF is a plus Perform other duties as assigned Required Skills &amp; Experience:&nbsp; MS in biostatistics or a related field with at least 10 years (or BS with 12 years) of relevant experience In-depth knowledge of FDA, EMA and ICH regulations and guidelines Experience with BLAs, MAAs and other regulatory submissions Hands-on expertise in SAS programming, with strong knowledge of CDISC SDTM, ADaM, Define-XML, and FDA submission standards Proven track record of leading clinical studies for statistical programming activities Display highly developed organizational leadership qualities, and proven to work on multi-tasks in fast-paced environment with changing priorities Good communication skills and ability to work with cross-functional study teams Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. &nbsp;This position is a hybrid position currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. &nbsp; The anticipated salary range for this role is $214,000 - $236,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, Statistical Programming

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director, Regulatory CMC is responsible for contributing to the development of Stoke’s products by proposing and implementing global CMC regulatory strategies, leading cross-functional CMC submissions and providing regulatory guidance to cross-functional teams and functional areas for assigned programs from the pre-clinical stage through approval and life-cycle management. This position reports to the Sr. Director of Regulatory CMC. Key Responsibilities: Specific key responsibilities include, but are not limited to: Responsible for developing Regulatory CMC submission strategies and leading cross-functional teams to action on those strategies to meet program and company goals. Lead multidisciplinary teams to prepare high-quality, accurate and compliant CMC sections of regulatory submissions, as well as responses to Regulatory Agency questions Plan, manage, author, review, and obtain stakeholder alignment to facilitate the submission of to support initial INDs/CTAs, marketing applications, supplements and variations Assess and communicate regulatory requirements to ensure all CMC activities comply with applicable regulations and guidelines Proactively and effectively communicate risks and potential mitigations to teams and key stakeholders Provide regulatory support for relevant quality systems such as change control, deviations, and inspection management Assess proposed manufacturing process changes and provide strategic regulatory guidance to enable global implementation Manage and ensure compliance with all reporting requirements, including annual and periodic reports Effectively plan, organize and contribute to formal meetings with global regulatory agencies to ensure alignment on CMC strategies, resolve CMC issues and oversee proactive interactions for initial INDs/CTAs, marketing registration applications, supplements and variations Develop, review, and maintain Regulatory CMC departmental policies and SOPs Maintain up to date knowledge and expertise with FDA/EMA regulations, ICH guidelines, and other international regulatory regulations and guidelines, as needed to support global clinical and commercial programs Additional duties as assigned Required Skills &amp; Experience: Advanced degree in Chemistry or related science (e.g., M.S., Ph.D.) Minimum of 10 years of experience in the biopharmaceutical or pharmaceutical industry Direct experience of the CMC components of IMPD and CTD (IND, NDA, MAA) submissions Basic understanding of oligonucleotide drug development is desirable Experience in managing CMC change control Knowledge and understanding of relevant regulatory and compliance guidelines including those in the US, EU, UK and Canada as well as the ICH guidelines Excellent written and verbal communication skills and attention to detail Demonstrated strong organizational skills including the ability to prioritize tasks and to adhere to timelines. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $ 233,000 – $260,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Regulatory Affairs - CMC

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Scientist will work collaboratively across the Research organization to support preclinical discovery efforts to understand the pharmacokinetics, pharmacodynamics, and efficacy of novel compounds in disease models. This position requires a scientist with deep expertise designing and developing robust assays to investigate preclinical pharmacokinetic, pharmacological, and functional biomarkers in tissue samples. This position will report into the Executive Director of in vivo pharmacology. Key Responsibilities: Develop and optimize preclinical PK,PD, and biomarker assays to support preclinical research efforts and bridge into early development activities. Use a high level of scientific judgment and creative thinking to adapt and/or create standard methods by applying breadth of knowledge and prior work experience. Work collaboratively within and across functions to support programs from early discovery through preclinical proof of concept and candidate selection to advance the company pipeline. Maintain excellent and timely recordkeeping while adhering to aggressive timelines Author study reports that may be used for regulatory filings and scientific manuscripts. Required Skills &amp; Experience: Bachelor’s degree with 16+ years of experience, or Master’s degree with 10+ years of post-graduate experience, or Ph.D. with 6+ (industry experience is required). Degrees in Pharmacology, Neuroscience or relevant disciplines preferred. Deep experience developing and optimizing assays (i.e MSD, ELISA, H-ECL, LCMS, qPCR) in complex matrices, IHC skills a plus. Experience working in drug discovery with an emphasis on neurology indications, experience working with oligonucleotides preferred. Designing and managing experiments at contract research organizations. Experience with PK/PD modeling a plus. Excellent verbal and written communication skills. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is lab-based currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Benefits &amp; Compensation: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $155,00 - $179,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Director, Corporate Quality is responsible for ensuring the effectiveness and continuous improvement of Stoke’s Quality Management System (QMS) throughout the product lifecycle from clinical development to commercialization incompliance with global regulations and guidelines. This person will be responsible for providing strategic and operational leadership for the quality systems function at Stoke, expanding Stoke’s QMS and leading Quality Governance. This person is expected to influence Stoke’s quality culture to help reach a sustained state of inspection readiness for all GxP (GMP, GCP, GLP, GVP) activities. This person will also oversee Stoke’s GxP vendor management and audit programs and manage any regulatory inspections at Stoke. This role will report to the Vice President of Quality and will interface closely with other cross-functional groups such as CMC, Clinical Development, Clinical Development Operations, Program Management, Regulatory, Commercial, IT and other functions within Quality. Key Responsibilities: Aligned with CMC and Clinical Development, establish and lead Stoke’s global compliance strategy for all stages of product development and clinical trial management. Act as a compliance expert and thought partner to senior leadership. Accountable for the end-to-end oversight of Stoke’s QMS that spans the entire product lifecycle and its application to all disciplines of GxP. Oversee the development, implementation, and ongoing effectiveness of Stoke’s QMS in alignment with regulatory expectations across all enterprise functions. Responsible for establishing the processes and communication mechanisms for regulatory intelligence to assess emerging global regulatory expectations for impact on the organization and Stoke’s QMS to ensure ongoing compliance. Establish Quality Governance at an enterprise level by setting meaningful quality metrics and KPIs to monitor performance and sustain improvement. Oversee SOP governance, document control, training management, change management, risk management, data integrity, and GxP computer systems validation. Represent quality on project teams, as needed and provide GxP compliance interpretation, consultation to&nbsp;maintain&nbsp;and improve compliance to ensure product quality, data&nbsp;integrity&nbsp;and patient safety.&nbsp;&nbsp; Oversee&nbsp;Stoke’s Internal and External Audit Program&nbsp;to coordinate required audits,&nbsp;monitor for&nbsp;signals and trends and&nbsp;ensure&nbsp;the implementation of&nbsp;appropriate CAPAs.&nbsp; Help train/prepare Stoke GMP employees for regulatory inspections and host GMP and BIMO regulatory inspections, as necessary, and provide input and review of responses to inspection related regulatory agency questions.&nbsp; Establish and oversee Stoke’s Quality / Regulatory intelligence process to ensure that regulations,&nbsp;standards,&nbsp;and industry best practices are implemented.&nbsp; Partner with CMC, QA, Regulatory Affairs and Program Teams to ensure Quality strategies support overall development and commercial strategy. Champion Stoke’s Quality culture. Lead, mentor, and develop a small team of quality professionals responsible for the administration and management of Stoke’s QMS and compliance management program. Provide expert oversight in guiding remediation and regulatory communications. Required Skills &amp; Experience: BS/BA, MS in life sciences (i.e., Chemistry, Biology, Engineering disciplines) with 15+&nbsp;years&nbsp;progressive&nbsp;experience in Biotech or Pharma setting with experience in&nbsp;either&nbsp;developing,&nbsp;implementing&nbsp;and/or&nbsp;managing&nbsp;GxP&nbsp;Quality Systems.&nbsp; Strong working knowledge of global GxP&nbsp;regulations&nbsp;(FDA, EU, ICH) and guidelines necessary for phase appropriate decision making and representation to regulatory agencies. Proficient&nbsp;in interpreting GxP regulations toward implementing&nbsp;quality&nbsp;systems for&nbsp;GxP&nbsp;applications&nbsp;in a practical manner. Demonstrated ability to work effectively in a matrix environment and drive results through influence as well as direct management. Experience developing strategy and guiding teams to translate strategy into action Proven ability to build scalable, fit for purpose GxP processes in a lean environment Experience dealing with FDA and other major regulatory agencies and leading or&nbsp;participating&nbsp;in hosting regulatory inspections.&nbsp; Possess excellent analytical skills and the ability to communicate complex issues clearly to a broad group of people. Possess a flexible approach to problem solving, be an adept&nbsp;negotiator&nbsp;and apply risk-based&nbsp;pragmatic&nbsp;decisions in a timely manner.&nbsp; Hands-on experience managing small teams, and in providing coaching, development and mentoring to employees.&nbsp; Location(s): Stoke is located in Bedford, MA.&nbsp; This position is a hybrid position with an office based in Bedford, MA location. Travel: This position will require approximately 5-10 % travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $254,000 - $290,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Director, Corporate Quality

