Co-op: Screening & On-target NG

Company Overview: Prime Medicine is a leading biotechnology company dedicated to creating and delivering the next generation of gene editing therapies to patients. The Company is leveraging its proprietary Prime Editing platform, a versatile, precise and efficient gene editing technology, to develop a new class of differentiated, one-time, potentially curative genetic therapies.

Designed to make only the right edit at the right position within a gene while minimizing unwanted DNA modifications, Prime Editors have the potential to repair almost all types of genetic mutations and work in many different tissues, organs and cell types.   Prime Medicine is currently progressing a diversified portfolio of investigational therapeutic programs organized around our core areas of focus: hematology, immunology & oncology, liver and lung.

Across each core area, Prime Medicine is focused initially on a set of high value programs, each targeting a disease with well-understood biology and a clearly defined clinical development and regulatory path, and each expected to provide the foundation for expansion into additional opportunities. For more information, please visit www.primemedicine.com.

Fall 2026 Co-op Program: July – December 2026 Co-op Title: Screening & On-target NGS Manager Name: Sarah Trusiak       Department: Screening & NGS   Assignment Overview Prime Medicine is seeking a highly motivated co-op to join the Screening & NGS team supporting Prime Editing programs.

This role will contribute to high-throughput genome editing experiments in mammalian systems and sequencing-based evaluation of editing outcomes. The candidate will work at the interface of cell biology, genome editing, next-generation sequencing (NGS), and lab automation. In addition to supporting active screening efforts, the co-op will lead a defined project to optimize and automate components of the screening workflow to improve throughput, reproducibility, and data quality.

This position offers hands-on experience with cutting-edge gene editing technologies in a fast-paced, collaborative R&D environment focused on developing transformative genetic medicines. We seek a driven team member passionate about advancing human health.   Primary Responsibilities: Maintain and manipulate mammalian cell cultures using aseptic technique Perform cell transfections and execute gene editing experiments Isolate genomic DNA, prepare NGS libraries, and operate Illumina sequencing platforms Support development of automated liquid handling workflows Analyze and interpret sequencing data to assess editing efficiency and screening performance Maintain accurate experimental documentation using an electronic lab notebook system   Qualifications: Pursuing a B.S. or M.S. in Molecular Biology, Genetics, Bioengineering, or related field Hands-on experience with core molecular biology techniques, including PCR and DNA extraction Experience with aseptic technique Familiarity with genome editing concepts Exposure to NGS workflows or sequencing-based assays Strong attention to detail, troubleshooting ability, and collaborative mindset   Preferred Qualifications: Prior mammalian cell culture experience strongly preferred Hands-on experience with gene editing and/or NGS techniques Experience with laboratory automation and/or data analysis The pay range reflects the full-time base salary range we expect to pay for this role at the time of posting.

Base pay will be determined based on several factors including, but not limited to, relevant experience, skills, and education. This role is eligible for an annual short-term incentive award (e.g., bonus) and an annual long-term incentive award (e.g., equity). Prime Medicine offers a comprehensive Total Rewards package with robust health, financial, and wellness benefits—including medical, dental, vision, life insurance, a 401(k) match, and equity programs—along with generous paid time off, wellness days, and company-wide recharge breaks.

Our commitment to employee well-being reflects our belief that when we care for our people, we strengthen our ability to put patients first and make meaningful impact together. U.S. Pay Range$23—$33 USDPrime Medicine is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, age, sexual orientation, gender identity, disability, veteran status, or any other classification protected by law.

Prime Medicine is a biotechnology company focused on developing innovative RNA-based therapeutics to treat genetic diseases. The company's cutting-edge research and technology aim to revolutionize the field of medicine by providing personalized treatments for patients with rare and common genetic disorders. With a dedicated team of scientists and experts, Prime Medicine is committed to advancing the future of healthcare through its groundbreaking approach to gene therapy.