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Executive Director/Vice President, Field Leader – Market Access Field National &amp; Regional will be responsible for building and leading Stoke’s market access organization, including Market Access Account Directors (MADs) and Field Reimbursement Managers (FRMs). This leader will develop and execute the field strategy to ensure broad, timely, and sustained access to Zorevunersen across payers and ensure provider sites are compliantly supported on reimbursement processes. The role will focus on establishing trusted relationships with national and regional payers, Commercial and Medicaid, along with healthcare providers to minimize barriers to patient access. As part of the Market Access leadership team, this individual will play a critical role in launch readiness and in shaping the access culture for a first-in-class rare-disease therapy. Key Responsibilities: Leadership &amp; Strategy Build and lead a high-performing market access field access organization, including hiring, developing, and managing MADs and FRMs. Translate corporate access objectives into actionable field plans that align payer, provider, and patient needs. Establish clear goals, metrics, and performance standards for the field team consistent with company objectives and compliance requirements. Payer &amp; Channel Engagement Oversee MADs responsible for engagement with national and regional payers, FFS Medicaid, IDNs to secure favorable coverage and reimbursement. Provide strategic guidance for payer value propositions, opportunities, and pull-through initiatives. Collaborate with Market Access Marketing and Distribution to ensure alignment of payer messages and field materials with overall access strategy. Reimbursement &amp; Patient Access Lead FRMs in educating providers, infusion sites, and office staff on coverage, reimbursement processes, prior authorization, and patient support programs. Partner with the Patient Services and Specialty Distribution to ensure seamless access and reimbursement support for patients and providers. Ensure FRM field activities are compliant, educational, and aligned with corporate policies. Cross-Functional Collaboration Work closely across Commercial, Medical Affairs, and Compliance to ensure consistent and compliant field engagement. Collaborate with Commercial Operations to provide field insights and inform payer strategy, forecasting, and access performance tracking. Partner with Distribution and Patient Services to ensure coordinated communication with external partners impacting patient access. Compliance &amp; Governance Ensure all field team activities adhere to company policies, legal requirements, and industry regulations. Collaborate with Compliance to maintain appropriate field training and certification. Support audit readiness and continuous improvement of compliant field operations.&nbsp; Required Skills &amp; Experience:&nbsp; 12+ years in U.S. market access or reimbursement, including significant field leadership experience. Bachelor’s degree required; advanced degree (MBA, MPH, PharmD) preferred. Proven track record building and leading national payer and reimbursement teams for specialty or rare-disease launches. Deep understanding of payer dynamics, specialty pharmacy networks, buy-and-bill models, and patient access programs. Ability to operate strategically and hands-on in a resource-constrained, rapidly evolving environment. Exceptional leadership and people-development skills. Strong analytical, negotiation, and strategic planning capabilities. Excellent collaboration and communication across internal and external stakeholders. Passion for patient access and rare-disease impact. Location(s): Stoke is located in Bedford, MA and will be moving to a new location in Waltham, MA by the end of 2026.&nbsp; This position is a field-based position.. Travel: This position will require approximately 50 travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at the Executive Director level is $299,000 - $345,000 and at the Vice President level $333,000 - $375,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director/Vice President, Field Leader, Market Access Field National & Regional

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Executive Director/Vice President, Patient Services Lead – Market Access Field will be responsible for developing and leading Stoke’s patient support program for the company’s first commercial launch. This leader will design, implement, and oversee a comprehensive suite of patient and provider support programs that ensure timely, sustained, and compliant access to therapy. Reporting to the Vice President of Market Access, this leader will be responsible for establishing a best-in-class PSS model that integrates external vendors and an internal team of case managers. This individual will oversee the patient access journey — from benefits verification to onboarding, reimbursement support, and ongoing adherence — while ensuring compliance and operational excellence. This is a highly visible role that will shape the patient access model and culture for a rare-disease company bringing its first therapy to market. Key Responsibilities: Strategy &amp; Program Development Design and lead the U.S. Patient Support Services strategy aligned with corporate and market access goals. Build a comprehensive patient access ecosystem, including: Benefit verification and prior authorization support Reimbursement and coverage education Patient onboarding and case management Financial assistance (copay and PAP programs) Specialty pharmacy coordination and data integration Develop KPIs and success metrics to measure and optimize program performance. Team Leadership &amp; Organizational Build-Out Build and lead a team of internal Case Managers and Nurse Educators responsible for direct patient and caregiver support, onboarding, and ongoing case coordination, and compliant clinical education regarding therapy administration and ongoing treatment. Recruit, train, and coach the patient services team to deliver empathetic, compliant, and efficient support. Develop policies, workflows, and quality standards to ensure a consistent, high-touch experience for every patient. Foster a culture of collaboration, accountability, and operational excellence within the PSS team. Vendor Management &amp; Operations Lead the selection, contracting, and management of key patient support vendors (e.g., HUB provider, copay vendor, PAP administrator). Oversee daily operations, ensuring timely patient onboarding, high service quality, and compliance with SLAs. Ensure effective data flows between PSS vendors, specialty pharmacies, and internal systems. Cross-Functional Collaboration Partner closely and compliantly with all appropriate Field Teams, Distribution, and Commercial Operations to ensure a seamless patient access journey. Work with Legal, and Compliance to ensure all patient support activities are compliant with applicable laws, regulations, and company policies. Collaborate with Commercial Operations to generate actionable insights from PSS data. Patient-Centric Design Infuse patient and caregiver feedback into program design to create a differentiated rare-disease experience. Partner with Patient Advocacy to ensure alignment between patient support offerings and community needs. Support the development of educational materials and communication strategies for patients, caregivers, and providers. Compliance &amp; Quality Ensure all programs and communications adhere to FDA, OIG, and industry compliance standards. Maintain up-to-date SOPs governing patient support operations and vendor relationships. Support ongoing audits and risk assessments related to patient services. Required Skills &amp; Experience:&nbsp; 12+ years in patient support, reimbursement, or market access functions within biotechnology or specialty pharmaceuticals; experience in rare disease or ultra-orphan therapies strongly preferred. Bachelor’s degree required; advanced degree (MBA, MPH, or related) preferred. Proven ability to build patient access programs from the ground up and manage vendor-based operations. Deep understanding of hub operations, copay/PAP design, data integration, and compliance frameworks. Entrepreneurial, hands-on leader comfortable operating in a lean, fast-paced environment. Strong operational and project management skills Excellent cross-functional collaboration and communication Analytical thinker with a passion for improving patient access and experience Demonstrated commitment to compliance and patient privacy Location(s): Stoke is located in Bedford, MA and will be moving to a new location in Waltham, MA by the end of 2026.&nbsp; This position is a field-based position. Travel: This position will require approximately 20% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at the Executive Director level is $282,000 - $331,000 and at the Vice President level is $325,000 – 365,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Executive Director/Vice President, Patient Services Lead - Market Access Field

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Director, Development Operations Program Lead drives the clinical development operations program oversight and is accountable for the end-to-end development operational execution of clinical development programs. This role serves as the primary development operational lead at the program level and co-leads the Development Sub Team, translating clinical development strategy into executable global plans and ensuring timely, high-quality delivery of clinical milestones. The Director operates with a strong execution and strategic mindset, identifying and mitigating potential risks, leads program-level development operational decisions, and escalates cross-program, or portfolio level risks to the Senior Director. This role is critical to ensuring consistency, quality, and accountability across study execution in a highly outsourced model. This position reports to the Senior Director, Clinical Development Operations Program Lead. Key Responsibilities: Program-Level Operational Execution Lead the integrated development operations plan for a clinical development program or defined group of studies. Translate clinical development strategy into detailed operational plans, timelines, and resourcing models. Manage program-level risks, dependencies, and critical path activities; proactively identify and escalate issues with proposed mitigations. Provides oversight of CRO, vendor, and site performance metrics within the clinical program. Lead Development Sub-Team and serve as Clinical Development Operations Lead in the Core Program Team. Proactively identify program risks and mitigations, work with cross-functional teams to develop contingency plans and escalate to the Sr. Director, Clinical Development Operations Program Lead. Execute program delivery within established Clinical Development Operations standards, processes, and vendor governance frameworks. Collaborate with external development partners to support alignment on program-level operational plans, timelines, and execution. CRO &amp; Vendor Management Supports the Clinical Study Teams in holding CROs and vendors accountable for program-level performance, quality, timelines, and budget adherence. Coordinate resolution of program-specific CRO or vendor performance issues in collaboration with study teams and functional partners. Escalate systemic or cross-program vendor issues to the Senior Director. Financial &amp; Resource Oversight Support development and monitoring of program-level clinical budgets, ensuring alignment between operational plans, resourcing assumptions, and program priorities, and escalating financial risks as needed. Reporting &amp; Quality Provide program-level operational status reporting, including timelines, risks, and key trade-offs, and prepare materials for program governance forums and milestone reviews. Support program readiness for audits and inspections by maintaining clear documentation of program status, risks, and key operational decisions. Lead program-level operational status reporting, including timelines, risks, and trade-offs. Prepare materials for program governance forums and milestone reviews. Team Leadership &amp; Development Provide program-level leadership and coordination across study-level operational teams to support effective execution of clinical development programs. Foster a collaborative and accountable working environment across internal teams, CRO partners, clinical vendors, and functional stakeholders. Support onboarding of new team members and external partners to the program, ensuring alignment with program objectives and priorities. Required Skills &amp; Experience:&nbsp; Bachelor’s degree required; advanced degree preferred. 10+ years of clinical operations and clinical development operations program management experience, including hands-on trial management and strong understanding of the end-to-end drug development process. Demonstrated experience leading clinical programs in an outsourced CRO model. Strong Strategic mindset with proactive risk management and solution / results orientated. Strong knowledge of GCP, global regulatory requirements, and inspection-readiness expectations. Demonstrated ability to work cross functionally and lead with influence and motivating others. Excellent communication, organizational, and problem-solving skills. Ability to operate independently, manage complexity, and drive execution in a fast-paced environment. Willingness to travel as needed (approximately 15%). Location(s): Stoke is in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position, currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 15% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $236,000 - $260,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Clinical Development Operations Program Lead

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Lab Associate will be responsible for p This position will report into the Senior Director, Facilities &amp; Engineering. This role will be based onsite daily in our Bedford and Waltham, MA locations. Key Responsibilities: Provide support for the overall lab environment, ensuring proper functioning of utilities, lab equipment and life safety systems Perform facility &amp; safety inspections including chemical, biological and universal waste consolidation Handle daily lab waste disposal in accordance with safety regulations and institutional guidelines, ensuring proper segregation, documentation, and coordination with waste management services. Provide support to various facilities administration duties including but not limited to external vendor coordination, landlord services, HVAC, plumbing, etc. Maintain an updated lab inventory, prepare order lists, and maintain sufficient stock of common reagents, supplies, and materials Collaborate closely with cross-functional teams to support lab productivity Manage shipping and receiving of laboratory materials, including biological samples, reagents, and equipment, ensuring compliance with all relevant regulations and documentation requirements. Routine safety shower and eye wash station inspections Performs or supports execution of small repairs as directed Ensures laboratory cleanliness, storing materials in designated areas or properly disposing in accordance with lab guidelines Performs other duties as necessary Required Skills &amp; Experience:&nbsp; Associate’s or Bachelor’s degree preferred with 2–5 years of experience in a lab environment Ability to multitask in a fast-paced environment, prioritize workload effectively, and work well within a team. Self-motivated, well-organized, and reliable individual with a customer-focused mindset. Experience improving scientific workflows. Strong organizational skills and attention to detail. Clear communicator and collaborative teammate with a strong focus on customer satisfaction and maintaining professional relationships. Comfortable working in fast-paced, hands-on lab environments. Strong organizational, planning, and problem-solving skills Ability to lift 50 lbs Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is lab-based currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5 % travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $65,000 - $80,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP). &nbsp; &nbsp; &nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Lab Associate

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The role of the Director, Program Management is to partner with the development Program Leader to drive program planning and execution.&nbsp; The Director will be responsible for providing a combination of drug development, global commercialization, financial, and program management expertise to the Program Team (PT). This role, through partnership with the Program Leader, has significant responsibility for developing, driving, operationalizing, and executing program strategy.&nbsp;&nbsp; The candidate will have prior successful experience managing cross-functional teams in drug development. This individual will also be a strong influencer who can integrate multiple perspectives effectively, align stakeholders across functions and organization levels, problem-solve and achieve the program goals.&nbsp;&nbsp; This position will report into the SVP, Program Leadership and Management. Key Responsibilities: Develop and maintain the integrated program plans, translating development strategy into actionable timelines, milestones, decision points and cross-functional deliverables. Partner closely with the Program Leader to ensure the PT operates efficiently, enabling clear priorities, informed decision-making, and forward planning. Drive program execution by tracking progress against key milestones, identifying emerging risks or dependencies, and facilitating proactive mitigation strategies. Ensure cross-functional alignment across development subteams by coordinating planning, surfacing trade-offs, and clarifying accountabilities. Lead program-level risk identification and mitigation planning, maintaining visibility of key program risks and ensuring they are actively managed by the appropriate teams. Prepare program materials and insights for governance discussions, ensuring leadership has clear, concise information to support strategic decisions. Facilitate effective program team meetings and decision processes, ensuring discussions focus on critical issues, decisions, and execution priorities. Support resource and scenario planning with functional leaders to ensure program plans are achievable and appropriately resourced. Partner with Alliance Management to coordinate collaboration partner interactions, supporting operational alignment, joint governance preparation, and tracking of shared deliverables when applicable. Maintain clear communication of program status, key risks, and upcoming decisions to stakeholders across the organization. Enable effective operation of the PT by driving integration, alignment, and execution across functional subteams throughout the development lifecycle. Required Skills &amp; Experience: Minimum of 10 years total experience in pharmaceutical or biotechnology industry including participation on cross-functional drug development teams. BA/BS degree required; advanced medical, scientific or business degree strongly preferred. Strong understanding of the drug development lifecycle, including clinical development planning, regulatory milestones, and cross-functional dependencies. Demonstrated ability to drive complex programs forward in a matrixed environment, balancing strategic thinking with operational execution. Proven experience integrating cross-functional input into clear, actionable program plans and enabling teams to deliver against shared goals. Strong facilitation and communication skills, with the ability to guide discussions, clarify decisions, and align diverse stakeholders. Ability to anticipate program challenges and proactively develop mitigation strategies. Excellent organizational and analytical skills, with the ability to synthesize complex information into clear insights for leadership. Collaborative leadership style with strong relationship-building skills across functions and external partners. Experience supporting alliance or collaboration programs is a plus. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 20% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $192,000 - $218,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Director, Program Management

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: This role will be responsible for managing the contracting life cycle (assessment, negotiation, finalizing, management and enforcement) for a wide range of types of agreements in support of Stoke Therapeutics’ business operations, while ensuring compliance, alignment with business needs, and operational excellence. The person in this role will need to be able to manage multiple competing priorities and work collaboratively in an environment with multiple stakeholders. The Contracts Specialist will report to the VP, Corporate Legal. Key Responsibilities: Draft, review, and negotiate a wide range of contracts Partner closely with internal stakeholders to ensure contract terms align with company policies, legal and compliance standards, and business objectives Optimize and implement contracting processes and best practices, ensuring efficient workflows, compliance, and alignment across teams Advise internal stakeholders on contractual rights and obligations, as needed Assist with the maintenance of the contract management system, including ensuring proper filing and tracking of contracts, and renewal management and obligation monitoring Develop, update, and maintain contract templates and playbooks in partnership with Legal &amp; Compliance advisors Assist in training and educating employees on contract-related processes and best practices Identify and mitigate potential risks in contract terms and conditions in partnership with Legal &amp; Compliance leadership Required Skills &amp; Experience:&nbsp; Bachelor’s degree in Life Sciences, Business, Law or a related field; candidates with a Paralegal certificate or JD will also be considered. Minimum of 6-8 years of contract review, drafting, negotiation and management experience in a biotech company, Contract Research Organization (CRO), or pharmaceutical environment Technical Knowledge: Proficiency in biopharma research and development matters and familiarity with relevant laws and regulations and intellectual property (IP) matters that arise in the biopharma industry. Proficiency utilizing a contract management system (preferably Agiloft). Other Skills: High attention to detail, strong organizational skills, excellent negotiation and communication skills, proven ability to work with cross-functional teams and build strong internal relationships, and the ability to manage high-volume workloads and competing priorities and meet deadlines in a fast-paced Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position currently based in Bedford and will later be based in Waltham. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role at the Sr. Manager level is $170,000 - $203,000 and at the Associate Director level is $184,000 - $222,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Senior Manager/Associate Director, Contracts Specialist

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Director / Director, Research Operations will serve as a strategic and operational partner to Research leadership, enabling efficient execution of the Research portfolio through strong governance support, portfolio and resource management, and effective vendor oversight. This role will drive rigor, transparency, and operational excellence across Research programs while allowing scientific teams to focus on discovery and innovation. This position will report into the SVP and Head of Research.&nbsp; Key Responsibilities: Research Team &amp; Governance Support Serve as the operational lead for Research team forums, including agenda development, coordination of meeting materials, minutes, and action tracking Plan, coordinate, and execute Scientific &amp; Technical Review Committee (STRC) and other Research governance meetings Partner with Research leadership to ensure effective decision-making, clear documentation, and follow-through on priorities Own Research calendaring, cadence planning, and cross-functional coordination related to governance activities Research Portfolio &amp; Program Management Provide project and portfolio management support across the Research portfolio In partnership with Research Leads, support the development and maintenance of integrated timelines, milestones, and dependencies across Research programs Support resource planning and capacity assessments in collaboration with Research leadership Identify risks, opportunities for acceleration, and scenario-based tradeoffs across the portfolio Prepare portfolio views and summaries to support leadership decision-making Vendor Management &amp; Outsourcing Oversight Support vendor strategy, selection, and oversight for Research-related outsourced activities Coordinate CRO selection processes, including RFP development, evaluation, and onboarding Identify and support sourcing of external capabilities (e.g., NHP sourcing, specialized assays, academic collaborations) Partner with Procurement, Legal, and Finance to support contracting, budgeting, and vendor performance tracking Monitor vendor deliverables, timelines, and issue resolution in collaboration with scientific leads&nbsp; Required Skills &amp; Experience:&nbsp; 6–10+ years of experience in Research Operations, Project Management, or scientific program management within biotech or pharma Strong project management skills, including timeline development, risk management, and resource planning Experience working with external vendors, CROs, and research service providers Ability to translate complex scientific plans into clear operational frameworks and execution plans Exceptional organizational, communication, and stakeholder management skills High degree of judgment, discretion, and comfort operating in a fast-paced, evolving research environment Proficiency with project management and collaboration tools (e.g., MS Project, Smartsheet, PowerPoint, Teams/SharePoint)&nbsp;&nbsp; Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $192,000 - $218,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, Research Operations

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Medical Director, Pharmacovigilance will be responsible for the medical review of adverse event data, the development of company causality assessment frameworks in post-marketing surveillance, and leading signal detection and management activities.&nbsp; This individual will work in close partnership with PV operations to build rigorous, scalable, medical safety practices suited to a clinical development and complex postmarketing environment. This position will report into the Head of Pharmacovigilance. This is a role for a physician who is energized by developing business practice frameworks and review processes that a growing medical safety function requires and leading the medical safety domain within the organization.&nbsp; Rare disease carries meaningful responsibility to patient and families with limited therapeutic options, and the ideal candidate is a safety physician for whom that responsibility is a genuine source of motivation. Key Responsibilities: Medical Case Review Lead medical review of individual case safety reports (ICSR), including serious adverse events (SAE) and adverse events of clinical interest (AECI) across clinical and postmarketing settings; oversee medical review of ICSR performed by vendors/contractors Develop and implement a company causality framework for clinical trial and postmarketing AE data Build and oversee business practices and review workflows that support consistent and inspection-ready medical assessment of ICSR Oversee appropriate MedDRA coding of postmarketing ICSRs and lead the development of product-specific coding guidelines Signal Detection – Safety Governance Lead signal detection and management activities including development/maintenance of product-specific signal detection plans Develop and refine signal detection methodologies appropriate to the postmarketing environment including consideration of data sources, disproportionality analysis Prepare and present signal assessments to the Head of PV and applicable safety governance bodies, with clear benefit-risk contextualization Maintain signal tracking and disposition process ensuring signals are documented, evaluated and closed or escalated in accordance with company procedures and regulatory expectations Serve as the chair of the cross-functional, internal safety review committee (ISRC) responsible for analysis of validated safety signals and for development of recommendations on risk classification and risk mitigation actions to the Stoke Executive Safety Committee (ESC) Regulatory and Aggregate Safety Contribute medical safety content to aggregate safety reports (DSUR, PSUR, PBRER) and NDA/MAA safety summaries with particular responsibility for medical narrative sections and clinical interpretation of safety findings Provide medical input into Company Core Safety Information and safety content of country-specific product labeling Support regulatory agency interactions on medical safety matters Contribute to protocol safety sections, informed consent documents, and investigator brochure safety content Contribute to development and maintenance of reference safety information Organizational Development Collaborate with the Head of Pharmacovigilance and PV Operational leadership on PV departmental capability building, PV System building, developing medical strategies, contributing medical expertise to PV procedures, vendor oversight and safety database governance as it relates to medical review processes Support the development of medical safety staffing requirements and contribute to hiring and onboarding medical safety personnel as the function grows Represent medical safety with clarity and credibility to internal stakeholders across clinical development, medical affairs, regulatory affairs and commercial functions Required Skills &amp; Experience:&nbsp; MD or equivalent medical degree; clinical training in neurology, pediatric neurology, pediatrics is preferred but not required Minimum of 5 years of industry pharmacovigilance experience Experience in post marketing drug safety with exposure to operational and medical complexity of commercial stage AE management and signal detection Experience in signal detection and management including application of clinical judgement to signal interpretation Contribution to aggregate safety reports including NDA or MAA safety content preferred Demonstrated ability to work collaboratively within a structure in which leadership in both medical safety and PV operations contribute complementary expertise in building the PV organization Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid position, currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 10% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $279,000 - $332,000.&nbsp;The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Medical Director, Pharmacovigilance

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Director, Biostatistics is a key contributor to clinical development, providing statistical leadership across all phases of clinical trials (Phases 1-4).&nbsp; They will join a highly collaborative Clinical Development team and partner cross-functionally with Data Management, Clinical Development Operations, and external stakeholders including consultants, CROs, and Key Opinion Leaders. The Associate Director, Biostatistics, will have proven leadership ability in a fast-paced environment, influencing study design and data analysis while ensuring high-quality deliverables. This position will report to the Director of Biostatistics. Key Responsibilities: Lead statistical strategy for clinical trials from protocol development through study reporting, including study design, endpoint selection, sample size determination, and statistical methodology. Author and oversee Statistical Analysis Plans (SAPs) and contribute to Clinical Study Reports (CSRs), ensuring accuracy, quality and regulatory readiness. Analyze and interpret clinical trial data; oversee validation and quality control of TLFs; communicate statistical results and concepts clearly to cross-functional and non-statistical audiences. Partner with cross-functional teams to provide strategic statistical guidance and input across programs from Phase 1 through Phase 3, including regulatory submissions (e.g. New Drug Applications) and post-marketing studies. Contribute to key clinical development and regulatory documents including Clinical Development Plans, protocols, synopses, Investigator Brochures, submissions, and scientific publications. Represent Biostatistics in internal and external forums, including, for example, Investigator Meetings, Advisory Boards, and interactions with Key Opinion Leaders. Coordinate statistical and programming activities with CRO partners to ensure high-quality and timely deliverables. Collaborate closely with Clinical Development, Data Management, Clinical Development Operations, Medical Writing, and other internal departments to achieve alignment across departments. Maintain current knowledge of relevant issues related to statistics and trial design, drug development, Health Authority regulations, and competitive trends to inform strategic decision-making. Required Skills &amp; Experience: Ph.D. in Statistics or Biostatistics with 6+ years of industry experience, or Master’s degree with 8+ years in a clinical trial setting. Strong Knowledge of FDA, EMA, and ICH guidance for drug Excellent working knowledge of CDISC requirements for regulatory submissions, including expertise with preparation and submission of data in STDM, ADaM, and SEND data standards and associated Demonstrated experience across Phase 1 to 3 clinical trial design, execution and data interpretation in both efficacy and safety. Submission experience is a plus. Experience working collaboratively with CROs to ensure high-quality and timely deliverables Excellent interpersonal skills, ability to convey statistical concepts and results to non-statisticians. Proficiency in SAS programming and at least one other programming language such as R for statistical analysis.&nbsp; Experience working in an environment where knowledge of and adherence to SOPs and regulatory guidelines are required. Excellent verbal and written communication Strong attention to detail and process orientation. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $195,000 - $215,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, Biostatistics

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Senior Scientist will work collaboratively across the Research organization to support preclinical discovery efforts to understand the pharmacokinetics, pharmacodynamics, and efficacy of novel compounds in disease models. This position requires a scientist with deep expertise designing and developing robust assays to investigate preclinical pharmacokinetic, pharmacological, and functional biomarkers in tissue samples. This position will report into the Executive Director of in vivo pharmacology. Key Responsibilities: Develop and optimize preclinical PK,PD, and biomarker assays to support preclinical research efforts and bridge into early development activities. Use a high level of scientific judgment and creative thinking to adapt and/or create standard methods by applying breadth of knowledge and prior work experience. Work collaboratively within and across functions to support programs from early discovery through preclinical proof of concept and candidate selection to advance the company pipeline. Maintain excellent and timely recordkeeping while adhering to aggressive timelines Author study reports that may be used for regulatory filings and scientific manuscripts. Required Skills &amp; Experience: Bachelor’s degree with 16+ years of experience, or Master’s degree with 10+ years of post-graduate experience,&nbsp;(industry experience is required). Degrees in Pharmacology, Neuroscience or relevant disciplines preferred. Deep experience developing and optimizing assays (i.e MSD, ELISA, H-ECL, LCMS, qPCR) in complex matrices, IHC skills a plus. Experience working in drug discovery with an emphasis on neurology indications, experience working with oligonucleotides preferred. Designing and managing experiments at contract research organizations. Experience with PK/PD modeling a plus. Excellent verbal and written communication skills. Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is lab-based currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Benefits &amp; Compensation: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $155,00 - $179,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Director, HCP Marketing will report to the Executive Director, Marketing.&nbsp;Along with the Executive Director of Marketing, you will be responsible for all HCP activities from disease education (DE) campaign development and deployment, as well as the creation of the branded campaign for the potential launch of zorevunersen.&nbsp;We are looking for someone with a particular expertise in non-personal marketing, but experience in others areas, such as KOL engagement, market research and congress strategy &amp; execution, are a bonus.&nbsp;As a small company, we operate at a fast pace with many competing priorities so the ability to thrive in this type of requirement is essential. Key Responsibilities: Work with the hiring manager on the development and deployment of the current DE campaign in the second half of 2026. Contribute to the evolution of the strategy and tactics of the DE campaign in anticipation of a potential launch in late 2027, early 2028. Own the media plan and non-personal promotional program and execution, including the development of a KPI plan to assess the success of our efforts and understand where we need to evolve. Provide significant contribution to the development of a HCP branded campaign and tactics. Own the Congress strategy and execution along with Medical Affairs and other key stakeholders Help lead the development and execution of market research that generates actionable insights Provide strategic oversight and leadership in managing relationships with marketing agencies and key external partners, ensuring seamless execution and optimization of marketing initiatives. Foster and strengthen collaborative relationships with key internal stakeholders to ensure success of cross-functional initiatives and long-range plans Lead the MLR process for HCP tactics. Once the Sales team is hired, you will play an active role in working with them in all areas from tactical plan development through to training and rollout at Sales meetings. Required Skills &amp; Experience:&nbsp; A minimum of 5-8 years of experience in HCP Marketing within the biopharma environment Strong track record in developing and executing a non-personal promotional program A proven track record in executing a robust tactical plan that is delivered on time and budget. Experience in a small or medium-sized company is required. Experience in launching a product is preferred. The ability to work autonomously in a fast-paced environment while juggling multiple projects is essential. A strong collaborator who works well with cross-functional colleagues and can foster productive working relationships. This is an individual contributor role and subject to change in the future 30% domestic travel is required. The majority of the travel will be on the East coast Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is remote but please note the travel requirements above. Travel: This position will require approximately 30% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $201,000 - $251,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Associate Director, HCP Marketing

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Medical Director Neurology Clinical Development will provide strategic leadership for planning, execution, and interpretation of clinical studies focused on transformative therapies for life-threatening neurological diseases. This individual will be responsible for leading study design and execution and driving interpretation of data to support development decisions across one or more programs. The Medical Director will serve as a key leader within cross-functional teams and as an important representative of the program with investigators, consultants, and other key stakeholders. This role requires an experienced physician-drug developer who can operate with substantial independence, influence program direction, and ensure scientific and medical rigor from early development through late-stage studies. This role will report to the Vice President, Neurology Clinical Development. Key Responsibilities: Clinical Development &amp; Execution Lead the clinical development strategy for assigned neurology programs, aligning study design and execution with overall program goals, target product profile, and regulatory path Provide medical leadership across the lifecycle of development, from early clinical planning through pivotal studies and submission-enabling activities Lead the design, review, and finalization of protocols, amendments, and other core clinical documents to ensure scientific rigor, feasibility, and strategic alignment Drive study-level data review, interpretation, and presentation to support timely and informed decision-making Identify development risks, anticipate clinical and operational challenges, and proactively propose mitigation strategies Maintain productive working relationships with CROs, investigators, and study site personnel to support enrollment, data quality, and protocol compliance Clinical Oversight &amp; Decision-Making and Scientific Contribution Provide medical oversight for ongoing studies, including medical monitoring strategy, safety review, protocol-related decisions, and clinical issue escalation Partner closely with safety/pharmacovigilance on signal assessment, benefit-risk evaluation, and safety governance Contribute to the development and refinement of Clinical Development Plans and related program materials Oversee the quality and consistency of medical input across study teams, vendors, and collaborators Ensure clinical development activities adherence to GCP, ICH, and applicable regulatory and ethical guidelines Integrate emerging data, literature, and competitive intelligence to inform ongoing program decisions and ensure scientific relevance and rigor Participate in internal discussions and presentations to communicate key scientific insights, data interpretations, and development updates Contribute to the preparation and review of strategic and regulatory documents, including target product profiles, clinical sections of IND/CTA submissions, and briefing packages External Engagement &amp; Communication Engage and collaborate with investigators, consultants, and key opinion leaders to support trial execution and scientific exchange Represent the clinical development organization at investigator meetings, advisory boards, and scientific congresses Contribute to the authorship of abstracts, manuscripts, and scientific presentations communicating clinical findings Leadership &amp; Collaboration Provide clinical and scientific leadership with cross-functional clinical teams, fostering an environment of accountability, curiosity, and collaborative problem solving Contribute meaningfully to governance discussions and development planning Provide strategic clinical input into regulatory strategy, submission planning, and lifecycle management activities Required Skills &amp; Experience: MD or MD/PhD candidates with relevant clinical development experience in neurology, rare/orphan, or genetic diseases Board certified or eligible in neurology, pediatrics, or a relevant specialty strongly preferred Minimum 6 years of relevant experience in biotech or pharmaceutical clinical development, including meaningful responsibility for design, conduct, and medical oversight of clinical trials Strong understanding of FDA/EMA regulations and ICH/GCP guidelines Strong scientific, analytical, and organizational skills Demonstrated ability to independently interpret complex clinical and scientific data and translate insights into development decisions Experience representing programs in cross-functional, partner, and/or regulatory interactions Strong leadership, communication, and influence skills in a matrixed environment High personal integrity, sound judgement, and strong commitment to advancing therapies for patients with serious neurological diseases Location(s): Stoke operates in Bedford, MA. This position is a hybrid position with an office setting based in our Bedford, MA location. Travel: This position will require approximately 20% travel (domestic and international). Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $269,000 - $331,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location.&nbsp; In addition to base salary, Stoke offers an annual bonus and &nbsp;&nbsp;equity participation. Our benefits package includes medical, dental and vision insurance; life, long and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Medical Director, Neurology Clinical Development

Senior Scientist, Ex Vivo Assay Development

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: Stoke is seeking an enthusiastic and highly motivated Research Associate to join the In Vivo Pharmacology Department.&nbsp; This position is an opportunity to learn new techniques and concepts, be creative, and grow with a growing company. This position will report into a Senior Scientist. Key Responsibilities: Support the execution of routine studies to investigate the PK/PD of drug candidates. Perform routine animal procedures including&nbsp;handling, restraint, clinical observations, dosing, sample collection, and necropsy. Perform RNA and protein analysis, including techniques such as qPCR, Western blotting, and related assays Read and understand scientific literature. Maintain accurate, complete, and timely documentation of experimental procedures, observations, and study results. Analyze data, author technical reports, and maintain accurate experimental records and document results in electronic lab notebooks Participate and present at project team and departmental meetings. Collaborate closely with scientists across pharmacology, biology, and translational research teams Required Skills &amp; Experience:&nbsp; S with 2 years post-graduate experience working in a biotechnology or pharmaceutical company or contract research organization or a M.S. Experience with mouse handling and in vivo procedures; intracerebroventricular injection experience a plus. Experience with molecular biology assays such as PCR, MSD, Western Blot (or JESS). Strong organizational skills and attention to detail. Strong verbal and written communication skills. Creative thinking and working well in a team environment. Experience in neuroscience a plus. Experience with ISH/IHC a plus. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This lab-based position is currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 0% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $92,000 - $99,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP). &nbsp;Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Research Associate, in vivo Pharmacology

About Stoke: Stoke Therapeutics (Nasdaq: STOK) is a biotechnology company dedicated to restoring protein expression by harnessing the body’s potential with RNA medicine. Using Stoke’s proprietary approach, the company is developing antisense oligonucleotides (ASOs) to selectively restore naturally occurring protein levels. Stoke’s first medicine in development, zorevunersen, has demonstrated the potential for disease modification in patients with Dravet syndrome and is currently being evaluated in a Phase 3 study. Stoke entered into a strategic collaboration with Biogen in 2025 to develop and commercialize zorevunersen for Dravet syndrome. Under the collaboration, Stoke retains exclusive rights for zorevunersen in the United States, Canada, and Mexico; Biogen receives exclusive rest of world commercialization rights STK-002 is Stoke’s proprietary antisense oligonucleotide (ASO) in clinical development for the treatment of autosomal dominant optic atrophy (ADOA), the most common inherited optic nerve disorder. STK-002 has been granted orphan drug designation by the FDA. A Phase 1 study (OSPREY) of STK-002 in people with ADOA is now underway. The company is also conducting early research in Syngap1, a severe and rare neurodevelopmental disorder, in collaboration with Acadia Pharmaceuticals. Stoke’s initial focus is on diseases of the central nervous system and the eye that are caused by a loss of ~50% of normal protein levels (haploinsufficiency). Proof of concept has been demonstrated in other organs, tissues, and systems, supporting broad potential for the Company’s proprietary approach.Position Purpose: The Associate Director, Biostatistics is a key contributor to clinical development, providing statistical leadership across all phases of clinical trials (Phases 1-4).&nbsp; They will join a highly collaborative Clinical Development team and partner cross-functionally with Data Management, Clinical Development Operations, and external stakeholders including consultants, CROs, and Key Opinion Leaders. The Associate Director, Biostatistics, will have proven leadership ability in a fast-paced environment, influencing study design and data analysis while ensuring high-quality deliverables. This position will report to the Director of Biostatistics. Key Responsibilities: Lead statistical strategy for clinical trials from protocol development through study reporting, including study design, endpoint selection, sample size determination, and statistical methodology. Author and oversee Statistical Analysis Plans (SAPs) and contribute to Clinical Study Reports (CSRs), ensuring accuracy, quality and regulatory readiness. Analyze and interpret clinical trial data; oversee validation and quality control of TLFs; communicate statistical results and concepts clearly to cross-functional and non-statistical audiences. Partner with cross-functional teams to provide strategic statistical guidance and input across programs from Phase 1 through Phase 3, including regulatory submissions (e.g. New Drug Applications) and post-marketing studies. Contribute to key clinical development and regulatory documents including Clinical Development Plans, protocols, synopses, Investigator Brochures, submissions, and scientific publications. Represent Biostatistics in internal and external forums, including, for example, Investigator Meetings, Advisory Boards, and interactions with Key Opinion Leaders. Coordinate statistical and programming activities with CRO partners to ensure high-quality and timely deliverables. Collaborate closely with Clinical Development, Data Management, Clinical Development Operations, Medical Writing, and other internal departments to achieve alignment across departments. Maintain current knowledge of relevant issues related to statistics and trial design, drug development, Health Authority regulations, and competitive trends to inform strategic decision-making. Required Skills &amp; Experience: Ph.D. in Statistics or Biostatistics with 6+ years of industry experience, or Master’s degree with 8+ years in a clinical trial setting. Strong Knowledge of FDA, EMA, and ICH guidance for drug Excellent working knowledge of CDISC requirements for regulatory submissions, including expertise with preparation and submission of data in STDM, ADaM, and SEND data standards and associated Demonstrated experience across Phase 1 to 3 clinical trial design, execution and data interpretation in both efficacy and safety. Submission experience is a plus. Experience working collaboratively with CROs to ensure high-quality and timely deliverables Excellent interpersonal skills, ability to convey statistical concepts and results to non-statisticians. Proficiency in SAS programming and at least one other programming language such as R for statistical analysis. Experience working in an environment where knowledge of and adherence to SOPs and regulatory guidelines are required. Excellent verbal and written communication Strong attention to detail and process orientation. Location(s): Stoke is located in Bedford, MA, and will be moving to a new location in Waltham, MA by the end of 2026. This position is a hybrid currently based in Bedford and will later be based in Waltham. Travel: This position will require approximately 5% travel. Compensation &amp; Benefits: At Stoke Therapeutics, we are committed to offering a comprehensive, competitive and thoughtfully designed total rewards program. The anticipated salary range for this role is $195,000 - $215,000. The final offer will be determined based on a variety of factors, including role scope, experience and qualifications, education, market benchmarks, internal equity, and geographic location. In addition to base salary, Stoke offers an annual bonus and equity participation. Our benefits package includes medical, dental and vision insurance; life, long- and short-term disability insurance; paid parental leave; a 401K plan with company match, unlimited vacation time, tuition assistance and participation in our Employee Stock Purchase Program (ESPP).Culture &amp; Values: At Stoke, we believe that innovation, the ability to successfully advance our ground-breaking science and having fun as a team are enhanced by being together in person, at least periodically. We allow for flexibility in work arrangements that balance individual’s needs and preferences with the needs of our business and our desire to foster a culture of collaboration and innovation. Our values guide our work to deliver meaningful medicines for people who need them. We are committed to being true to ourselves, to our colleagues, and to the people with severe diseases who are counting on us. We embrace diversity within a unique culture that is defined by our values. Our employee-led Diversity, Inclusion, and Belonging (DIB) Committee underscores the importance of DIB to who we are and what we do.&nbsp; Interested candidates: Please visit Stoke’s website to learn more and apply directly to the position listed on our Career Center: https://www.stoketherapeutics.com/careers/ For more information, visit https://www.stoketherapeutics.com/. All applicants will receive consideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, national origin, physical or mental disability or protected veteran status. Stoke participates in E-Verify.

Research and Development Specialist

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